In Situ Molecular Pathology and Co Expression Analyses Book

In Situ Molecular Pathology and Co Expression Analyses


  • Author : Gerard J. Nuovo
  • Publisher : Academic Press
  • Release Date : 2013
  • Genre: Medical
  • Pages : 289
  • ISBN 10 : 0124159443

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In Situ Molecular Pathology and Co Expression Analyses Excerpt :

This new laboratory reference manual will explain, in easy-to-understand language, simplified ways to understand and perform in situ hybridization and immunohistochemistry tests. The book will also focus much attention on straightforward protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses. The fields of in situ hybridization and immunohistochemistry have expanded rapidly where computer-based analyses systems have greatly expanded the power of these methods. The automated platforms require that researchers and diagnostic biomedical investigators have a good understanding of the basics of in situ based tests, protocols and regimens for troubleshooting to maximize the use of these platforms. Practicing molecular pathologists, clinical chemists, and toxicologists, as well as clinicians and researchers in training, will benefit from this clear presentation of protocols and theoretical framework. Data derived from in situ hybridization and immunohistochemistry, especially with computer based co-expression analyses, offer a large advantage to researchers competing for federal moneys or private grants. Includes over 200 easy-to-follow experimental protocolsFeatures chapter-ending summaries of "Key Points to Remember" to bring beginners up to speed with any seasoned veteran in the fieldOffers two chapters written by industry leaders in the fields of in situ hybridization, immunohistochemistry, and computer software for co-expression analyses --

In Situ Molecular Pathology and Co expression Analyses Book

In Situ Molecular Pathology and Co expression Analyses


  • Author : Gerard J. Nuovo
  • Publisher : Academic Press
  • Release Date : 2020-09-03
  • Genre: Science
  • Pages : 418
  • ISBN 10 : 9780128206546

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In Situ Molecular Pathology and Co expression Analyses Excerpt :

Major advancements in the field of in situ molecular pathology have occurred since publication of the first edition. In Situ Molecular Pathology and Co-expression Analyses, Second Edition, continues to teach both the molecular basis for the improvements and the actual protocols. This is the unique feature that separates it from the pack of other "cook-book" type approaches. The fields of in situ hybridization and immunohistochemistry have expanded rapidly where computer-based analyses systems have greatly expanded the power of these methods. Further, knowledge of the marked improvements in the reagents themselves since the first edition can make the difference of excellent versus misleading data. The automated platforms require that researchers and diagnostic biomedical investigators have a good understanding of the basics of in situ based tests, protocols, and biochemistry for troubleshooting in order to maximize the use of these platforms. This second edition focuses attention on straightforward protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses. Practicing molecular pathologists, diagnostic pathologists, laboratory directors, and toxicologists, as well as clinicians and researchers in training, will benefit from this clear presentation of protocols and theoretical framework. Data derived from in situ hybridization and immunohistochemistry. Explains the theory and foundation of immunohistochemistry and in situ hybridization and presents easy-to-follow experimental protocols with tricks of the trade Includes two new chapters: Recent improvements in immunohistochemistry and in situ hybridization, Quality control for immunohistochemistry and in situ hybridization: How to know if the color change is signal or background The second edition also includes a detailed test to help one learn the basics of histologic interpretation of tissues and a separate detailed test in how to differentiate sign

Molecular Biology in Cellular Pathology Book

Molecular Biology in Cellular Pathology


  • Author : John Crocker
  • Publisher : John Wiley & Sons
  • Release Date : 2003-09-12
  • Genre: Science
  • Pages : 400
  • ISBN 10 : 9780470864982

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Molecular Biology in Cellular Pathology Excerpt :

The latest edition of this highly successful text, covers the major advances in the methods used in cellular and molecular pathology. In recent years, knowledge of the molecular organization of the cell has led to the development of powerful new techniques that bring greater accuracy and objectives to the diagnosis, prognosis and management of many diseases and to the study of pathological states. This book describes the latest molecular techniques available for the analysis of diseases. In particular it includes new techniques using fluorescent dyes, DNA microarrays, protein chemistry, and mass spectrometry. It also incorporates information from the Human Genome Project, and the new disciplines of genomics and proteomics, where relevant to pathology. Color plates are a new feature of this edition, illustrating the advances in fluorescence labeling of cells.

Molecular Diagnostics Book

Molecular Diagnostics


  • Author : William B. Coleman
  • Publisher : Springer Science & Business Media
  • Release Date : 2013-04-18
  • Genre: Medical
  • Pages : 390
  • ISBN 10 : 9781475725889

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Molecular Diagnostics Excerpt :

Notable practitioners describe how laboratory medicine is practiced today and illuminate how it will function tomorrow as the revolutionary advances afforded by molecular diagnostics become increasingly central to effective analysis. Proceeding from a discussion of elementary nucleic acid technology to a review of the more advanced techniques, the distinguished contributors lay the groundwork for a comprehensive understanding of their applications throughout clinical medicine. The result is a detailed description of those molecular technologies currently used in diagnostic laboratories, as well as those that seem particularly promising. Detailed discussions of specific clinical applications include those for cancer, hematological malignancies, cardiovascular disease, and neuromuscular, endocrine, and infectious diseases.

ADAMTS Proteases Book

ADAMTS Proteases


  • Author : Suneel S. Apte
  • Publisher : Humana
  • Release Date : 2020-08-29
  • Genre: Science
  • Pages : 299
  • ISBN 10 : 1493997009

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ADAMTS Proteases Excerpt :

This volume provides a variety of methods used to analyze ADAMTS proteases and ADAMTS-like proteins, including their structure, substrate profile, tissue and cell distribution, post-translational modification and biological pathways. Chapters discuss genetic, cell biology, biochemical and proteomics techniques applicable to the analysis of all the members of the ADAMTS superfamily. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, ADAMTS Proteases: Methods and Protocols is a valuable tool for researchers to study both simple and sophisticated methodologies that are often difficult to locate in primary research literature.

Handbook of Immunohistochemistry and in situ Hybridization of Human Carcinomas Book

Handbook of Immunohistochemistry and in situ Hybridization of Human Carcinomas


  • Author : M. A. Hayat
  • Publisher : Elsevier
  • Release Date : 2006-05-23
  • Genre: Medical
  • Pages : 608
  • ISBN 10 : 0080457878

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Handbook of Immunohistochemistry and in situ Hybridization of Human Carcinomas Excerpt :

Classical histology has been augmented by immunohistochemistry (the use of specific antibodies to stain particular molecular species in situ). Immunohistochemistry has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system. This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method. * The only book available that translates molecular genetics into cancer diagnosis * Methods were developed by internationally-recognized experts and presented in step-by-step manner * Results of each Immunohistochemical and in situ hybridization are presented in the form of color illustrations

Molecular Biology of the Cell Book
Score: 4.5
From 5 Ratings

Molecular Biology of the Cell


  • Author : Bruce Alberts
  • Publisher : Unknown
  • Release Date : 2004
  • Genre: Uncategoriezed
  • Pages : null
  • ISBN 10 : 0815332181

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Molecular Biology of the Cell Excerpt :

Modern Surgical Pathology Book

Modern Surgical Pathology


  • Author : Noel Weidner
  • Publisher : W B Saunders Company
  • Release Date : 2003
  • Genre: Medical
  • Pages : 1138
  • ISBN 10 : UOM:39015055923604

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Modern Surgical Pathology Excerpt :

The electronic image collection consists of JPG and PDF files containing graphics from Modern surgical pathology.

Molecular Plant Pathology Book

Molecular Plant Pathology


  • Author : Sarah Jane Gurr
  • Publisher : IRL Press
  • Release Date : 1992
  • Genre: Diseases
  • Pages : 248
  • ISBN 10 : MINN:31951D00875558P

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Molecular Plant Pathology Excerpt :

Molecular plant pathology has directly benefited from advances in modern molecular techniques. These techniques have been applied both to pathogen and plant, enhancing our understanding of the organisms themselves and of the complex interactions which determine compatibility between them and their host plants. This new book and its companion volume represent the first comprehensive guide to the latest molecular techniques as well as the established approaches to the subject. Detailed protocols are included which address a wide range of investigations from plant pathogen isolation and culture, through physiology and biochemistry, to techniques for localizing genes and their products within the cells of the infected plants. Topics in Volume I include pathogens and pathogen manipulation, nucleic acid isolation and hybridization techniques, analysis of gene expression, and in situ hybridization. Researchers in plant science, molecular biology, and related areas will find the two volumes of Molecular Plant Pathology to be an invaluable experimental resource for this exciting and fast moving field, providing a wealth of easy-to-follow protocols supported by expert advice and guidance.

Pancreatic Cancer Book

Pancreatic Cancer


  • Author : Fazlul H. Sarkar
  • Publisher : Eaton Publishing Company
  • Release Date : 1998
  • Genre: Medical
  • Pages : 192
  • ISBN 10 : CHI:56383034

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Pancreatic Cancer Excerpt :

Gene Probes Book

Gene Probes


  • Author : P. Michael Conn
  • Publisher : Academic Press
  • Release Date : 2014-04-24
  • Genre: Science
  • Pages : 478
  • ISBN 10 : 9781483267852

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Gene Probes Excerpt :

Methods in Neurosciences, Volume 1: Gene Probes is a compendium of papers that deals with the developments in molecular biology, cell biology, and electrophysiology. Section I deals with gene expression using the Xenopus Oocyte system for expression and cloning of neuroreceptors and channels. One paper presents a method in studying the molecules in the brain related to neurotransmitter receptors and to the voltage channels in the brain by ""transplanting"" functional neurotransmitter receptors into the membrane of frog oocytes. Section II describes in situ and solution hybridization and the continuation of in situ hybridization with immunohistochemistry. One paper discusses the benefits of using alkaline phosphatase-Dig-dUTP-labeled oligonucleotide probes in high-resolution in situ in terms of ease of performance, safety, and fast detection rendering. Section III addresses the screening, sequencing, and cloning, the process of which includes the rapid identification of DNA clones. One paper outlines the methods and materials that are used in such identification. Section IV explains lineage analysis and Section V discusses molecular pathology, including the molecular pathology of Alzheimer's disease. This collection can prove useful for geneticists, molecular scientists, and academicians involved in neuroscience and pharmacological sciences, as well as researchers in geriatrics.

ICN Book

ICN


  • Author : ICN Biomedicals, Inc
  • Publisher : Unknown
  • Release Date : 1995
  • Genre: Biotechnology
  • Pages : 1548
  • ISBN 10 : UOM:39015026223456

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Molecular Pathology of Gastroenterological Cancer Book

Molecular Pathology of Gastroenterological Cancer


  • Author : Eiichi Tahara
  • Publisher : Springer
  • Release Date : 1997-10
  • Genre: Medical
  • Pages : 284
  • ISBN 10 : UOM:39015041532279

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Molecular Pathology of Gastroenterological Cancer Excerpt :

Twenty years have passed since I became a professor in the First Department of Pathology, Hiroshima University School of Medicine. It is my great pleas ure that Molecular Pathology o[ Gastroenterological Cancer-Application to Clinical Practice has been published by Springer-Verlag Tokyo to commemo rate the 20th anniversary of my professorship. Seeing the academic achievements of our department during these 20 years, I am confident that we could establish a department of oncology to research the pathogenesis of human cancer through systemic application of a variety of molecular techniques. We have demonstrated that the develop me nt and progression of esophageal, gastric, and colon cancer require mul tiple alterations affecting DNA mismatch repair genes, oncogenes, and tumorsuppressor genes, and that common and uncommon genetic changes exist for esophageal, gastric, and colorectal carcinomas. In addition to these genetic changes, the majority of gastrointestinal cancers express telomerase activity, with overexpression of telomerase RNA, indicating a powerful addi tional tool for early detection of gastrointestinal cancer. By transferring these basic observations to the clinic, we now are able to make accurate cancer diagnoses, thus determing the grade of malignancy and patient prognosis. We also can identify patients at high risk for developing cancer and create new therapeutic approaches. In fact, we have routinely implemented a new molecular diagnosis strategy at the Hiroshima City Medi cal Association Clinical Laboratory since August 1993.

Molecular Diagnostics Book

Molecular Diagnostics


  • Author : George P. Patrinos
  • Publisher : Academic Press
  • Release Date : 2016-10-27
  • Genre: Medical
  • Pages : 520
  • ISBN 10 : 9780128029886

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Molecular Diagnostics Excerpt :

Molecular Diagnostics, Third Edition, focuses on the technologies and applications that professionals need to work in, develop, and manage a clinical diagnostic laboratory. Each chapter contains an expert introduction to each subject that is next to technical details and many applications for molecular genetic testing that can be found in comprehensive reference lists at the end of each chapter. Contents are divided into three parts, technologies, application of those technologies, and related issues. The first part is dedicated to the battery of the most widely used molecular pathology techniques. New chapters have been added, including the various new technologies involved in next-generation sequencing (mutation detection, gene expression, etc.), mass spectrometry, and protein-specific methodologies. All revised chapters have been completely updated, to include not only technology innovations, but also novel diagnostic applications. As with previous editions, each of the chapters in this section includes a brief description of the technique followed by examples from the area of expertise from the selected contributor. The second part of the book attempts to integrate previously analyzed technologies into the different aspects of molecular diagnostics, such as identification of genetically modified organisms, stem cells, pharmacogenomics, modern forensic science, molecular microbiology, and genetic diagnosis. Part three focuses on various everyday issues in a diagnostic laboratory, from genetic counseling and related ethical and psychological issues, to safety and quality management. Presents a comprehensive account of all new technologies and applications used in clinical diagnostic laboratories Explores a wide range of molecular-based tests that are available to assess DNA variation and changes in gene expression Offers clear translational presentations by the top molecular pathologists, clinical chemists, and molecular geneticists in the field