Basic Molecular Protocols in Neuroscience Book

Basic Molecular Protocols in Neuroscience


  • Author : John T. Corthell
  • Publisher : Academic Press
  • Release Date : 2014-04-16
  • Genre: Medical
  • Pages : 124
  • ISBN 10 : 012801461X

DOWNLOAD BOOK
Basic Molecular Protocols in Neuroscience Excerpt :

Basic Neuroscience Protocols: Tips, Tricks, and Pitfalls contains explanatory sections that describe the techniques and what each technique really tells the researcher on a scientific level. These explanations describe relevant controls, troubleshooting, and reaction components for some of the most widely used neuroscience protocols that remain difficult for many neuroscientists to implement successfully. Having this additional information will help researchers ensure that their experiments work the first time, and will also minimize the time spent working on a technique only to discover that the problem was them, and not their materials. Describes techniques in very specific detail with Step-by-step instructions, giving researchers in depth understanding Offers many details not present in other protocol books Describes relevant controls for each technique and what those controls mean Chapters include references (key articles, books, protocols) for additional study Describes both the techniques and the habits necessary to get quality results, such as aseptic technique, aliquoting, and general laboratory rules

Basic Molecular Protocols in Neuroscience  Tips  Tricks  and Pitfalls Book

Basic Molecular Protocols in Neuroscience Tips Tricks and Pitfalls


  • Author : John T. Corthell
  • Publisher : Academic Press
  • Release Date : 2014-04-16
  • Genre: Science
  • Pages : 124
  • ISBN 10 : 9780128015278

DOWNLOAD BOOK
Basic Molecular Protocols in Neuroscience Tips Tricks and Pitfalls Excerpt :

Basic Neuroscience Protocols: Tips, Tricks, and Pitfalls contains explanatory sections that describe the techniques and what each technique really tells the researcher on a scientific level. These explanations describe relevant controls, troubleshooting, and reaction components for some of the most widely used neuroscience protocols that remain difficult for many neuroscientists to implement successfully. Having this additional information will help researchers ensure that their experiments work the first time, and will also minimize the time spent working on a technique only to discover that the problem was them, and not their materials. Describes techniques in very specific detail with step-by-step instructions, giving researchers in-depth understanding Offers many details not present in other protocol books Describes relevant controls for each technique and what those controls mean Chapters include references (key articles, books, protocols) for additional study Describes both the techniques and the habits necessary to get quality results, such as aseptic technique, aliquoting, and general laboratory rules

Family Iridoviridae Molecular and Ecological Studies of a Family Infecting Invertebrates and Ectothermic Vertebrates Book

Family Iridoviridae Molecular and Ecological Studies of a Family Infecting Invertebrates and Ectothermic Vertebrates


  • Author : V. Gregory Chinchar
  • Publisher : MDPI
  • Release Date : 2019-10-01
  • Genre: Science
  • Pages : 234
  • ISBN 10 : 9783039215164

DOWNLOAD BOOK
Family Iridoviridae Molecular and Ecological Studies of a Family Infecting Invertebrates and Ectothermic Vertebrates Excerpt :

Ranaviruses and other viruses within the family Iridoviridae, infect a wide range of ecologically and commercially important ectothermic vertebrates, i.e., bony fish, amphibians, and reptiles, and invertebrates, including agricultural and medical pests and cultured shrimp and crayfish, and are responsible for considerable morbidity and mortality. Understanding the impact of these various agents on diverse host species requires the combined efforts of ecologists, veterinarians, pathologists, comparative immunologists and molecular virologists. Unfortunately, investigators involved in these studies often work in discipline-specific silos that preclude interaction with others whose insights and approaches are required to comprehensively address problems related to ranavirus/iridovirus disease. Our intent here is to breakdown these silos and provide a forum where diverse researchers with a common interest in ranavirus/iridovirus biology can profitably interact. As a colleague once quipped, “Three people make a genius.” We are hoping to do something along those lines by presenting a collection of research articles dealing with issues of anti-viral immunity, identification of a potentially novel viral genus exemplified by erythrocytic necrosis virus, viral inhibition of innate immunity, identification of novel hosts for lymphocystivirus and invertebrate iridoviruses, and modelling studies of ranavirus transmission. Collectively these and others will exemplify the breadth of ongoing studies focused on this virus family.

Guide to Research Techniques in Neuroscience Book

Guide to Research Techniques in Neuroscience


  • Author : Matt Carter
  • Publisher : Academic Press
  • Release Date : 2022-04-08
  • Genre: Medical
  • Pages : 414
  • ISBN 10 : 9780323915618

DOWNLOAD BOOK
Guide to Research Techniques in Neuroscience Excerpt :

Modern neuroscience research is inherently multidisciplinary, with a wide variety of cutting edge new techniques to explore multiple levels of investigation. This Third Edition of Guide to Research Techniques in Neuroscience provides a comprehensive overview of classical and cutting edge methods including their utility, limitations, and how data are presented in the literature. This book can be used as an introduction to neuroscience techniques for anyone new to the field or as a reference for any neuroscientist while reading papers or attending talks. • Nearly 200 updated full-color illustrations to clearly convey the theory and practice of neuroscience methods • Expands on techniques from previous editions and covers many new techniques including in vivo calcium imaging, fiber photometry, RNA-Seq, brain spheroids, CRISPR-Cas9 genome editing, and more • Clear, straightforward explanations of each technique for anyone new to the field • A broad scope of methods, from noninvasive brain imaging in human subjects, to electrophysiology in animal models, to recombinant DNA technology in test tubes, to transfection of neurons in cell culture • Detailed recommendations on where to find protocols and other resources for specific techniques • “Walk-through boxes that guide readers through experiments step-by-step

Emerging Fungal Plant Pathogens Book

Emerging Fungal Plant Pathogens


  • Author : Samantha Chandranath Karunarathna
  • Publisher : Frontiers Media SA
  • Release Date : 2021-11-05
  • Genre: Science
  • Pages : 159
  • ISBN 10 : 9782889715947

DOWNLOAD BOOK
Emerging Fungal Plant Pathogens Excerpt :

Molecular Neuroscience Book

Molecular Neuroscience


  • Author : Rusty Lansford
  • Publisher : Unknown
  • Release Date : 2014-09-30
  • Genre: Science
  • Pages : 632
  • ISBN 10 : 1621820130

DOWNLOAD BOOK
Molecular Neuroscience Excerpt :

A wide variety of powerful molecular techniques have been applied to biology in recent decades, ranging from recombinant DNA technologies to state-of-the-art imaging methods. But the plethora of techniques available combined with the complexities of neurobiological systems can make it difficult for neuroscientists to select and carry out an experimental procedure to effectively address the question at hand. This laboratory manual serves as a comprehensive practical guide to molecular and cellular methods for neuroscientists. It consists of five major sections: Working with Cells, Working with DNA, Working with RNA, Gene Transfer, and Imaging. Each includes step-by-step protocols and discussions of basic and cutting-edge procedures for working in that area. Fundamental techniques include maintaining a sterile working environment, purifying and culturing neural cells, isolating and manipulating DNA and RNA, and understanding and using a microscope. Advanced topics include single-neuron isolation and analysis, in vivo gene delivery and imaging, optogenetics, RNA interference, transgenic technologies, high-throughput analysis of gene expression (e.g., RNA-Seq), and constructing and imaging fluorescent proteins. The manual includes protocols developed in the Advanced Techniques in Molecular Neuroscience course offered annually at Cold Spring Harbor Laboratory, as well as protocols drawn from its best-selling lab manuals. It is an essential resource for all neuroscientists, from graduate students upward, who seek to use molecular techniques to probe the complexities of the nervous system.

Current Protocols in Neuroscience Book

Current Protocols in Neuroscience


  • Author : Jacqueline N. Crawley
  • Publisher : Current Protocols
  • Release Date : 1997-06-01
  • Genre: Medical
  • Pages : null
  • ISBN 10 : 0471163619

DOWNLOAD BOOK
Current Protocols in Neuroscience Excerpt :

Current Protocols in Neuroscience (CPN) draws from techniques in molecular neurobiology, neurophysiology, neuroanatomy, neuropharmacology, and behavioral neuroscience to meet the specific needs of researchers in the full range of disciplines that is involved in studying the brain, nervous system, and corresponding behaviors. The editorial board of CPN have assembled an outstanding range of methods to enable users to explore their fields in greater depth and branch into related areas. The one-volume, looseleaf manual features carefully edited techniques with authors' troubleshooting tips and helpful comments that come from extensive experience in using these procedures. Quarterly updates, filed into the looseleaf, keep you and your laboratory current with the latest developments in this rapidly changing field. The initial purchase includes one year of updates and then subscribers may renew their annual subscriptions. Current Protocols publishes a family of laboratory manuals for bioscientists, including Molecular Biology, Immunology, Human Genetics, Protein Science, Cytometry, Cell Biology, Pharmacology, and Toxicology.

Poly ADP Ribose  Polymerase Book

Poly ADP Ribose Polymerase


  • Author : Alexei V. Tulin
  • Publisher : Springer Nature
  • Release Date : 2023
  • Genre: NAD-ADP-ribosyltransferase
  • Pages : 441
  • ISBN 10 : 9781071628911

DOWNLOAD BOOK
Poly ADP Ribose Polymerase Excerpt :

This detailed volume explores poly(ADP-ribose) polymerases (PARPs) in the biology of eukaryotes and their relevance to human health. Beginning with a section on the detection and quantification of poly(ADP-ribose) polymer (pADPr), the book continues by delving into the identification of protein targets, functional analysis, the poly(ADP-ribosyl)ating pathway in chromatin and genes expression, as well as the use of animal models and PARP1 inhibitor design and testing, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Poly(ADP-Ribose) Polymerase: Methods and Protocols, Third Edition presents essential new and classical methods for studying the pADPr-pathway.

Immunotherapy for Gastrointestinal Malignancies Book

Immunotherapy for Gastrointestinal Malignancies


  • Author : Ramakrishna Vadde
  • Publisher : Springer Nature
  • Release Date : 2021-01-31
  • Genre: Medical
  • Pages : 251
  • ISBN 10 : 9789811564871

DOWNLOAD BOOK
Immunotherapy for Gastrointestinal Malignancies Excerpt :

This book reviews current immunotherapeutic strategies for gastrointestinal (GI) malignancies, including immune composition, immune checkpoint inhibitors, cell therapy, and peptide vaccines used to protect against esophageal, gastric, hepato-biliary, pancreatic and colorectal cancers. It also discusses the current challenges of using immunotherapy for the treatment of gastrointestinal malignancies. The book reviews highly sensitive and specific immunomarkers for the detection of GI malignancies, and examines therapeutic vaccines and the major cytokines involved in GI immunotherapy, as well as their basic biology and clinical applications. In closing, the book explores various aspects of computational biology for the detection and treatment of GI malignancies.

Contemporary Methods in Bioinformatics and Biomedicine and Their Applications Book

Contemporary Methods in Bioinformatics and Biomedicine and Their Applications


  • Author : Sotir S. Sotirov
  • Publisher : Springer Nature
  • Release Date : 2022
  • Genre: Bioinformatics
  • Pages : 457
  • ISBN 10 : 9783030966386

DOWNLOAD BOOK
Contemporary Methods in Bioinformatics and Biomedicine and Their Applications Excerpt :

This book gathers selected papers from the First International Symposium on Bioinformatics and Biomedicine. Issues related to medicine and health care constitute one of the grand challenges faces by the mankind, and this naturally implies a growing interest in these problems among both researchers and scholars, politicians and policymakers, as well as economist. The present values which gather selected papers from the First International Symposium on Bioinformatics and Biomedicine (BioInfoMed'2020) is a recent response to this interests. In the subsequent sections and chapters, it covers a multitude of various topics related to bioinformatics, biomedicine, bioenginering, as well as a broadly perceives healthcare. Issues related to decision making in bioinformatics, biomedicine and health care, mathematical modelling in biomedicine and health care, artificial intelligence in biomedicine and health care, uncertainty and imprecision, notably intuitionistic fuzzy sets with applications in bioinformatics and biomedicine, biomedical approaches and applications, biomedical imaging and image processing, and excitable structures and motor activity are covered.

Protocols used in Molecular Biology Book

Protocols used in Molecular Biology


  • Author : Sandeep Singh
  • Publisher : Bentham Science Publishers
  • Release Date : 2020-01-23
  • Genre: Science
  • Pages : 186
  • ISBN 10 : 9789811439292

DOWNLOAD BOOK
Protocols used in Molecular Biology Excerpt :

Protocols used in Molecular Biology is a compilation of several examples of molecular biology protocols. Each example is presented with a concise introduction, materials and chemicals required, a step-by-step procedure and troubleshooting tips. Information about the application of the protocol is also provided. The techniques included in this book are essential to research in the fields of proteomics, genomics, cell culture, epigenetic modification and structural biology. The protocols can also be used by clinical researchers (neuroscientists and oncologists, for example) for medical applications (diagnostics, therapeutics and multidisciplinary projects).

Basic Science Methods for Clinical Researchers Book

Basic Science Methods for Clinical Researchers


  • Author : Morteza Jalali
  • Publisher : Academic Press
  • Release Date : 2017-03-31
  • Genre: Science
  • Pages : 382
  • ISBN 10 : 9780128030783

DOWNLOAD BOOK
Basic Science Methods for Clinical Researchers Excerpt :

Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow’s clinician scientists and future leaders in discovery science. Serves as a helpful guide for clinical researchers who lack a conventional science background Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)

Cellular and Molecular Methods in Neuroscience Research Book

Cellular and Molecular Methods in Neuroscience Research


  • Author : Adalberto Merighi
  • Publisher : Springer Science & Business Media
  • Release Date : 2007-12-14
  • Genre: Medical
  • Pages : 303
  • ISBN 10 : 9780387224602

DOWNLOAD BOOK
Cellular and Molecular Methods in Neuroscience Research Excerpt :

There are numerous books on cellular and molecular protocols for general use in cell biology but very few are exclusively devoted to neurobiology. This book fills this gap and explains in a clear and consistent manner, some of the more commonly used protocols in neuroscience research. Each chapter is written by either the person who invented the procedure or an expert in the field. The format is uniform: "Overview," "Background," "Protocols," and "results and discussion." Each protocol begins with the principle of the technique, studies in cell culture, materials and reagents, and, lastly, step-by-step outline of the procedure itself. This highly practical book is also well illustrated (with 17 four color plates) to make the concepts and procedures easy to understand and perform.

Basic Stereology for Biologists and Neuroscientists Book

Basic Stereology for Biologists and Neuroscientists


  • Author : Mark J. West
  • Publisher : Unknown
  • Release Date : 2012
  • Genre: Science
  • Pages : 203
  • ISBN 10 : 1936113600

DOWNLOAD BOOK
Basic Stereology for Biologists and Neuroscientists Excerpt :

Stereological approaches allow researchers to estimate the number, size and shapes of cellular structures, neurons, and other features of biological tissue viewed under the microscope, using sampling techniques to provide a quantitative three-dimensional picture. This book explains how to design and evaluate stereological studies, covering the basic theoretical principles as well as practical issues such as how to get started, pilot studies, and tissue preparation.