Handbook of Single Molecule Biophysics Book

Handbook of Single Molecule Biophysics


  • Author : Peter Hinterdorfer
  • Publisher : Springer Science & Business Media
  • Release Date : 2009-12-24
  • Genre: Science
  • Pages : 626
  • ISBN 10 : 0387764976

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Handbook of Single Molecule Biophysics Excerpt :

This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.

Single Molecule Biophysics and Poisson Process Approach to Statistical Mechanics Book

Single Molecule Biophysics and Poisson Process Approach to Statistical Mechanics


  • Author : Susanta K Sarkar
  • Publisher : Morgan & Claypool Publishers
  • Release Date : 2016-04-01
  • Genre: Science
  • Pages : 74
  • ISBN 10 : 9781681741802

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Single Molecule Biophysics and Poisson Process Approach to Statistical Mechanics Excerpt :

This is an overview of single molecule physics, the study of both equilibrium and non-equilibrium properties at the single molecule level. It begins with an introduction to this fascinating science and includes a chapter on how to build the most popular instrument for single molecule biophysics, the total internal reflection fluorescence (TIRF) microscope. It concludes with the Poisson process approach to statistical mechanics, explaining how to relate the process to diverse areas and see how data analysis and error bars are integral parts of science.

An Introduction to Single Molecule Biophysics Book

An Introduction to Single Molecule Biophysics


  • Author : Yuri L. Lyubchenko
  • Publisher : CRC Press
  • Release Date : 2017-11-22
  • Genre: Science
  • Pages : 240
  • ISBN 10 : 9781351333122

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An Introduction to Single Molecule Biophysics Excerpt :

This book gives an accessible, detailed overview on techniques of single molecule biophysics (SMB), showing how they are applied to numerous biological problems associated with understanding the molecular mechanisms of DNA replication, transcription, and translation, as well as functioning of molecular machines. It covers major single molecule imaging and probing techniques, highlighting key strengths and limitations of each method using recent examples. The chapters begin with a discussion of single molecule fluorescence techniques followed by an overview of the atomic force microscope and its use for direct time-lapse visualization of dynamics of molecular complexes at the nanoscale, as well as applications in measurements of interactions between molecules and mechanical properties of isolated molecules and their complexes. The next chapters address magnetic tweezers and optical tweezers, including instrumentation, fundamentals of operation, and applications. A final chapter turns to nanopore transport and nanopore-based DNA sequencing technology that will play a major role in next-generation genomics and healthcare applications.

Single Molecule Science Book

Single Molecule Science


  • Author : Krishnarao Appasani
  • Publisher : Cambridge University Press
  • Release Date : 2022-05-31
  • Genre: Medical
  • Pages : 550
  • ISBN 10 : 9781108423366

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Single Molecule Science Excerpt :

A comprehensive volume that brings together authoritative overviews of single molecule science techniques from a biological perspective.

Single Molecule Tools for Bioanalysis Book

Single Molecule Tools for Bioanalysis


  • Author : Shuo Huang
  • Publisher : CRC Press
  • Release Date : 2022-05-26
  • Genre: Medical
  • Pages : 278
  • ISBN 10 : 9781000409215

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Single Molecule Tools for Bioanalysis Excerpt :

In the last three decades, the fast development of single-molecule techniques has revolutionized the way we observe and understand biological processes. Some of these techniques have been further adapted as tools for bioanalysis. This book summarizes and details the frontiers of the development of these tools as well as their applications. The contributors are young and established researchers in their respective fields. The main content originates from the lecture notes of a chemistry graduate course taught by the book editor at Nanjing University. This book is suitable to be used as a textbook for a high-level undergraduate or an entry-level graduate course. The systematically written content provides a thorough illustration of the mechanisms of each methodology presented.

Handbook of Molecular Force Spectroscopy Book

Handbook of Molecular Force Spectroscopy


  • Author : Aleksandr Noy
  • Publisher : Springer Science & Business Media
  • Release Date : 2007-12-03
  • Genre: Science
  • Pages : 300
  • ISBN 10 : 9780387499895

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Handbook of Molecular Force Spectroscopy Excerpt :

Researchers in academia and industry who are interested in techniques for measuring intermolecular forces will find this an essential text. It presents a review of modern force spectroscopy, including fundamentals of intermolecular forces, technical aspects of the force measurements, and practical applications. The handbook begins with a review of the fundamental physics of loading single and multiple chemical bonds on the nanometer scale. It contains a discussion of thermodynamic and kinetic models of binding forces and dissipation effects in nanoscale molecular contacts, covers practical aspects of modern single-molecule level techniques, and concludes with applications of force spectroscopy to chemical and biological processes. Computer modeling of force spectroscopy experiments is also addressed.

Handbook of Fluorescence Spectroscopy and Imaging Book
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Handbook of Fluorescence Spectroscopy and Imaging


  • Author : Markus Sauer
  • Publisher : John Wiley & Sons
  • Release Date : 2010-12-23
  • Genre: Science
  • Pages : 290
  • ISBN 10 : 9783527633524

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Handbook of Fluorescence Spectroscopy and Imaging Excerpt :

Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.

Handbook of Single Molecule Fluorescence Spectroscopy Book

Handbook of Single Molecule Fluorescence Spectroscopy


  • Author : Chris Gell
  • Publisher : OUP Oxford
  • Release Date : 2006-08-17
  • Genre: Science
  • Pages : 278
  • ISBN 10 : 9780191523854

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Handbook of Single Molecule Fluorescence Spectroscopy Excerpt :

Analytical measurements at the single molecule level under ambient conditions have become almost routine in the past few years. The application of this technology to fundamental studies of heterogeneity in biomolecular structure and dynamics, chemical and biological reaction kinetics, and photophysics provides a rich playground for molecular scientists. The potential use of single molecule detection for nanotechnology and quantum information processing is a new and almost unexplored area. This handbook is intended for those interested in a practical introduction to single molecule investigations using fluorescence techniques and places special emphasis on the practicalities of achieving single molecule resolution, analysing the resulting data and exploration of the applications in biophysics. It is ideal for graduate research students and others embarking on work in this exciting field.

Handbook of Single Molecule Fluorescence Spectroscopy Book

Handbook of Single Molecule Fluorescence Spectroscopy


  • Author : Chris Gell
  • Publisher : Oxford University Press on Demand
  • Release Date : 2006-08-17
  • Genre: Science
  • Pages : 262
  • ISBN 10 : 9780198529422

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Handbook of Single Molecule Fluorescence Spectroscopy Excerpt :

This is a practical introduction to single molecule fluorescence experiments, the analysis of the data, and applications of the techniques to the study of biological structure and function.

Single Molecule Enzymology  Fluorescence Based and High Throughput Methods Book

Single Molecule Enzymology Fluorescence Based and High Throughput Methods


  • Author : Anonim
  • Publisher : Academic Press
  • Release Date : 2016-10-28
  • Genre: Medical
  • Pages : 616
  • ISBN 10 : 9780128095478

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Single Molecule Enzymology Fluorescence Based and High Throughput Methods Excerpt :

Single-Molecule Enzymology, Part A, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in single-molecule enzymology Contains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion

Advances in Molecular Biophotonics Book

Advances in Molecular Biophotonics


  • Author : Yong Deng
  • Publisher : Walter de Gruyter GmbH & Co KG
  • Release Date : 2017-04-24
  • Genre: Science
  • Pages : 416
  • ISBN 10 : 9783110304596

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Advances in Molecular Biophotonics Excerpt :

Being the most active field in modern physics, Optical Physics has developed many new branches and interdisciplinary fields overlapping with various classical disciplines. This series summarizes the advancements of optical physics in the past twenty years in the following fields: High Field Laser Physics, Precision Laser Spectroscopy, Nonlinear Optics, Nanophotonics, Quantum Optics, Ultrafast Optics, Condensed Matter Optics, and Molecular Biophotonics.

Cellular Mechanics and Biophysics Book

Cellular Mechanics and Biophysics


  • Author : Claudia Tanja Mierke
  • Publisher : Springer Nature
  • Release Date : 2020-10-30
  • Genre: Science
  • Pages : 900
  • ISBN 10 : 9783030585327

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Cellular Mechanics and Biophysics Excerpt :

This book focuses on the mechanical properties of cells, discussing the basic concepts and processes in the fields of immunology, biology, and biochemistry. It introduces and explains state-of-the-art biophysical methods and examines the role of mechanical properties in the cell/protein interaction with the connective tissue microenvironment. The book presents a unique perspective on cellular mechanics and biophysics by combining the mechanical, biological, physical, biochemical, medical, and immunological views, highlighting the importance of the mechanical properties of cells and biophysical measurement methods. The book guides readers through the complex and growing field of cellular mechanics and biophysics, connecting and discussing research findings from different fields such as biology, cell biology, immunology, physics, and medicine. Featuring suggestions for further reading throughout and addressing a wide selection of biophysical topics, this book is an indispensable guide for graduate and advanced undergraduate students in the fields of cellular mechanics and biophysics.

Handbook of Clinical Nanomedicine Book

Handbook of Clinical Nanomedicine


  • Author : Raj Bawa
  • Publisher : CRC Press
  • Release Date : 2016-02-22
  • Genre: Medical
  • Pages : 1708
  • ISBN 10 : 9789814669214

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Handbook of Clinical Nanomedicine Excerpt :

This handbook (55 chapters) provides a comprehensive roadmap of basic research in nanomedicine as well as clinical applications. However, unlike other texts in nanomedicine, it not only highlights current advances in diagnostics and therapeutics but also explores related issues like nomenclature, historical developments, regulatory aspects, nanosim

Biomolecular Kinetics Book

Biomolecular Kinetics


  • Author : Clive R. Bagshaw
  • Publisher : CRC Press
  • Release Date : 2017-10-04
  • Genre: Medical
  • Pages : 446
  • ISBN 10 : 9781498727242

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Biomolecular Kinetics Excerpt :

"a gem of a textbook which manages to produce a genuinely fresh, concise yet comprehensive guide" –Mark Leake, University of York "destined to become a standard reference.... Not just a ‘how to’ handbook but also an accessible primer in the essentials of kinetic theory and practice." –Michael Geeves, University of Kent "covers the entire spectrum of approaches, from the traditional steady state methods to a thorough account of transient kinetics and rapid reaction techniques, and then on to the new single molecule techniques" –Stephen Halford, University of Bristol This illustrated treatment explains the methods used for measuring how much a reaction gets speeded up, as well as the framework for solving problems such as ligand binding and macromolecular folding, using the step-by-step approach of numerical integration. It is a thoroughly modern text, reflecting the recent ability to observe reactions at the single-molecule level, as well as advances in microfluidics which have given rise to femtoscale studies. Kinetics is more important now than ever, and this book is a vibrant and approachable entry for anyone who wants to understand mechanism using transient or single molecule kinetics without getting bogged down in advanced mathematics. Clive R. Bagshaw is Emeritus Professor at the University of Leicester, U.K., and Research Associate at the University of California at Santa Cruz, U.S.A.

Single Molecule Cellular Biophysics Book

Single Molecule Cellular Biophysics


  • Author : Mark C. Leake
  • Publisher : Cambridge University Press
  • Release Date : 2013-01-31
  • Genre: Science
  • Pages : null
  • ISBN 10 : 9781139619349

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Single Molecule Cellular Biophysics Excerpt :

Recent advances in single molecule science have presented a new branch of science: single molecule cellular biophysics, combining classical cell biology with cutting-edge single molecule biophysics. This textbook explains the essential elements of this new discipline, from the state-of-the-art single molecule techniques to real-world applications in unravelling the inner workings of the cell. Every effort has been made to ensure the text can be easily understood by students from both the physical and life sciences. Mathematical derivations are kept to a minimum whilst unnecessary biological terminology is avoided and text boxes provide readers from either background with additional information. 100 end-of-chapter exercises are divided into those aimed at physical sciences students, those aimed at life science students and those that can be tackled by students from both disciplines. The use of case studies and real research examples make this textbook indispensable for undergraduate students entering this exciting field.