A Laboratory Guide to the Tight Junction Book

A Laboratory Guide to the Tight Junction

  • Author : Jianghui Hou
  • Publisher : Academic Press
  • Release Date : 2020-04-24
  • Genre: Science
  • Pages : 420
  • ISBN 10 : 9780128226438

A Laboratory Guide to the Tight Junction Excerpt :

A Laboratory Guide to the Tight Junction offers broad coverage of the unique methods required to investigate its characteristics. The methods are described in detail, including its biochemical and biophysical principles, step-by-step process, data analysis, troubleshooting, and optimization. The coverage includes various cell, tissue, and animal models. Chapter 1 provides the foundations of cell biology of tight junction. Chapter 2 covers the Biochemical approaches for paracellular channels and is followed by chapter 3 providing the Biophysical approaches. Chapter 4 describes and discusses Histological approaches for tissue fixation and preparation. Chapter 5 discusses Light microscopy, while chapter 6 presents Electron microscopic approaches. Chapter 7 covers Transgenic manipulation in cell cultures, including DNA and siRNA, Mutagenesis, and viral infection. Chapter 8 covers transgenic manipulation in mice, including: Knockout, Knockin, siRNA knockdown, GFP/LacZ reporter, and overexpression. The final chapter discusses the future developments of new approaches for tight junction research. Researchers and advanced students in bioscience working on topics of cell junction, ion channel and membrane protein will benefit from the described methods. Clinicians and pathologists interested in tissue barrier diseases will also benefit from the biochemical and biophysical characterization of tight junctions in organ systems, and their connection to human diseases. Provides consistent and detailed research methods Covers various cell, tissue and animal models Includes step-by-step guidance from beginner to sophisticated levels

Claudins Book


  • Author : Kursad Turksen
  • Publisher : Humana Press
  • Release Date : 2011-07-02
  • Genre: Science
  • Pages : 447
  • ISBN 10 : 1617791849

Claudins Excerpt :

Since the initial discovery of the claudins, the field of tight junctions and cell polarity has become increasingly energized, mainly due to the fact that the claudins have provided an opportunity to understand the molecular basis of the tight junction function and their tissue specific roles. In Claudins: Methods and Protocols, experts and pioneers in the field provide an extensive collection of techniques involving these tight junction proteins. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include brief introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Claudins: Methods and Protocols serves as an ideal guide for all scientists seeking to further our understanding of this vital area of research.

A Laboratory Guide to the Mammalian Embryo Book

A Laboratory Guide to the Mammalian Embryo

  • Author : David K. Gardner
  • Publisher : Oxford University Press
  • Release Date : 2004-01-08
  • Genre: Science
  • Pages : 394
  • ISBN 10 : 0195142268

A Laboratory Guide to the Mammalian Embryo Excerpt :

Never before has there been such a comprehensive book of protocols. This compendium offers a full range of research techniques-from cell culture, to biochemical, to microscopic and genetic. More focused books, like Cold Spring Harbor's Manipulating the Mouse Embryo, are similar though more narrow in scope. This book will appeal to a broad range of researchers, from basic experimental scientists to clinical and animal scientists.

The Paracellular Channel Book

The Paracellular Channel

  • Author : Jianghui Hou
  • Publisher : Academic Press
  • Release Date : 2018-06-08
  • Genre: Science
  • Pages : 248
  • ISBN 10 : 9780128146361

The Paracellular Channel Excerpt :

The Paracellular Channel: Biology, Physiology and Disease serves as the first volume to offer a cohesive and unifying picture of the critical functions of paracellular channels (tight junctions) in different tissues. This new class of ion channel utilizes a completely different mechanism to create ion passage pathways across the cell junction. This volume outlines common principles that govern the organization and regulation of these diverse cellular structures, describes the methodology of study, and highlights the pathophysiologic consequence of abnormal structure and functions of the paracellular channels in human diseases. Coverage includes biochemical, biophysical, structural, physiologic analyses of the paracellular channel, and new technologies for recording and characterization. Offers integrated coverage of all key aspects of the paracellular channel, an understudied field that may hold key insights into some of the most mysterious aspects of physiology Targets different levels of expertise, spanning from graduate students, interns and clinical fellows, to seasoned researchers that study functions, regulation and dysfunctions of different tissue barriers Provides a cohesive and unifying picture that describes the critical functions of paracellular channels (tight junctions) in different tissues

Human Stem Cell Manual Book

Human Stem Cell Manual

  • Author : Suzanne Peterson
  • Publisher : Academic Press
  • Release Date : 2012-10-22
  • Genre: Science
  • Pages : 656
  • ISBN 10 : 9780123854742

Human Stem Cell Manual Excerpt :

This manual is a comprehensive compilation of "methods that work" for deriving, characterizing, and differentiating hPSCs, written by the researchers who developed and tested the methods and use them every day in their laboratories. The manual is much more than a collection of recipes; it is intended to spark the interest of scientists in areas of stem cell biology that they may not have considered to be important to their work. The second edition of the Human Stem Cell Manual is an extraordinary laboratory guide for both experienced stem cell researchers and those just beginning to use stem cells in their work. Offers a comprehensive guide for medical and biology researchers who want to use stem cells for basic research, disease modeling, drug development, and cell therapy applications. Provides a cohesive global view of the current state of stem cell research, with chapters written by pioneering stem cell researchers in Asia, Europe, and North America. Includes new chapters devoted to recently developed methods, such as iPSC technology, written by the scientists who made these breakthroughs.

The Impact of Food Bioactives on Health Book

The Impact of Food Bioactives on Health

  • Author : Kitty Verhoeckx
  • Publisher : Springer
  • Release Date : 2015-04-29
  • Genre: Technology & Engineering
  • Pages : 338
  • ISBN 10 : 9783319161044

The Impact of Food Bioactives on Health Excerpt :

“Infogest” (Improving Health Properties of Food by Sharing our Knowledge on the Digestive Process) is an EU COST action/network in the domain of Food and Agriculture that will last for 4 years from April 4, 2011. Infogest aims at building an open international network of institutes undertaking multidisciplinary basic research on food digestion gathering scientists from different origins (food scientists, gut physiologists, nutritionists...). The network gathers 70 partners from academia, corresponding to a total of 29 countries. The three main scientific goals are: Identify the beneficial food components released in the gut during digestion; Support the effect of beneficial food components on human health; Promote harmonization of currently used digestion models Infogest meetings highlighted the need for a publication that would provide researchers with an insight into the advantages and disadvantages associated with the use of respective in vitro and ex vivo assays to evaluate the effects of foods and food bioactives on health. Such assays are particularly important in situations where a large number of foods/bioactives need to be screened rapidly and in a cost effective manner in order to ultimately identify lead foods/bioactives that can be the subject of in vivo assays. The book is an asset to researchers wishing to study the health benefits of their foods and food bioactives of interest and highlights which in vitro/ex vivo assays are of greatest relevance to their goals, what sort of outputs/data can be generated and, as noted above, highlight the strengths and weaknesses of the various assays. It is also an important resource for undergraduate students in the ‘food and health’ arena.

Human Neuroanatomy Book

Human Neuroanatomy

  • Author : J. Edward Bruni
  • Publisher : Oxford University Press, USA
  • Release Date : 2009
  • Genre: Medical
  • Pages : 342
  • ISBN 10 : 9780195371420

Human Neuroanatomy Excerpt :

The Human Brain in Dissection will significantly update the previous edition published in 1988. The last 20 years have sen a significant shift in the way that neuroanatomy is taught in both undergraduate and graduate neuroscience courses, as well as doctorate courses: not only has the time allocated for these courses been reduced, but the methodologies for teaching have become more focused and specific due to these time constraints. The Human Brain in Dissection, Third Edition will provide detailed features of the human brain with the above limitations in mind. 50 new plates will be added to the existing 123 in order to permit the student to see all salient structures and to visualize microscopic structures of the brain stem and spinal cord. Each chapter will cover a specific are of the human brain in such a way that each chapter can be taught in one two-hour neuroanatomy course. New to this edition is the inclusion of a section in each chapter on clinically relevant examples. Each chapter will also include a specific laboratory exercise. And finally, the author has included a question and answer section that is relevant to the USMLE, as as recommended readings, neither of which were included in the previous editions. This new edition of The Human Brain in Dissection will allow the student to: understand basic principles of cellular neuroscience; learn gross and microscopic anatomy of the central nervous system (Brain, brainstem, and spinal cord); relate the anatomy of central neural pathways to specific functional systems; be able to localize and name a CNS legion when presented with neurological symptoms, and appreciate higher cortical functions and how they relate to the practice of neurology. neuroscience

NIH Guide for Grants and Contracts Book

NIH Guide for Grants and Contracts

  • Author : National Institutes of Health (U.S.)
  • Publisher : Unknown
  • Release Date : 2022-07-01
  • Genre: Research grants
  • Pages : null
  • ISBN 10 : STANFORD:36105219414625

NIH Guide for Grants and Contracts Excerpt :

General Zoology Laboratory Guide Book

General Zoology Laboratory Guide

  • Author : Charles F. Lytle
  • Publisher : McGraw-Hill Science, Engineering & Mathematics
  • Release Date : 2004-02-28
  • Genre: Science
  • Pages : 372
  • ISBN 10 : 007234900X

General Zoology Laboratory Guide Excerpt :

General Zoology Laboratory Manual is ideal for the laboratory that emphasizes the dissection and microscopic study of live and preserved specimens. Recognized for its accuracy and readability, this manual is comprehensive in its representation of the major groups of animal phyla.This new edition is suitable for a wide range of course needs and structures.

Skin Barrier Function Book

Skin Barrier Function

  • Author : T. Agner
  • Publisher : Karger Medical and Scientific Publishers
  • Release Date : 2016-02-04
  • Genre: Medical
  • Pages : 164
  • ISBN 10 : 9783318055863

Skin Barrier Function Excerpt :

Although a very fragile structure, the skin barrier is probably one of the most important organs of the body. Inward/out it is responsible for body integrity and outward/in for keeping microbes, chemicals, and allergens from penetrating the skin. Since the role of barrier integrity in atopic dermatitis and the relationship to filaggrin mutations was discovered a decade ago, research focus has been on the skin barrier, and numerous new publications have become available. This book is an interdisciplinary update offering a wide range of information on the subject. It covers new basic research on skin markers, including results on filaggrin and on methods for the assessment of the barrier function. Biological variation and aspects of skin barrier function restoration are discussed as well. Further sections are dedicated to clinical implications of skin barrier integrity, factors influencing the penetration of the skin, influence of wet work, and guidance for prevention and saving the barrier. Distinguished researchers have contributed to this book, providing a comprehensive and thorough overview of the skin barrier function. Researchers in the field, dermatologists, occupational physicians, and related industry will find this publication an essential source of information.

A Comprehensive Guide to Toxicology in Nonclinical Drug Development Book

A Comprehensive Guide to Toxicology in Nonclinical Drug Development

  • Author : Ali S. Faqi
  • Publisher : Academic Press
  • Release Date : 2016-11-03
  • Genre: Medical
  • Pages : 986
  • ISBN 10 : 9780128036211

A Comprehensive Guide to Toxicology in Nonclinical Drug Development Excerpt :

A Comprehensive Guide to Toxicology in Nonclinical Drug Development, Second Edition, is a valuable reference designed to provide a complete understanding of all aspects of nonclinical toxicology in the development of small molecules and biologics. This updated edition has been reorganized and expanded to include important topics such as stem cells in nonclinical toxicology, inhalation and dermal toxicology, pitfalls in drug development, biomarkers in toxicology, and more. Thoroughly updated to reflect the latest scientific advances and with increased coverage of international regulatory guidelines, this second edition is an essential and practical resource for all toxicologists involved in nonclinical testing in industry, academic, and regulatory settings. Provides unique content that is not always covered together in one comprehensive resource, including chapters on stem cells, abuse liability, biomarkers, inhalation toxicology, biostatistics, and more Updated with the latest international guidelines for nonclinical toxicology in both small and large molecules Incorporates practical examples in order to illustrate day-to-day activities and the expectations associated with working in nonclinical toxicology

Molecular Biology of the Cell Book
Score: 4.5
From 5 Ratings

Molecular Biology of the Cell

  • Author : Bruce Alberts
  • Publisher : Unknown
  • Release Date : 2004
  • Genre: Uncategoriezed
  • Pages : null
  • ISBN 10 : 0815332181

Molecular Biology of the Cell Excerpt :

The Laboratory Guide Book

The Laboratory Guide

  • Author : Sir Arthur Herbert CHURCH
  • Publisher : Unknown
  • Release Date : 1894
  • Genre: Chemistry
  • Pages : 292
  • ISBN 10 : WISC:89043723543

The Laboratory Guide Excerpt :