Bioreactors for Stem Cell Expansion and Differentiation Book

Bioreactors for Stem Cell Expansion and Differentiation


  • Author : Joaquim M.S. Cabral
  • Publisher : CRC Press
  • Release Date : 2018-09-03
  • Genre: Medical
  • Pages : 334
  • ISBN 10 : 9780429841828

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Bioreactors for Stem Cell Expansion and Differentiation Excerpt :

An international team of investigators presents thought-provoking reviews of bioreactors for stem cell expansion and differentiation and provides cutting-edge information on different bioreactor systems. The authors offer novel insights into bioreactor-based culture systems specific for tissue engineering, including sophisticated and cost-effective manufacturing strategies geared to overcome technological shortcomings that currently preclude advances towards product commercialization. This book in the fields of stem cell expansion, bioreactors, bioprocessing, and bio and tissue engineering, gives the reader a full understanding of the state-of-art and the future of these fields. Key selling features: Describes various bioreactors or stem cell culturing systems Reviews methods for stem cell expansion and differentiation for neural, cardiac, hemopoietic, mesenchymal, hepatic and other tissues cell types Distinguishes different types of bioreactors intended for different operational scales of tissue engineering and cellular therapies Includes contributions from an international team of leaders in stem cell research

Cells and Biomaterials in Regenerative Medicine Book

Cells and Biomaterials in Regenerative Medicine


  • Author : Daniel Eberli
  • Publisher : BoD – Books on Demand
  • Release Date : 2014-11-26
  • Genre: Technology & Engineering
  • Pages : 384
  • ISBN 10 : 9789535117315

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Cells and Biomaterials in Regenerative Medicine Excerpt :

This book serves as a good starting point for anyone interested in the application of tissue engineering. It offers a colorful mix of topics, which explain the obstacles and possible solutions for TE applications. The first part covers the use of adult stem cells and their applications. The following chapters offer an insight into the development of a tailored biomaterial for organ replacement and highlight the importance of cell-biomaterial interaction. In summary, this book offers insights into a wide variety of cells, biomaterials, interfaces and applications of the next generation biotechnology, which is tissue engineering.

Development of Miniaturized Bioreactors for Stem Cell Culture Book

Development of Miniaturized Bioreactors for Stem Cell Culture


  • Author : Mariam Sarvi
  • Publisher : Unknown
  • Release Date : 2014
  • Genre: Uncategoriezed
  • Pages : 416
  • ISBN 10 : OCLC:1011512961

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Development of Miniaturized Bioreactors for Stem Cell Culture Excerpt :

Embryonic stem cells (ESCs) are pluripotent cells capable of indefinite self renewal in vitro while maintaining the ability to differentiate into cell types of all three germ layers. ESCs are outstanding options of in vitro cell models for regenerative medicine, functional genomics, human developmental biology and drug discovery study. Stem cell research is among the most promising fields of biotechnology, and which provides the potential of developing novel approaches to repair or replace damaged tissues and cells. The present day, exponentially growing effort of stem cell research emphasizes a major need for convergence of more efficient and appropriate laboratory technologies to sustain the growth, proliferation and differentiation potential of stem cells.Although so far, a variety of conventional bioreactors with different configurations (such as spinner flasks, rotary, perfusion bioreactor, etc.) have been designed and adapted for stem cell expansion and differentiation, bioreactors can be disadvantageous in bench-top research because they need large space, consume huge amount of reagents and need more time to operate and maintain (sterilizing, cleaning, assembling, and disassembling of the bioreactor components). The requirements for costly equipment and generating shear stress due to the fluid flow, and the lack of physical similarity between microenvironments of bioreactor and actual cell microenvironment, make using bioreactors undesirable.In tissue engineering, micro-scale technology is an approach that combines micro-techniques with materials science and surface engineering, and results in a profound exploration of the microenvironment where cells are embedded. These technologies are able to address some of the limitations imposed by conventional tissue engineering methods. Indeed, developing successful novel small-scale technologies for in vitro cultivation of different types of cells can assist in increasing our knowledge on conditions that control stem

Stem Cell Manufacturing Book

Stem Cell Manufacturing


  • Author : Joaquim M. S. Cabral
  • Publisher : Elsevier
  • Release Date : 2016-07-24
  • Genre: Medical
  • Pages : 340
  • ISBN 10 : 9780444632661

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Stem Cell Manufacturing Excerpt :

Stem Cell Manufacturing discusses the required technologies that enable the transfer of the current laboratory-based practice of stem cell tissue culture to the clinic environment as therapeutics, while concurrently achieving control, reproducibility, automation, validation, and safety of the process and the product. The advent of stem cell research unveiled the therapeutic potential of stem cells and their derivatives and increased the awareness of the public and scientific community for the topic. The successful manufacturing of stem cells and their derivatives is expected to have a positive impact in the society since it will contribute to widen the offer of therapeutic solutions to the patients. Fully defined cellular products can be used to restore the structure and function of damaged tissues and organs and to develop stem cell-based cellular therapies for the treatment of cancer and hematological disorders, autoimmune and other inflammatory diseases and genetic disorders. Presents the first ‘Flowchart‘ of stem cell manufacturing enabling easy understanding of the various processes in a sequential and coherent manner Covers all bioprocess technologies required for the transfer of the bench findings to the clinic including the process components: cell signals, bioreactors, modeling, automation, safety, etc. Presents comprehensive coverage of a true multidisciplinary topic by bringing together specialists in their particular area Provides the basics of the processes and identifies the issues to be resolved for large scale cell culture by the bioengineer Addresses the critical need in bioprocessing for the successful delivery of stem cell technology to the market place by involving professional engineers in sections of the book

Bioreactors for Stem Cell Expansion and Differentiation Book

Bioreactors for Stem Cell Expansion and Differentiation


  • Author : Joaquim M.S. Cabral
  • Publisher : CRC Press
  • Release Date : 2018-09-03
  • Genre: Medical
  • Pages : 334
  • ISBN 10 : 9780429841811

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Bioreactors for Stem Cell Expansion and Differentiation Excerpt :

An international team of investigators presents thought-provoking reviews of bioreactors for stem cell expansion and differentiation and provides cutting-edge information on different bioreactor systems. The authors offer novel insights into bioreactor-based culture systems specific for tissue engineering, including sophisticated and cost-effective manufacturing strategies geared to overcome technological shortcomings that currently preclude advances towards product commercialization. This book in the fields of stem cell expansion, bioreactors, bioprocessing, and bio and tissue engineering, gives the reader a full understanding of the state-of-art and the future of these fields. Key selling features: Describes various bioreactors or stem cell culturing systems Reviews methods for stem cell expansion and differentiation for neural, cardiac, hemopoietic, mesenchymal, hepatic and other tissues cell types Distinguishes different types of bioreactors intended for different operational scales of tissue engineering and cellular therapies Includes contributions from an international team of leaders in stem cell research

Embryonic Stem Cell Culture in Fibrous Bed Bioreactor Book

Embryonic Stem Cell Culture in Fibrous Bed Bioreactor


  • Author : Anli Ouyang
  • Publisher : Unknown
  • Release Date : 2006
  • Genre: Bioreactors
  • Pages : 317
  • ISBN 10 : OCLC:76805560

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Embryonic Stem Cell Culture in Fibrous Bed Bioreactor Excerpt :

Abstract: Embryonic stem (ES) cells possess unlimited self-renewal ability and the potential of differentiating to all tissue organs, making ES cells the ideal source for cell therapy and drug screening. However, present culture techniques are both expensive and labor intensive, unable to meet the projected high demand for ES cells. Three-dimensional culture is capable of mimicking in vivo growth environment and therefore, cells show their native morphology and functions. 3-D culture is superior to 2-D culture by eliminating the necessity of gelatin coating and frequent subculture. The STO cells conditioned medium is capable of replacing expensive cytokine leukemia inhibitory factor (LIF). Dynamic culture of ES cells in spinner flasks showed better cell growth and maintenance of pluripotency. Further scale up to a two stage perfusion culture system was successful, providing higher cell expansion and Oct-4 and SSEA-1 expressions. Human ES cells expansion in perfusion bioreactor also reached the success. hES cells grows faster in FBB than on 2-D surface. The hES cells pluripotency was also maintained well. However, neural differentiation efficiency was lower in 3-D culture than in 2-D system due to the higher possibility of causing de-differentiation in 3-D system. Astrocyte conditioned medium was superior to retinoic acid in inducing neural differentiation. Hydrodynamics and its influence on cell attachment and growth in 3-D cultures were also studied. Increased mixing intensity enhanced both cell attachment and detachment rates. Cell detachment kinetics can be described by Bell model. The interaction between Kolmogorov eddy size and the scaffold's pore size played a vital role in affecting shear damage to cells growing in the scaffold. When Kolmogorov eddy size is smaller than the pore size, shear damage to cells is apparent. Mathematical modeling of the fibrous bed bioreactor (FBB) was investigated too. Axial dispersion model can represent the mass transfer in orde

Stem Cell Bioprocessing Book

Stem Cell Bioprocessing


  • Author : Tiago G Fernandes
  • Publisher : Elsevier
  • Release Date : 2013-11-15
  • Genre: Science
  • Pages : 236
  • ISBN 10 : 9781908818300

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Stem Cell Bioprocessing Excerpt :

Stem cell bioprocessing describes the main large-scale bioprocessing strategies for both stem cell culture and purification, envisaging the application of these cells for regenerative medicine and drug screening. Bioreactor configurations are described, including their applications for stem cell expansion, and stem cell separation techniques such as isolation and purification are discussed. Basic definitions are provided concerning the different types of stem cells, from adult stem cells to the more recent induced pluripotent stem cells. The main characteristics of these different stem cell types are described, alongside the molecular mechanisms underlying their self-renewal and differentiation. The book also focuses on methodologies currently used for in vitro stem cell culture under static conditions, including the challenge of xeno-free culture conditions, as well as culture parameters that influence stem cell culture. Approaches for both stem cell culture and separation in micro-scale conditions are presented, including the use of cellular microarrays for high-throughput screening of the effect of both soluble and extracellular matrix molecules. A further section is dedicated to application of stem cells for regenerative medicine. Maintains a unique focus on both the basic stem cell biology concepts, and their translation to large-scale bioprocessing approaches Envisages the use of stem cells in regenerative medicine and drug screening applications Discusses the application of microscale techniques as a tool to perform basic stem cell biology studies

Expansion and Neural Differentiation of Embryonic Stem Cells in Three dimensional Cultures Book

Expansion and Neural Differentiation of Embryonic Stem Cells in Three dimensional Cultures


  • Author : Ning Liu
  • Publisher : Unknown
  • Release Date : 2010
  • Genre: Uncategoriezed
  • Pages : 202
  • ISBN 10 : OCLC:681388835

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Expansion and Neural Differentiation of Embryonic Stem Cells in Three dimensional Cultures Excerpt :

Abstract: Characterized by the unlimited self-renewal and the capability of differentiating into all cell types, embryonic stem (ES) cell is an attractive cell source for applications ranging from cell therapy, drug discovery to tissue engineering. The realization of these great applications such as the treatment of a variety of degenerative diseases including Parkinson's disease, Alzheimer's disease, heart failure and diabetes requires access to a large quantity of quality-controlled cells. However, the current culture techniques for ES cell expansion and differentiation are expensive, labor-intensive and can not meet the high demand for large numbers of stem cells needed in clinical applications. To overcome these limitations, several different culture systems, including suspension culture in spinner flasks, immobilized culture in fibrous bed bioreactors (FBB) and fibrous microcarrier culture in stirred-tank bioreactors were developed for expansion and differentiation of ES cells. By controlling the culture conditions such as the agitation rate, a suspension culture system in spinner flasks was established for ES cell expansion as aggregates. Four serial passages were studied over a culture period of 16 days in suspension culture and the system was confirmed to be reproducible. However, frequent subculturing is required to maintain the pluripotency of ES cell. Three-dimensional (3-D) culture of ES cells was studied within non-woven fibrous polyethylene terephthalate (PET) scaffolds, which not only provide a large specific surface area for cells to grow onto but also mimic more in vivo-like 3-D microenvironment, and protect cells from detrimental shear stress in bioreactors. Two different 3-D immobilized culture systems, FBB and fibrous microcarrier stirred cultures were developed using PET matrices as cell supports for ES cell cultures. It was demonstrated that the FBB culture can be extended to a longer period of 15 days without subculturing and a final high cell

New Insights into Cell Culture Technology Book

New Insights into Cell Culture Technology


  • Author : Sivakumar Joghi Thatha Gowder
  • Publisher : BoD – Books on Demand
  • Release Date : 2017-05-10
  • Genre: Science
  • Pages : 202
  • ISBN 10 : 9789535131335

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New Insights into Cell Culture Technology Excerpt :

The book "New Insights into Cell Culture Technology" focuses on many advanced methods and techniques concerned with cell culture. The contributing authors have discussed various developments in cell culture methods, the application of insect cells for the efficient production of heterologous proteins, the expansion of human mesenchymal stromal cells for different clinical applications, the remote sensing of cell culture experiments and concepts for the development of cell culture bioprocess, continuous production of retroviral pseudotype vectors, and the production of oncolytic measles virus vectors for cancer therapy. This book is an original contribution of experts from different parts of the globe, and the in-depth information will be a significant resource for students, scientists, and physicians who are directly dealing with cells.["Culture" is essential for human life and also the life of a cell. - Sivakumar Gowder]

Mesenchymal Stem Cell Therapy Book

Mesenchymal Stem Cell Therapy


  • Author : Lucas G. Chase
  • Publisher : Springer Science & Business Media
  • Release Date : 2012-12-12
  • Genre: Science
  • Pages : 456
  • ISBN 10 : 9781627032001

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Mesenchymal Stem Cell Therapy Excerpt :

Over the past decade, significant efforts have been made to develop stem cell-based therapies for difficult to treat diseases. Multipotent mesenchymal stromal cells, also referred to as mesenchymal stem cells (MSCs), appear to hold great promise in regards to a regenerative cell-based therapy for the treatment of these diseases. Currently, more than 200 clinical trials are underway worldwide exploring the use of MSCs for the treatment of a wide range of disorders including bone, cartilage and tendon damage, myocardial infarction, graft-versus-host disease, Crohn’s disease, diabetes, multiple sclerosis, critical limb ischemia and many others. MSCs were first identified by Friendenstein and colleagues as an adherent stromal cell population within the bone marrow with the ability to form clonogenic colonies in vitro. In regards to the basic biology associated with MSCs, there has been tremendous progress towards understanding this cell population’s phenotype and function from a range of tissue sources. Despite enormous progress and an overall increased understanding of MSCs at the molecular and cellular level, several critical questions remain to be answered in regards to the use of these cells in therapeutic applications. Clinically, both autologous and allogenic approaches for the transplantation of MSCs are being explored. Several of the processing steps needed for the clinical application of MSCs, including isolation from various tissues, scalable in vitro expansion, cell banking, dose preparation, quality control parameters, delivery methods and numerous others are being extensively studied. Despite a significant number of ongoing clinical trials, none of the current therapeutic approaches have, at this point, become a standard of care treatment. Although exceptionally promising, the clinical translation of MSC-based therapies is still a work in progress. The extensive number of ongoing clinical trials is expected to provide a clearer path forward for the real

Human Mesenchymal Stem Cells Book

Human Mesenchymal Stem Cells


  • Author : Mitchell Khan
  • Publisher : Nova Science Publishers
  • Release Date : 2021
  • Genre: Science
  • Pages : 134
  • ISBN 10 : 1536192244

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Human Mesenchymal Stem Cells Excerpt :

"In Chapter 1, the COVID-19 pandemic and the damage mechanisms on the cellular level which can be ameliorated with the cellular therapies is thoroughly evaluated. Previous and ongoing stem cell clinical trial data from diseases with similar symptoms is gathered. All this accumulated data and current clinical trial results indicate that the cellular therapies could be the most effective treatment option for COVID-19 patients to ameliorate the damaged tissues and save lives. In Chapter 2, the authors examine activated mesenchymal stem cells for stroke repair. Stem Cell treatment has shown recovery in animal models of stroke, indicating an improved regenerative and repair potential. Though stem cells are still being used in clinical trials, there is no evidence that they enhance recovery in ischemic stroke patients. Nevertheless, the multipotent mesenchymal stem has widely been explored for stroke recovery. An'Activated MSC' as a therapeutic alternative to tackling ischemic stroke is proposed, thereby the activation of MSCs by cytokines, growth factors, hypoxia, pharmacological drugs, etc., could be a novel approach to improving stroke patients' responses to receiving MSCs. In Chapter 3, the potential benefits of in vitro culture of therapeutic stem cells in the presence of HB along with the ketogenic diet, whereby higher physiological concentrations of ketone bodies can be achieved in vivo, as an adjuvant to stem cell transplantation is assessed"--

Stem Cells in Clinical Practice and Tissue Engineering Book

Stem Cells in Clinical Practice and Tissue Engineering


  • Author : Rakesh Sharma
  • Publisher : BoD – Books on Demand
  • Release Date : 2018-05-02
  • Genre: Science
  • Pages : 352
  • ISBN 10 : 9781789230109

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Stem Cells in Clinical Practice and Tissue Engineering Excerpt :

Stem Cells in Clinical Practice and Tissue Engineering is a concise book on applied methods of stem cell differentiation and optimization using tissue engineering methods. These methods offer immediate use in clinical regenerative medicine. The present volume will serve the purpose of applied stem cell differentiation optimization methods in clinical research projects, as well as be useful to relatively experienced stem cell scientists and clinicians who might wish to develop their stem cell clinical centers or research labs further. Chapters are arranged in the order of basic concepts of stem cell differentiation, clinical applications of pluripotent stem cells in skin, cardiac, bone, dental, obesity centers, followed by tissue engineering, new materials used, and overall evaluation with their permitted legal status.

Deferring Development Book

Deferring Development


  • Author : Cory Douglas Bishop
  • Publisher : CRC Press
  • Release Date : 2019-12-16
  • Genre: Science
  • Pages : 292
  • ISBN 10 : 9780429818110

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Deferring Development Excerpt :

This volume examines cells set aside during development for use later in ontogeny or in adult life. There is no single term for such cells. The cells explored fall within several major categories — stem cells, set-aside cells (in echinoderm larvae), imaginal discs in insects such as Drosophila, meristems (plants), blastemata (regeneration in amphibians), neoblasts (regeneration in planarians). The book compares and contrasts these cell types and the environments (niches) in which they operate with the aim of unravelling any relationships between them, between their activation in development, and in their evolution. Key Features Explores the nature of deferred-use cells in evolutionary and developmental context. Reviews the mechanisms of development of set-aside cells, such as stem cells, meristems, and imaginal discs. Provides phylogenetic overview of different types of deferred-use cells. Compares and contrasts different theories on the origin of deferred-use cells. Related Titles Calegari, F. & C. Waskow, eds. Stem Cells: From Basic Research to Therapy (ISBN 978-1-4822-0775-0) Cabral, J. M. S. & C. L. da Silva, eds. Bioreactors for Stem Cell Expansion and Differentiation (ISBN 978-1-4987-9590-6) Kong, H., A. J. Putnam, & L. B. Schook, eds. Stem Cells and Revascularization Therapies (ISBN 978-1-4398-0323-3) Schaffer, D., J. D. Bronzino, & D. R. Peterson, eds. Stem Cell Engineering: Principles and Practices (ISBN 978-1-4398-7204-8)

Cell Engineering and Regeneration Book

Cell Engineering and Regeneration


  • Author : Jeffrey M. Gimble
  • Publisher : Springer
  • Release Date : 2018-03-01
  • Genre: Technology & Engineering
  • Pages : null
  • ISBN 10 : 3319370766

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Cell Engineering and Regeneration Excerpt :

This reference work presents the origins of cells for tissue engineering and regeneration, including primary cells, tissue-specific stem cells, pluripotent stem cells and trans-differentiated or reprogrammed cells. There is particular emphasis on current understanding of tissue regeneration based on embryology and evolution studies, including mechanisms of amphibian regeneration. The book covers the use of autologous versus allogeneic cell sources, as well as various procedures used for cell isolation and cell pre-conditioning , such as cell sorting, biochemical and biophysical pre-conditioning, transfection and aggregation. It also presents cell modulation using growth factors, molecular factors, epigenetic approaches, changes in biophysical environment, cellular co-culture and other elements of the cellular microenvironment. The pathways of cell delivery are discussed with respect to specific clinical situations, including delivery of ex vivo manipulated cells via local and systemic routes, as well as activation and migration of endogenous reservoirs of reparative cells. The volume concludes with an in-depth discussion of the tracking of cells in vivo and their various regenerative activities inside the body, including differentiation, new tissue formation and actions on other cells by direct cell-to-cell communication and by secretion of biomolecules.