Bioconjugate Techniques Book
Score: 5
From 1 Ratings

Bioconjugate Techniques


  • Author : Greg T. Hermanson
  • Publisher : Academic Press
  • Release Date : 2013-07-25
  • Genre: Science
  • Pages : 1200
  • ISBN 10 : 9780123822406

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Bioconjugate Techniques Book Description :

Bioconjugate Techniques, 3rd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions, with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. Offers a one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Provides step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates Features full color illustrations Includes a more extensive introduction into the vast field of bioconjugation and one of the most thorough overviews of immobilization chemistry ever presented

Bioconjugate Techniques Book
Score: 5
From 4 Ratings

Bioconjugate Techniques


  • Author : Greg T. Hermanson
  • Publisher : Academic Press
  • Release Date : 2010-07-26
  • Genre: Science
  • Pages : 1323
  • ISBN 10 : 0080568726

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Bioconjugate Techniques Book Description :

Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticles Silane coupling agents Dendrimers and dendrons Chemoselective ligation Quantum dots Lanthanide chelates Cyanine dyes Discrete PEG compounds Buckyballs,fullerenes, and carbon nanotubes Mass tags and isotope tags Bioconjugation in the study of protein interactions

Drug like Properties  Concepts  Structure Design and Methods Book

Drug like Properties Concepts Structure Design and Methods


  • Author : Li Di
  • Publisher : Elsevier
  • Release Date : 2010-07-26
  • Genre: Science
  • Pages : 552
  • ISBN 10 : 9780080557618

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Drug like Properties Concepts Structure Design and Methods Book Description :

Of the thousands of novel compounds that a drug discovery project team invents and that bind to the therapeutic target, typically only a fraction of these have sufficient ADME/Tox properties to become a drug product. Understanding ADME/Tox is critical for all drug researchers, owing to its increasing importance in advancing high quality candidates to clinical studies and the processes of drug discovery. If the properties are weak, the candidate will have a high risk of failure or be less desirable as a drug product. This book is a tool and resource for scientists engaged in, or preparing for, the selection and optimization process. The authors describe how properties affect in vivo pharmacological activity and impact in vitro assays. Individual drug-like properties are discussed from a practical point of view, such as solubility, permeability and metabolic stability, with regard to fundamental understanding, applications of property data in drug discovery and examples of structural modifications that have achieved improved property performance. The authors also review various methods for the screening (high throughput), diagnosis (medium throughput) and in-depth (low throughput) analysis of drug properties. * Serves as an essential working handbook aimed at scientists and students in medicinal chemistry * Provides practical, step-by-step guidance on property fundamentals, effects, structure-property relationships, and structure modification strategies * Discusses improvements in pharmacokinetics from a practical chemist's standpoint

Bioconjugation Protocols Book

Bioconjugation Protocols


  • Author : Sonny S. Mark
  • Publisher : Humana Press
  • Release Date : 2016-08-23
  • Genre: Science
  • Pages : 605
  • ISBN 10 : 1493960962

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Bioconjugation Protocols Book Description :

Contemporary approaches to the synthesis of chemically modified biomacromolecules (proteins, nucleic acids, lipids, and carbohydrates) not only require efficient means to control conjugation and the specific site of attachment of the conjugated moiety but also the effective use of recent developments in the fields of pharmaceutical chemistry, biomolecular/polymer engineering, and nanobiotechnology. In this second edition of Bioconjugation Protocols: Strategies and Methods, expert researchers update the classic methods and introduce valuable new approaches that go beyond basic conjugation techniques to include elements from advanced organic synthesis, molecular biology, surface biotechnology, materials science, and nanobioscience/engineering. These readily reproducible methods cover the preparation of biomolecular conjugates using a variety of labeling techniques and semisynthetic approaches. Additional chapters address the biofunctionalization of surface structures, including organic/inorganic thin films, as well as various types of nanostructures (magnetic nanoparticles, quantum dots, carbon nanotubes, and silicon nanowire devices). All the protocols follow the successful Methods in Molecular BiologyTM series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Bioconjugation Protocols: Strategies and Methods, Second Edition offers both novice and experienced researchers access to the broad array of techniques needed to carry out the semisynthesis of functional biomolecular reagents and/or the biofunctionalization of surfaces and structures of unique interest for a wide variety of applications, ranging from novel biomedical diagnostics to powerful new therapeutics to advanced biomaterials.

Immobilized Affinity Ligand Techniques Book

Immobilized Affinity Ligand Techniques


  • Author : Greg T. Hermanson
  • Publisher : Academic Press
  • Release Date : 1992-09-10
  • Genre: Science
  • Pages : 454
  • ISBN 10 : STANFORD:36105020876368

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Immobilized Affinity Ligand Techniques Book Description :

This book is a practical guide to the preparation and use of immobilized affinity ligands for purification, catalysis, and analysis. Special emphasis is given to immunochemical techniques including antibody isolation, preparation of antibody fragments using immobilized enzymes, and immunoaffinity chromatography. The book provides easy-to-follow, well-tested protocols to allow the uninitiated to use these techniques to the maximum advantage with minimum hassle. In addition, it shows researchers how to save money by making their own optimized affinity supports. Matrix activation: Ligand immobilization, Binding and elution of target molecules, Enzyme catalysis on solid supports, Analytical affinity chromatography, Isolation/purification of antibodies, Preparation of antibody fragments, Immunoaffinity chromatography, Immobilization of nucleic acids, Use of immobilized ligands for removal of trace contaminants Practical advice on choosing: Matrices, Spacers, Methods of activation and coupling Background information and insights on: Affinity interactions, The ease and power of affinity chromatography, Attaching molecules to insoluble supports, Matrices currently in use, Over 20 methods of activation, Spacers, Extensive References

Network Intrusion Analysis Book

Network Intrusion Analysis


  • Author : Joe Fichera
  • Publisher : Newnes
  • Release Date : 2012-12-17
  • Genre: Computers
  • Pages : 252
  • ISBN 10 : 9781597499712

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Network Intrusion Analysis Book Description :

Nearly every business depends on its network to provide information services to carry out essential activities, and network intrusion attacks have been growing increasingly frequent and severe. When network intrusions do occur, it’s imperative that a thorough and systematic analysis and investigation of the attack is conducted to determine the nature of the threat and the extent of information lost, stolen, or damaged during the attack. A thorough and timely investigation and response can serve to minimize network downtime and ensure that critical business systems are maintained in full operation. Network Intrusion Analysis teaches the reader about the various tools and techniques to use during a network intrusion investigation. The book focuses on the methodology of an attack as well as the investigative methodology, challenges, and concerns. This is the first book that provides such a thorough analysis of network intrusion investigation and response. Network Intrusion Analysis addresses the entire process of investigating a network intrusion by: *Providing a step-by-step guide to the tools and techniques used in the analysis and investigation of a network intrusion. *Providing real-world examples of network intrusions, along with associated workarounds. *Walking you through the methodology and practical steps needed to conduct a thorough intrusion investigation and incident response, including a wealth of practical, hands-on tools for incident assessment and mitigation. Network Intrusion Analysis addresses the entire process of investigating a network intrusion Provides a step-by-step guide to the tools and techniques used in the analysis and investigation of a network intrusion Provides real-world examples of network intrusions, along with associated workarounds Walks readers through the methodology and practical steps needed to conduct a thorough intrusion investigation and incident response, including a wealth of practical, hands-on tools for incident assessm

Chemistry of Bioconjugates Book

Chemistry of Bioconjugates


  • Author : Ravin Narain
  • Publisher : John Wiley & Sons
  • Release Date : 2013-12-02
  • Genre: Science
  • Pages : 496
  • ISBN 10 : 9781118776377

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Chemistry of Bioconjugates Book Description :

Explores bioconjugate properties and applications ofpolymers, dendrimers, lipids, nanoparticles, and nanotubes Bioconjugation has enabled breakthroughs across many areas ofindustry and biomedicine. With its emphasis on synthesis,properties and applications, this book enables readers tounderstand the connection between chemistry and the biologicalapplication of bioconjugated materials. Its detailed descriptionsof methods make it possible for researchers to fabricate and takefull advantage of bioconjugates for a broad range of applications.Moreover, the book sets the foundation for the development of newapplications, including assays, imaging, biosensors, drug delivery,and diagnostics. Chemistry of Bioconjugates features contributions from aninternational team of leading experts and pioneers in the field.These contributions reflect the authors’ firsthand laboratoryexperience as well as a thorough review of the current literature.The book’s six sections examine: General methods of bioconjugation Polymer bioconjugates Organic nanoparticle-based bioconjugates Inorganic nanomaterial bioconjugates, including metals andmetal oxides Cell-based, hydrogel/microgel, and glyco-bioconjugates Characterization, physico-(bio)chemical properties, andapplications of bioconjugates This comprehensive exploration of bioconjugates includesdiscussions of polymers, dendrimers, lipids, nanoparticles, andnanotubes. References at the end of each chapter serve as a gatewayto the most important original research findings and reviews in thefield. By drawing together and analyzing all the latest chemicalmethods and research findings on the physico-chemical andbiochemical properties of bioconjugates, Chemistry ofBioconjugates sheds new light on the significance and potentialof bioconjugation. The book is recommended for organic and polymerchemists, biochemists, biomaterial scientists, carbohydratechemists, biophysicists, bioengineers, and drug and gene deliveryscientists.

Data Mining  Practical Machine Learning Tools and Techniques Book
Score: 5
From 2 Ratings

Data Mining Practical Machine Learning Tools and Techniques


  • Author : Ian H. Witten
  • Publisher : Elsevier
  • Release Date : 2011-02-03
  • Genre: Computers
  • Pages : 664
  • ISBN 10 : 9780080890364

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Data Mining Practical Machine Learning Tools and Techniques Book Description :

Data Mining: Practical Machine Learning Tools and Techniques, Third Edition, offers a thorough grounding in machine learning concepts as well as practical advice on applying machine learning tools and techniques in real-world data mining situations. This highly anticipated third edition of the most acclaimed work on data mining and machine learning will teach you everything you need to know about preparing inputs, interpreting outputs, evaluating results, and the algorithmic methods at the heart of successful data mining. Thorough updates reflect the technical changes and modernizations that have taken place in the field since the last edition, including new material on Data Transformations, Ensemble Learning, Massive Data Sets, Multi-instance Learning, plus a new version of the popular Weka machine learning software developed by the authors. Witten, Frank, and Hall include both tried-and-true techniques of today as well as methods at the leading edge of contemporary research. The book is targeted at information systems practitioners, programmers, consultants, developers, information technology managers, specification writers, data analysts, data modelers, database R&D professionals, data warehouse engineers, data mining professionals. The book will also be useful for professors and students of upper-level undergraduate and graduate-level data mining and machine learning courses who want to incorporate data mining as part of their data management knowledge base and expertise. Provides a thorough grounding in machine learning concepts as well as practical advice on applying the tools and techniques to your data mining projects Offers concrete tips and techniques for performance improvement that work by transforming the input or output in machine learning methods Includes downloadable Weka software toolkit, a collection of machine learning algorithms for data mining tasks—in an updated, interactive interface. Algorithms in toolkit cover: data pre-processing, classif

Placing the Suspect Behind the Keyboard Book
Score: 5
From 1 Ratings

Placing the Suspect Behind the Keyboard


  • Author : Brett Shavers
  • Publisher : Newnes
  • Release Date : 2013-02-01
  • Genre: Computers
  • Pages : 320
  • ISBN 10 : 9781597499842

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Placing the Suspect Behind the Keyboard Book Description :

Placing the Suspect Behind the Keyboard is the definitive book on conducting a complete investigation of a cybercrime using digital forensics techniques as well as physical investigative procedures. This book merges a digital analysis examiner's work with the work of a case investigator in order to build a solid case to identify and prosecute cybercriminals. Brett Shavers links traditional investigative techniques with high tech crime analysis in a manner that not only determines elements of crimes, but also places the suspect at the keyboard. This book is a first in combining investigative strategies of digital forensics analysis processes alongside physical investigative techniques in which the reader will gain a holistic approach to their current and future cybercrime investigations. Learn the tools and investigative principles of both physical and digital cybercrime investigations—and how they fit together to build a solid and complete case Master the techniques of conducting a holistic investigation that combines both digital and physical evidence to track down the "suspect behind the keyboard" The only book to combine physical and digital investigative techniques

The Essential Family Guide to Borderline Personality Disorder Book

The Essential Family Guide to Borderline Personality Disorder


  • Author : Randi Kreger
  • Publisher : Simon and Schuster
  • Release Date : 2009-06-03
  • Genre: Family & Relationships
  • Pages : 304
  • ISBN 10 : 9781592857838

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The Essential Family Guide to Borderline Personality Disorder Book Description :

Gentle counsel and realistic advice for families contending with one of today's most misunderstood forms of mental illness. "Randi Kreger has done it again! With her new book, she continues to make the dynamics of Borderline Personality Disorder (BPD) readily accessible to those of us who love, live with and treat people who suffer from this complex condition." Blaise Aguirre, MD, Medical Director, Adolescent Dialectical Behavior Therapy Residential Program, McLean Hospital"Kreger's communication techniques, grounded in the latest research, provide family members with the essential ability to regain a genuine, meaningful relationship with their loved one with Borderline Personality Disorder." Debra Resnick, Psy.D., President, Psychological Services and Human Development Center"This book offers hope for those who think their situation has none." Rachel Reiland, author of Get Me Out of Here: My Recovery from Borderline Personality DisorderFor family members of people with Borderline Personality Disorder (BPD), home life is routinely unpredictable and frequently unbearable. Extreme mood swings, impulsive behaviors, unfair blaming and criticism, and suicidal tendencies common conduct among those who suffer from the disorder leave family members feeling confused, hurt, and helpless.In Stop Walking on Eggshells, Randi Kreger's pioneering first book which sold more than 340,000 copies, she and co-author Paul T. Mason outlined the fundamental differences in the way that people with BPD relate to the world. Now, with The Essential Family Guide to Borderline Personality Disorder, Kreger takes readers to the next level by offering them five straightforward tools to organize their thinking, learn specific skills, and focus on what they need to do to get off the emotional rollercoaster:Take care of yourselfUncover what keeps you feeling stuckCommunicate to be heardSet limits with loveReinforce the right behaviorsTogether the steps provide a clear-cut system designed to help frie

Bio inspired Polymers Book

Bio inspired Polymers


  • Author : Nico Bruns
  • Publisher : Royal Society of Chemistry
  • Release Date : 2016-10-14
  • Genre: Science
  • Pages : 612
  • ISBN 10 : 9781782626664

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Bio inspired Polymers Book Description :

Many key aspects of life are based on naturally occurring polymers, such as polysaccharides, proteins and DNA. Unsurprisingly, their molecular functionalities, macromolecular structures and material properties are providing inspiration for designing new polymeric materials with specific functions, for example, responsive, adaptive and self-healing materials. Bio-inspired Polymers covers all aspects of the subject, ranging from the synthesis of novel polymers, to structure-property relationships, materials with advanced properties and applications of bio-inspired polymers in such diverse fields as drug delivery, tissue engineering, optical materials and lightweight structural materials. Written and edited by leading experts on the topic, the book provides a comprehensive review and essential graduate level text on bio-inspired polymers for biochemists, materials scientists and chemists working in both industry and academia.

15 Minute Counseling Techniques That Work Book

15 Minute Counseling Techniques That Work


  • Author : Allison Edwards
  • Publisher : 15-Minute Focus
  • Release Date : 2020-05-21
  • Genre: Education
  • Pages : 80
  • ISBN 10 : 1937870693

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15 Minute Counseling Techniques That Work Book Description :

The counselor is not the strategy. The counselor teaches strategies. As counselors, we spend our days helping kids. Kids come to us with a variety of problems, searching for answers. They want us to listen. And they need us to give them solutions for the issues they are facing. While these solutions may work temporarily, we really never help kids until we give them tools"¬‚¬"or techniques"¬‚¬"to manage thoughts and feelings on their own. Our job is not to do it for them. Our job is to teach them how to do it themselves! This is the greatest gift we can give. In 15-Minute Counseling Techniques, Allison Edwards provides tools to use in individual or group counseling sessions with children in grades K"¬‚¬"12. Children will learn how to calm their mind and body with Square Breathing, let go of negative thoughts by Changing the Channel, identify their unique gifts by creating a "What I'm Good At" Jar, and so much more. The techniques in this book will help children feel empowered to face everyday challenges and equipped to manage their stress and emotions. And, best of all, you will give them the confidence they need to handle challenges throughout their lives.

Interview Techniques for UX Practitioners Book
Score: 5
From 1 Ratings

Interview Techniques for UX Practitioners


  • Author : Chauncey Wilson
  • Publisher : Newnes
  • Release Date : 2013-11-23
  • Genre: Computers
  • Pages : 100
  • ISBN 10 : 9780124104501

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Interview Techniques for UX Practitioners Book Description :

Much of the work of user-centered design practitioners involves some type of interviewing. While interviewing is an important skill, many colleagues have little or no formal training in interviewing methods and often learn on the job with limited feedback on the quality of their interviews. This book teaches readers about the three basic interview methods: structured interviews, semi-structured interviews, and unstructured interviews. The author discusses the various strengths, weaknesses, issues with each type of interview, and includes best practices and procedures for conducing effective and efficient interviews. The book dives into the detailed information about interviews that haven’t been discussed before – readers learn how and when to ask the "how" and "why" questions to get a deeper understanding of problems, concepts, and processes, as well as discussions on laddering and critical incident techniques. Because so much of what UX practitioners do involves good interviewing skills, this is your one-stop resource with the definitions, processes, procedures and best practices on the basic approaches.

Chemical Reagents for Protein Modification  Fourth Edition Book

Chemical Reagents for Protein Modification Fourth Edition


  • Author : Roger L. Lundblad
  • Publisher : CRC Press
  • Release Date : 2014-07-22
  • Genre: Medical
  • Pages : 684
  • ISBN 10 : 9781466571914

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Chemical Reagents for Protein Modification Fourth Edition Book Description :

The use of the chemical modification of proteins has evolved over the past 80 years, benefiting from advances in analytical, physical, and organic chemistry. Over the past 30 years, the use of chemical reagents to modify proteins has been crucial in determining the function and structure of purified proteins. This groundbreaking work is part of the foundation of emerging disciplines of proteomics, chemical biology, structure biology, and chemical proteomics. Chemical Reagents for Protein Modification, Fourth Edition provides a comprehensive review of reagents used for the chemical modification of proteins, representing a major revision of the work presented in previous editions. The completely updated Fourth Edition is substantially larger and includes five new chapters: Alkylating Agents Acylating Agents Nitration and Nitrosylation Oxidation Modification of Proteins with Reducing Agents There is greatly increased coverage of the chemical modification of cysteine, which is critical for bioconjugate synthesis. The chapter on reduction also provides information necessary for bioconjugate synthesis as well as for the processing of inclusion bodies. The book places emphasis on conditions that affect the specificity of the chemical modification of proteins, such as solvent and temperature. The format has been markedly revised, presenting information based on the chemical nature of the modifying material and on the amino acid residue modified. This new version has increased significance to biopharmaceuticals. Much of the information is in tabular form, which enables the rapid location of cited material.

The Chemistry of Contrast Agents in Medical Magnetic Resonance Imaging Book

The Chemistry of Contrast Agents in Medical Magnetic Resonance Imaging


  • Author : Andre S. Merbach
  • Publisher : John Wiley & Sons
  • Release Date : 2013-02-19
  • Genre: Science
  • Pages : 512
  • ISBN 10 : 9781118503676

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The Chemistry of Contrast Agents in Medical Magnetic Resonance Imaging Book Description :

Magnetic Resonance Imaging (MRI) is one of the most important tools in clinical diagnostics and biomedical research. The number of MRI scanners operating around the world is estimated to be approximately 20,000, and the development of contrast agents, currently used in about a third of the 50 million clinical MRI examinations performed every year, has largely contributed to this significant achievement. This completely revised and extended second edition: Includes new chapters on targeted, responsive, PARACEST and nanoparticle MRI contrast agents. Covers the basic chemistries, MR physics and the most important techniques used by chemists in the characterization of MRI agents from every angle from synthesis to safety considerations. Is written for all of those involved in the development and application of contrast agents in MRI. Presented in colour, it provides readers with true representation and easy interpretation of the images. A word from the Authors: Twelve years after the first edition published, we are convinced that the chemistry of MRI agents has a bright future. By assembling all important information on the design principles and functioning of magnetic resonance imaging probes, this book intends to be a useful tool for both experts and newcomers in the field. We hope that it helps inspire further work in order to create more efficient and specific imaging probes that will allow materializing the dream of seeing even deeper and better inside the living organisms. Reviews of the First Edition: "...attempts, for the first time, to review the whole spectrum of involved chemical disciplines in this technique..."—Journal of the American Chemical Society "...well balanced in its scope and attention to detail...a valuable addition to the library of MR scientists..."—NMR in Biomedicine