Fluorescence Microscopy Book

Fluorescence Microscopy


  • Author : Ulrich Kubitscheck
  • Publisher : John Wiley & Sons
  • Release Date : 2017-03-27
  • Genre: Medical
  • Pages : 496
  • ISBN 10 : 9783527687725

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Fluorescence Microscopy Excerpt :

While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques. This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy as well as an expanded introduction. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to reflect the developments in recent years.

Fluorescence Microscopy Book

Fluorescence Microscopy


  • Author : Anda Cornea
  • Publisher : Elsevier
  • Release Date : 2014-02-24
  • Genre: Science
  • Pages : 260
  • ISBN 10 : 9780124167131

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Fluorescence Microscopy Excerpt :

Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers. With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, Fluorescence Microscopy encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research. Presents a timely and comprehensive review of novel techniques in fluorescence imaging as applied to biological and biomedical research Offers insight into common challenges in implementing techniques, as well as effective solutions

Advanced Fluorescence Microscopy Book

Advanced Fluorescence Microscopy


  • Author : Peter J. Verveer
  • Publisher : Humana Press
  • Release Date : 2014-11-13
  • Genre: Medical
  • Pages : 294
  • ISBN 10 : 1493920790

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Advanced Fluorescence Microscopy Excerpt :

This volume provides an overview of advanced fluorescence microscopy, covering a broad range of methods. Each chapter focuses on a different method and provides a practical guide for application in biological systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Advanced Fluorescence Microscopy: Methods and Protocols seeks to provide scientists with methods for biological systems that are of interest.

Fundamentals of Fluorescence Microscopy Book

Fundamentals of Fluorescence Microscopy


  • Author : Partha Pratim Mondal
  • Publisher : Springer Science & Business Media
  • Release Date : 2013-12-12
  • Genre: Science
  • Pages : 218
  • ISBN 10 : 9789400775459

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Fundamentals of Fluorescence Microscopy Excerpt :

This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.

Introduction to Fluorescence Microscopy Book

Introduction to Fluorescence Microscopy


  • Author : J. S. Ploem
  • Publisher : Garland Science
  • Release Date : 1987
  • Genre: Juvenile Nonfiction
  • Pages : 56
  • ISBN 10 : UOM:39015012610625

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Introduction to Fluorescence Microscopy Excerpt :

Covers three main areas.The phenomenon of fluorescence, the main applications, and the most common problems.

Quantitative Fluorescence Microscopy Book

Quantitative Fluorescence Microscopy


  • Author : F. W. D. Rost
  • Publisher : Cambridge University Press
  • Release Date : 1991-07-26
  • Genre: Medical
  • Pages : 264
  • ISBN 10 : 0521394228

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Quantitative Fluorescence Microscopy Excerpt :

This book is a complete guide to the technique of fluorescence microscopy. It describes the history, principles, and applications of quantitative fluorescence microscopy and also gives much practical information about the instrumentation required. In addition, there is a discussion of the exciting developments in confocal fluorescence microscopy, which allows the three-dimensional distribution of particular substances to be determined.

Fluorescence Microscopy Book

Fluorescence Microscopy


  • Author : F. W. D. Rost
  • Publisher : Cambridge University Press
  • Release Date : 1992
  • Genre: Science
  • Pages : 486
  • ISBN 10 : 0521410886

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Fluorescence Microscopy Excerpt :

Fluorescence microscopy is used for studying the distribution of substances which are present in very small amounts, for example in living cells. This magnificent new work provides comprehensive cover of all aspects of fluorescence microscopy - including instrumentation, applications, and the history of the technique. The first volume deals with instrumentation and techniques for fluorescence microscopy, and includes a chapter on quantification and scanning. The second volume deals with the applications of fluorescence microscopy in many fields. It includes information on autofluorescence, and an invaluable appendix provides an alphabetical list of fluorochromes, giving information concerning chemical structure, fluorescence properties, applications and suitable filter combinations.

Introduction to Confocal Fluorescence Microscopy Book
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Introduction to Confocal Fluorescence Microscopy


  • Author : Michiel Muller
  • Publisher : SPIE Press
  • Release Date : 2006
  • Genre: Science
  • Pages : 142
  • ISBN 10 : 0819460435

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Introduction to Confocal Fluorescence Microscopy Excerpt :

This book provides a comprehensive account of the theory of image formation in a confocal fluorescence microscope as well as a practical guideline to the operation of the instrument, its limitations, and the interpretation of confocal microscopy data. The appendices provide a quick reference to optical theory, microscopy-related formulas and definitions, and Fourier theory.

Optical Fluorescence Microscopy Book

Optical Fluorescence Microscopy


  • Author : Alberto Diaspro
  • Publisher : Springer Science & Business Media
  • Release Date : 2010-11-25
  • Genre: Science
  • Pages : 252
  • ISBN 10 : 9783642151750

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Optical Fluorescence Microscopy Excerpt :

In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.

Protein Localization by Fluorescence Microscopy Book

Protein Localization by Fluorescence Microscopy


  • Author : Viki Allan
  • Publisher : OUP Oxford
  • Release Date : 1999-11-18
  • Genre: Science
  • Pages : 256
  • ISBN 10 : 9780191565915

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Protein Localization by Fluorescence Microscopy Excerpt :

There is an ever-increasing number of genes that have been sequenced but are of completely unknown function. The ability to determine the location of such gene products within the cell, either by the use of antibodies or by the production of chimeras with green fluorescent protein, is a vital step towards understanding what they do. This is one major reason why fluorescence microscopy is enjoying a revival. This no-nonsense guide provides detailed, practical advice on all aspects of the subject: from choosing the right equipment, to interpreting results. It balances the advantages of a wide range of techniques - including live cell work - against the potential pitfalls, offering invaluable "tricks of the trade" along the way. Protein Localization by Fluorescence Light Microscopy: A Practical Approach has something to offer all microscopists, giving a solid grounding to the novice whilst extending the range of the experienced user.

Fluorescence Microscopy and Fluorescent Probes Book

Fluorescence Microscopy and Fluorescent Probes


  • Author : J. Slavík
  • Publisher : Springer Science & Business Media
  • Release Date : 2013-11-11
  • Genre: Science
  • Pages : 306
  • ISBN 10 : 9781489918666

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Fluorescence Microscopy and Fluorescent Probes Excerpt :

Fluorescence microscopy images can be easily integrated into current video and computer image processing systems. People like visual observation; they like to watch a television or computer screen, and fluorescence techniques are thus becoming more and more popular. Since true in vivo experiments are simple to perform, samples can be directly seen and there is always the possibility of manipulating the samples during the experiments; it is an ideal technique for biology and medicine. Images are obtained by a classical (now called wide-field) fluorescence microscope, a confocal scanning microscope, upright or inverted, with epifluorescence or transmission. Computerized image processing may improve definition, and remove glare and scattered light signal. It also makes it possible to compute ratio images (ratio imaging both in excitation and in emission) or lifetime imaging. Image analysis programs may supply a great deal of additional data of various types, starting with calculations of the number of fluorescent objects, their shapes, brightness, etc. Fluorescence microscopy data may be complemented by classical measurement in the cuvette yr by flow cytometry.

Fluorescence Microscopy and Fluorescent Probes Book

Fluorescence Microscopy and Fluorescent Probes


  • Author : Jan Slavík
  • Publisher : Springer Science & Business Media
  • Release Date : 1998-08-31
  • Genre: Science
  • Pages : 298
  • ISBN 10 : 0306460211

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Fluorescence Microscopy and Fluorescent Probes Excerpt :

The April 1997 conference held in Prague attracted the cream of primarily European and Russian researchers (with a handful from the US, primarily from the U. of Maryland School of Medicine) to the burgeoning biological and medical applications of innovative optical technology, particularly laser confocal fluorescence microscopy. Six invited review lectures illuminate these recent developments and their role in cell biology, including computer simulations of molecular behavior. The 34 other papers focus on the major themes of: new developments in fluorescence instrumentation; fluorescent probes; nucleic acid labels; and other fluorescent labels, markers, and fluorogenic substrates. Annotation copyrighted by Book News, Inc., Portland, OR

Nanoscopy and Multidimensional Optical Fluorescence Microscopy Book

Nanoscopy and Multidimensional Optical Fluorescence Microscopy


  • Author : Alberto Diaspro
  • Publisher : CRC Press
  • Release Date : 2010-04-26
  • Genre: Technology & Engineering
  • Pages : 448
  • ISBN 10 : 9781420078893

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Nanoscopy and Multidimensional Optical Fluorescence Microscopy Excerpt :

"Alberto Diaspro has been choreographing light’s dance for over 20 years, and in Nanoscopy and Multidimensional Optical Fluorescence Microscopy, he has assembled a diverse group of experts to explain the methods they use to coax light to reveal biology’s secrets." — From the Foreword by Daniel Evanko, editor, Nature Methods Nanoscopy and Multidimensional Optical Fluorescence Microscopy demonstrates that the boundaries between sciences do blur at the bottom, especially those that might separate the optical work of physicists and the cellular work of microbiologists. In 18 chapters written by pioneering researchers, this work offers the first comprehensive and current documentation of the cutting-edge research being accomplished in a wide range of photonic devices with revolutionary application. The highlight of the book is its coverage of optical nanoscopy and super-resolution microscopy. The rapid advances in this area over the past few years offer researchers in both photonics and molecular biologya wealth of accomplishment upon which they can build. Offering a complete treatment of this emerging field, this volume: Describes how scientists have exploited the properties of light and its fluorophore partners to overcome the resolution limit of conventional light microscopy Delves into recent ways to minimize the photobleaching that has long hampered many methods including those that have the potential to capture previously unobtainable information on the movements of single molecules Discusses the principles, benefits, and implementation of fluorescence correlation spectroscopy and related methods, which simplifies analysis by limiting light to stationary focal points in a sample Considers the most basic as well as emerging methods for improving three-dimensional optical sectioning microscopy Reviews the basics of FRET (fluorescence resonance energy transfer) and considers its new use for investigating protein complexes The text also introduces those emerging