CRISPR and RNAi Systems Book

CRISPR and RNAi Systems


  • Author : Kamel A. Abd-Elsalam
  • Publisher : Elsevier
  • Release Date : 2021-02-27
  • Genre: Technology & Engineering
  • Pages : 840
  • ISBN 10 : 9780128219119

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CRISPR and RNAi Systems Excerpt :

Plants are vulnerable to pathogens including fungi, bacteria, and viruses, which cause critical problems and deficits. Crop protection by plant breeding delivers a promising solution with no obvious effect on human health or the local ecosystem. Crop improvement has been the most powerful approach for producing unique crop cultivars since domestication occurred, making possible the main innovations in feeding the globe and community development. Genome editing is one of the genetic devices that can be implemented, and disease resistance is frequently cited as the most encouraging application of CRISPR/Cas9 technology in agriculture. Nanobiotechnology has harnessed the power of genome editing to develop agricultural crops. Nanosized DNA or RNA nanotechnology approaches could contribute to raising the stability and performance of CRISPR guide RNAs. This book brings together the latest research in these areas. CRISPR and RNAi Systems: Nanobiotechnology Approaches to Plant Breeding and Protection presents a complete understanding of the RNAi and CRISPR/Cas9 techniques for controlling mycotoxins, fighting plant nematodes, and detecting plant pathogens. CRISPR/Cas genome editing enables efficient targeted modification in most crops, thus promising to accelerate crop improvement. CRISPR/Cas9 can be used for management of plant insects, and various plant pathogens. The book is an important reference source for both plant scientists and environmental scientists who want to understand how nano biotechnologically based approaches are being used to create more efficient plant protection and plant breeding systems. Shows how nanotechnology is being used as the basis for new solutions for more efficient plant breeding and plant protection Outlines the major techniques and applications of both CRISPR and RNAi technologies Assesses the major challenges of escalating these technologies on a mass scale

Modulating Gene Expression Book

Modulating Gene Expression


  • Author : Aditi Singh
  • Publisher : BoD – Books on Demand
  • Release Date : 2019-05-29
  • Genre: Medical
  • Pages : 136
  • ISBN 10 : 9781838806965

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Modulating Gene Expression Excerpt :

RNA interference (RNAi) is a widely used technology for gene silencing and has become a key tool in a myriad of research and lead discoveries. In recent years, the mechanism of RNAi agents has been well investigated, and the technique has been optimized for better effectiveness and safety. On the other hand, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas9/gRNA system is a recent, novel, targeted genome-editing technique derived from the bacterial immune system. Recent advances in gene-editing research and technologies have enabled the CRISPR Cas9 system to become a popular tool for sequence-specific gene editing to correct and modify eukaryotic systems. In this book, we will focus on the mechanisms, applications, regulations (their pros and cons), and various ways in which RNAi-based methods and CRIPSR-Cas9 technology have stimulated the modulation of gene expression, thereby making them a promising therapeutic tool to treat and prevent complex diseases and disorders.

RNA Interference and CRISPR Technologies Book

RNA Interference and CRISPR Technologies


  • Author : Mouldy Sioud
  • Publisher : Unknown
  • Release Date : 2020
  • Genre: Gene expression
  • Pages : 486
  • ISBN 10 : 107160290X

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RNA Interference and CRISPR Technologies Excerpt :

This volume explores the uses of RNAi and CRISPR interferences as a general method for inhibiting gene expression, with focus on their biological functions, design, chemical modifications, delivery, and preclinical/clinical applications. In addition to relevant backgrounds, the chapters in this book discuss simple and accurate protocols dealing with the latest advances in RNAi and CRISPR applications and look at how these methods differ from one another. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, RNA Interference and CRISPR Technologies: Technical Advances and New Therapeutic Opportunities is a valuable resource for any scientist, teachers, graduate student, postdoc, and clinician interested in this field. This book also benefits anyone in research and development in biotech and pharmaceutical companies who want to understand more about these technologies, and their applications in biology and medicine. .

CRISPR Cas Systems Book

CRISPR Cas Systems


  • Author : Rodolphe Barrangou
  • Publisher : Springer Science & Business Media
  • Release Date : 2012-12-13
  • Genre: Science
  • Pages : 302
  • ISBN 10 : 9783642346576

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CRISPR Cas Systems Excerpt :

CRISPR/Cas is a recently described defense system that protects bacteria and archaea against invasion by mobile genetic elements such as viruses and plasmids. A wide spectrum of distinct CRISPR/Cas systems has been identified in at least half of the available prokaryotic genomes. On-going structural and functional analyses have resulted in a far greater insight into the functions and possible applications of these systems, although many secrets remain to be discovered. In this book, experts summarize the state of the art in this exciting field.

RNAi Technology Book

RNAi Technology


  • Author : R. K. Gaur
  • Publisher : CRC Press
  • Release Date : 2016-04-19
  • Genre: Science
  • Pages : 380
  • ISBN 10 : 9781439873250

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RNAi Technology Excerpt :

RNAi technology is used for large-scale screens that systematically shut down each gene in the cell, which can help identify the components necessary for a particular cellular process or an event such as cell division. Exploitation of the pathway is also a promising tool in biotechnology and medicine. Introducing new technology in the study of RNA

Epigenetics in Health and Disease Book

Epigenetics in Health and Disease


  • Author : Igor Kovalchuk
  • Publisher : FT Press
  • Release Date : 2012-05-22
  • Genre: Education
  • Pages : 608
  • ISBN 10 : 9780132599511

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Epigenetics in Health and Disease Excerpt :

This is the first comprehensive, authoritative, and easy-to-understand introduction to modern epigenetics. Authored by two active researchers in the field, it introduces key concepts one step at a time, enabling students at all levels to benefit from it. The authors begin by presenting a historical overview that places epigenetics in context, and makes it clear that the field is not (as some presume) completely new. Next, they introduce and explain key epigenetic mechanisms, and discuss the roles these mechanisms may play in inheritance, organism development, health and disease, behavior, evolution, ecology, and the interaction of individual organisms with their environments. Coverage includes: non-coding RNAs in each kingdom; allelic interactions; CRYSPR; gene silencing; epigenetics of germline and epigenetic memory; epigenetic regulation of genome stability and plant stress response; and much more. The authors conclude by offering significant new insights into how knowledge of epigenetics and epigenomics may promote the development of technologies and solutions in areas ranging from behavioral neuroscience to cancer treatment, toxicology to the development of hardier crops.

Advances in Nucleic Acid Therapeutics Book

Advances in Nucleic Acid Therapeutics


  • Author : Sudhir Agrawal
  • Publisher : Royal Society of Chemistry
  • Release Date : 2019-02-08
  • Genre: Science
  • Pages : 541
  • ISBN 10 : 9781788017329

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Advances in Nucleic Acid Therapeutics Excerpt :

The sequencing of the human genome and subsequent elucidation of the molecular pathways that are important in the pathology of disease have provided unprecedented opportunities for the development of new therapeutics. Nucleic acid-based drugs have emerged in recent years to yield extremely promising candidates for drug therapy to a wide range of diseases. Advances in Nucleic Acid Therapeutics is a comprehensive review of the latest advances in the field, covering the background of the development of nucleic acids for therapeutic purposes to the array of drug development approaches currently being pursued using antisense, RNAi, aptamer, immune modulatory and other synthetic oligonucleotides. Nucleic acid therapeutics is a field that has been continually innovating to meet the challenges of drug discovery and development; bringing contributions together from leaders at the forefront of progress, this book depicts the many approaches currently being pursued in both academia and industry. A go-to volume for medicinal chemists, Advances in Nucleic Acid Therapeutics provides a broad overview of techniques of contemporary interest in drug discovery.

The CRISPR Cas Tool Kit for Genome Editing Book

The CRISPR Cas Tool Kit for Genome Editing


  • Author : Aftab Ahmad (Biochemist)
  • Publisher : Springer Nature
  • Release Date : 2021
  • Genre: Biotechnology
  • Pages : 342
  • ISBN 10 : 9789811663055

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The CRISPR Cas Tool Kit for Genome Editing Excerpt :

This book discusses CRISPR/Cas- one of the most powerful tools available to scientists for genome editing. CRISPR/Cas is not only a genome editing tool, but researchers have also engineered it for gene regulation, genome imaging, base editing and epigenome regulations. This book describes the entire toolkit for CRISPR/Cas. The opening section gives an introduction to the technique and compares it with other genome editing tools. Further section gives a historical perspective of the tool, along with its detailed classification. The next chapters describe bioinformatic tools in CRISPR/Cas, and delivery methods for CRISPR/Cas. The book also discusses about the applications of CRISPR/Cas beyond genome editing and use of CRISPR for rewriting genetic codes. The book dedicates a section to the use of CRISPR in plants. The book culminates with a chapter on the current status, challenges and shortcomings of the CRISPR/Cas genome editing tool. The book would be highly interesting to students and researchers in molecular biology, biochemistry, biotechnology, food science, agriculture and plant sciences.

Biotechnologies of Crop Improvement  Volume 2 Book

Biotechnologies of Crop Improvement Volume 2


  • Author : Satbir Singh Gosal
  • Publisher : Springer
  • Release Date : 2018-07-09
  • Genre: Science
  • Pages : 485
  • ISBN 10 : 9783319906508

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Biotechnologies of Crop Improvement Volume 2 Excerpt :

During the past 15 years, cellular and molecular approaches have emerged as valuable adjuncts to supplement and complement conventional breeding methods for a wide variety of crop plants. Biotechnology increasingly plays a role in the creation, conservation, characterization and utilization of genetic variability for germplasm enhancement. For instance, anther/microspore culture, somaclonal variation, embryo culture and somatic hybridization are being exploited for obtaining incremental improvement in the existing cultivars. In addition, genes that confer insect- and disease-resistance, abiotic stress tolerance, herbicide tolerance and quality traits have been isolated and re-introduced into otherwise sensitive or susceptible species by a variety of transgenic techniques. Together these transformative methodologies grant access to a greater repertoire of genetic diversity as the gene(s) may come from viruses, bacteria, fungi, insects, animals, human beings, unrelated plants or even be artificially derived. Remarkable achievements have been made in the production, characterization, field evaluation and commercialization of transgenic crop varieties worldwide. Likewise, significant advances have been made towards increasing crop yields, improving nutritional quality, enabling crops to be raised under adverse conditions and developing resistance to pests and diseases for sustaining global food and nutritional security. The overarching purpose of this 3-volume work is to summarize the history of crop improvement from a technological perspective but to do so with a forward outlook on further advancement and adaptability to a changing world. Our carefully chosen “case studies of important plant crops” intend to serve a diverse spectrum of audience looking for the right tools to tackle complicated local and global issues.

Crop Improvement for Sustainable Agriculture Book

Crop Improvement for Sustainable Agriculture


  • Author : Mitchell Brett Callaway
  • Publisher : U of Nebraska Press
  • Release Date : 1993-01-01
  • Genre: Science
  • Pages : 286
  • ISBN 10 : 0803214626

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Crop Improvement for Sustainable Agriculture Excerpt :

Crop improvement for future farming systems. History of crop improvement in sustainable agriculture. Choosing germplasm for breeding program success. The role of seed companies in crop improvement. Crop breeding objectives and methods. Breeding for resistance to insects and plant pathogens. Crop tolerance to weeds. Tree improvement for agroforestry systems. Contributions of biotechnology to crop improvement. Genotype by environment interaction in crop improvement. Statistical design and analysis of intercropping experiments.

RNA Interference Book

RNA Interference


  • Author : Ibrokhim Y. Abdurakhmonov
  • Publisher : BoD – Books on Demand
  • Release Date : 2016-04-06
  • Genre: Medical
  • Pages : 460
  • ISBN 10 : 9789535122722

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RNA Interference Excerpt :

RNA interference (RNAi), a hallmark of all biological sciences of twenty-first century, is an evolutionarily conserved and double-stranded RNA-dependent eukaryotic cell defense process. Opportunity to utilize an organisms own gene and to systematically induce and trigger RNAi for any desired sequence made RNAi an efficient approach for functional genomics, providing a solution for conventional longstanding obstacles in life sciences. RNAi research and application have significantly advanced during past two decades. This book RNA interference provides an updated knowledge and progress on RNAi in various organisms, explaining basic principles, types, and property of inducers, structural modifications, delivery systems/methodologies, and various successful bench-to-field or clinic applications and disease therapies with some aspects of limitations, alternative tools, safety, and risk assessment.

Genome Editing in Neurosciences Book

Genome Editing in Neurosciences


  • Author : Rudolf Jaenisch
  • Publisher : Unknown
  • Release Date : 2020-10-08
  • Genre: Medical
  • Pages : 128
  • ISBN 10 : 1013268563

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Genome Editing in Neurosciences Excerpt :

Innovations in molecular biology are allowing neuroscientists to study the brain with unprecedented resolution, from the level of single molecules to integrated gene circuits. Chief among these innovations is the CRISPR-Cas genome editing technology, which has the precision and scalability to tackle the complexity of the brain. This Colloque Médecine et Recherche has brought together experts from around the world that are applying genome editing to address important challenges in neuroscience, including basic biology in model organisms that has the power to reveal systems-level insight into how the nervous system develops and functions as well as research focused on understanding and treating human neurological disorders. This work was published by Saint Philip Street Press pursuant to a Creative Commons license permitting commercial use. All rights not granted by the work's license are retained by the author or authors.

Rice Improvement Book

Rice Improvement


  • Author : Jauhar Ali
  • Publisher : Springer Nature
  • Release Date : 2021-05-05
  • Genre: Technology & Engineering
  • Pages : 498
  • ISBN 10 : 9783030665302

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Rice Improvement Excerpt :

This book is open access under a CC BY 4.0 license. By 2050, human population is expected to reach 9.7 billion. The demand for increased food production needs to be met from ever reducing resources of land, water and other environmental constraints. Rice remains the staple food source for a majority of the global populations, but especially in Asia where ninety percent of rice is grown and consumed. Climate change continues to impose abiotic and biotic stresses that curtail rice quality and yields. Researchers have been challenged to provide innovative solutions to maintain, or even increase, rice production. Amongst them, the ‘green super rice’ breeding strategy has been successful for leading the development and release of multiple abiotic and biotic stress tolerant rice varieties. Recent advances in plant molecular biology and biotechnologies have led to the identification of stress responsive genes and signaling pathways, which open up new paradigms to augment rice productivity. Accordingly, transcription factors, protein kinases and enzymes for generating protective metabolites and proteins all contribute to an intricate network of events that guard and maintain cellular integrity. In addition, various quantitative trait loci associated with elevated stress tolerance have been cloned, resulting in the detection of novel genes for biotic and abiotic stress resistance. Mechanistic understanding of the genetic basis of traits, such as N and P use, is allowing rice researchers to engineer nutrient-efficient rice varieties, which would result in higher yields with lower inputs. Likewise, the research in micronutrients biosynthesis opens doors to genetic engineering of metabolic pathways to enhance micronutrients production. With third generation sequencing techniques on the horizon, exciting progress can be expected to vastly improve molecular markers for gene-trait associations forecast with increasing accuracy. This book emphasizes on the areas of rice science

RNA Nanotechnology Book

RNA Nanotechnology


  • Author : Bin Wang
  • Publisher : CRC Press
  • Release Date : 2014-04-02
  • Genre: Medical
  • Pages : 468
  • ISBN 10 : 9789814411646

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RNA Nanotechnology Excerpt :

In the past few decades there has been incredible growth in "bionano"-related research, which has been accompanied by numerous publications in this field. Although various compilations address topics related to deoxyribonucleic acid (DNA) and protein, there are few books that focus on determining the structure of ribonucleic acid (RNA) and using RNA as building blocks to construct nanoarchitectures for biomedical and healthcare applications. RNA Nanotechnology is a comprehensive volume that details both the traditional approaches and the latest developments in the field of RNA-related technology. This book targets a wide audience: a broad introduction provides a solid academic background for students, researchers, and scientists who are unfamiliar with the subject, while the in-depth descriptions and discussions are useful for advanced professionals. The book opens with reviews on the basic aspects of RNA biology, computational approaches for predicting RNA structures, and traditional and emerging experimental approaches for probing RNA structures. This section is followed by explorations of the latest research and discoveries in RNA nanotechnology, including the design and construction of RNA-based nanostructures. The final segment of the book includes descriptions and discussions of the potential biological and therapeutic applications of small RNA molecules, such as small/short interfering RNAs (siRNAs), microRNAs (miRNAs), RNA aptamers, and ribozymes.