Introduction to Protein Mass Spectrometry Book

Introduction to Protein Mass Spectrometry


  • Author : Pradip Kumar Ghosh
  • Publisher : Academic Press
  • Release Date : 2015-12-10
  • Genre: Science
  • Pages : 312
  • ISBN 10 : 9780128021125

GET BOOK
Introduction to Protein Mass Spectrometry Excerpt :

Introduction to Protein Mass Spectrometry provides a comprehensive overview of this increasingly important, yet complex, analytical technique. Unlike many other methods which automatically yield an absolutely unique protein name as output, protein mass spectrometry generally requires a deduction of protein identity from determination of peptide fragmentation products. This book enables readers to both understand, and appreciate, how determinations about protein identity from mass spectrometric data are made. Coverage begins with the technical basics, including preparations, instruments, and spectrometric analysis of peptides and proteins, before exploring applied use in biological applications, bioinformatics, database, and software resources. Citing the most recent and relevant work in the field of biological mass spectrometry, the book is written for researchers and scientists new to the field, but is also an ideal resource for those hoping to hone their analytical abilities. Offers introductory information for scientists and researchers new to the field, as well as advanced insight into the critical assessment of computer-analyzed mass spectrometric results and their current limitations Provides examples of commonly-used MS instruments from Bruker, Applied Biosystems, JEOL, Thermo Scientific/Thermo Fisher Scientific, IU, and Waters Includes biological applications and exploration of analytical tools and databases for bioinformatics

Introduction to Protein Mass Spectrometry Book

Introduction to Protein Mass Spectrometry


  • Author : Pradip Kumar Ghosh
  • Publisher : Academic Press
  • Release Date : 2015-12-15
  • Genre: Uncategoriezed
  • Pages : 350
  • ISBN 10 : 012805123X

GET BOOK
Introduction to Protein Mass Spectrometry Excerpt :

Introduction to Protein Mass Spectrometry provides a comprehensive overview of this increasingly important, yet complex, analytical technique. Unlike many other methods which automatically yield an absolutely unique protein name as output, protein mass spectrometry generally requires a deduction of protein identity from determination of peptide fragmentation products. This book enables readers to both understand, and appreciate, how determinations about protein identity from mass spectrometric data are made. Coverage begins with the technical basics, including preparations, instruments, and spectrometric analysis of peptides and proteins, before exploring applied use in biological applications, bioinformatics, database, and software resources. Citing the most recent and relevant work in the field of biological mass spectrometry, the book is written for researchers and scientists new to the field, but is also an ideal resource for those hoping to hone their analytical abilities. Offers introductory information for scientists and researchers new to the field, as well as advanced insight into the critical assessment of computer-analyzed mass spectrometric results and their current limitations Provides examples of commonly-used MS instruments from Bruker, Applied Biosystems, JEOL, Thermo Scientific/Thermo Fisher Scientific, IU, and Waters Includes biological applications and exploration of analytical tools and databases for bioinformatics

Protein Sequencing and Identification Using Tandem Mass Spectrometry Book

Protein Sequencing and Identification Using Tandem Mass Spectrometry


  • Author : Michael Kinter
  • Publisher : John Wiley & Sons
  • Release Date : 2005-04-12
  • Genre: Science
  • Pages : 320
  • ISBN 10 : 9780471231882

GET BOOK
Protein Sequencing and Identification Using Tandem Mass Spectrometry Excerpt :

How to design, execute, and interpret experiments for proteinsequencing using mass spectrometry The rapid expansion of searchable protein and DNA databases inrecent years has triggered an explosive growth in the applicationof mass spectrometry to protein sequencing. This timely andauthoritative book provides professionals and scientists inbiotechnology research with complete coverage of procedures foranalyzing protein sequences by mass spectrometry, includingstep-by-step guidelines for sample preparation, analysis, and datainterpretation. Michael Kinter and Nicholas Sherman present their ownhigh-quality, laboratory-tested protocols for the analysis of awide variety of samples, demonstrating how to carry out specificexperiments and obtain fast, reliable results with a 99% successrate. Readers will get sufficient experimental detail to apply intheir own laboratories, learn about the proper selection andoperation of instruments, and gain essential insight into thefundamental principles of mass spectrometry and protein sequencing.Coverage includes: Peptide fragmentation and interpretation of product ionspectra Basic polyacrylamide gel electrophoresis Preparation of protein digests for sequencing experiments Mass spectrometric analysis using capillary liquidchromatography Techniques for protein identification by database searches Characterization of modified peptides using tandem massspectrometry And much more

Characterization of Protein Therapeutics using Mass Spectrometry Book

Characterization of Protein Therapeutics using Mass Spectrometry


  • Author : Guodong Chen
  • Publisher : Springer Science & Business Media
  • Release Date : 2014-07-08
  • Genre: Science
  • Pages : 404
  • ISBN 10 : 9781441978622

GET BOOK
Characterization of Protein Therapeutics using Mass Spectrometry Excerpt :

This book highlights current approaches and future trends in the use of mass spectrometry to characterize protein therapies. As one of the most frequently utilized analytical techniques in pharmaceutical research and development, mass spectrometry has been widely used in the characterization of protein therapeutics due to its analytical sensitivity, selectivity, and specificity. This book begins with an overview of mass spectrometry techniques as related to the analysis of protein therapeutics, structural identification strategies, quantitative approaches, followed by studies involving characterization of process related protein drug impurities/degradants, metabolites, higher order structures of protein therapeutics. Both general practitioners in pharmaceutical research and specialists in analytical sciences will benefit from this book that details step-by-step approaches and new strategies to solve challenging problems related to protein therapeutics research and development.

Biological Mass Spectrometry Book

Biological Mass Spectrometry


  • Author : A.L. Burlingame
  • Publisher : Gulf Professional Publishing
  • Release Date : 2005-12-12
  • Genre: Science
  • Pages : 478
  • ISBN 10 : 0121828077

GET BOOK
Biological Mass Spectrometry Excerpt :

This volume describes and integrates the techniques and fundamentals of more than a decade of revolutionary advances in both chromatographic and mass spectrometric technologies that have enabled the direct investigation of biomacromolecules per se and have provided the analytical power base to usher in the new fields of proteomics and systems biology. It also covers new biophysical applications such as H/D exchange for study of conformations, protein-protein and protein-metal and ligand interactions. Finally it describes atto-to-zepto-mole quantitation of 14C and 3H by accelerator mass spectrometry. *Part 1 of 2 volumes about Mass Spectrometry *Authoritative and comprehensive treatment of protein mass spectrometry in human cell biology *Presents fundamentals, techniques, instrumentation and bioinformatics *Provides an overview of proteomics, protein-protein and protein-ligand binding, and biophysical studies

Plant Systems Biology Book

Plant Systems Biology


  • Author : Sacha Baginsky
  • Publisher : Springer Science & Business Media
  • Release Date : 2007-02-16
  • Genre: Science
  • Pages : 358
  • ISBN 10 : 9783764372613

GET BOOK
Plant Systems Biology Excerpt :

This volume aims to provide a timely view of the state-of-the-art in systems biology. The editors take the opportunity to define systems biology as they and the contributing authors see it, and this will lay the groundwork for future studies. The volume is well-suited to both students and researchers interested in the methods of systems biology. Although the focus is on plant systems biology, the proposed material could be suitably applied to any organism.

Neuroproteomics Book

Neuroproteomics


  • Author : Oscar Alzate
  • Publisher : CRC Press
  • Release Date : 2009-10-26
  • Genre: Science
  • Pages : 354
  • ISBN 10 : 1420076264

GET BOOK
Neuroproteomics Excerpt :

In this, the post-genomic age, our knowledge of biological systems continues to expand and progress. As the research becomes more focused, so too does the data. Genomic research progresses to proteomics and brings us to a deeper understanding of the behavior and function of protein clusters. And now proteomics gives way to neuroproteomics as we begin to unravel the complex mysteries of neurological diseases that less than a generation ago seemed opaque to our inquiries, if not altogether intractable. Edited by Dr. Oscar Alzate, Neuroproteomics is the newest volume in the CRC Press Frontiers of Neuroscience Series. With an extensive background in mathematics and physics, Dr. Alzate exemplifies the newest generation of biological systems researchers. He organizes research and data contributed from all across the world to present an overview of neuroproteomics that is practical and progressive. Bolstered by each new discovery, researchers employing multiple methods of inquiry gain a deeper understanding of the key biological problems related to brain function, brain structure, and the complexity of the nervous system. This in turn is leading to new understanding about diseases of neurological deficit such as Parkinson’s and Alzheimer’s. Approaches discussed in the book include mass spectrometry, electrophoresis, chromatography, surface plasmon resonance, protein arrays, immunoblotting, computational proteomics, and molecular imaging. Writing about their own work, leading researchers detail the principles, approaches, and difficulties of the various techniques, demonstrating the questions that neuroproteomics can answer and those it raises. New challenges wait, not the least of which is the identification of potential methods to regulate the structures and functions of key protein interaction networks. Ultimately, those building on the foundation presented here will advance our understanding of the brain and show us ways to abate the suffering caused by neurological

Computational and Statistical Methods for Protein Quantification by Mass Spectrometry Book

Computational and Statistical Methods for Protein Quantification by Mass Spectrometry


  • Author : Ingvar Eidhammer
  • Publisher : John Wiley & Sons
  • Release Date : 2012-12-10
  • Genre: Mathematics
  • Pages : 360
  • ISBN 10 : 9781118493779

GET BOOK
Computational and Statistical Methods for Protein Quantification by Mass Spectrometry Excerpt :

The definitive introduction to data analysis in quantitativeproteomics This book provides all the necessary knowledge about massspectrometry based proteomics methods and computational andstatistical approaches to pursue the planning, design and analysisof quantitative proteomics experiments. The author’scarefully constructed approach allows readers to easily make thetransition into the field of quantitative proteomics. Throughdetailed descriptions of wet-lab methods, computational approachesand statistical tools, this book covers the full scope of aquantitative experiment, allowing readers to acquire new knowledgeas well as acting as a useful reference work for more advancedreaders. Computational and Statistical Methods for ProteinQuantification by Mass Spectrometry: Introduces the use of mass spectrometry in proteinquantification and how the bioinformatics challenges in this fieldcan be solved using statistical methods and various softwareprograms. Is illustrated by a large number of figures and examples aswell as numerous exercises. Provides both clear and rigorous descriptions of methods andapproaches. Is thoroughly indexed and cross-referenced, combining thestrengths of a text book with the utility of a reference work. Features detailed discussions of both wet-lab approaches andstatistical and computational methods. With clear and thorough descriptions of the various methods andapproaches, this book is accessible to biologists, informaticians,and statisticians alike and is aimed at readers across the academicspectrum, from advanced undergraduate students to post doctoratesentering the field.

Analyzing Biomolecular Interactions by Mass Spectrometry Book

Analyzing Biomolecular Interactions by Mass Spectrometry


  • Author : Jeroen Kool
  • Publisher : John Wiley & Sons
  • Release Date : 2015-05-04
  • Genre: Science
  • Pages : 400
  • ISBN 10 : 9783527334643

GET BOOK
Analyzing Biomolecular Interactions by Mass Spectrometry Excerpt :

This monograph reviews all relevant technologies based on mass spectrometry that are used to study or screen biological interactions in general. Arranged in three parts, the text begins by reviewing techniques nowadays almost considered classical, such as affinity chromatography and ultrafiltration, as well as the latest techniques. The second part focusses on all MS-based methods for the study of interactions of proteins with all classes of biomolecules. Besides pull down-based approaches, this section also emphasizes the use of ion mobility MS, capture-compound approaches, chemical proteomics and interactomics. The third and final part discusses other important technologies frequently employed in interaction studies, such as biosensors and microarrays. For pharmaceutical, analytical, protein, environmental and biochemists, as well as those working in pharmaceutical and analytical laboratories.

Hydrogen Exchange Mass Spectrometry of Proteins Book

Hydrogen Exchange Mass Spectrometry of Proteins


  • Author : David D. Weis
  • Publisher : John Wiley & Sons
  • Release Date : 2016-03-21
  • Genre: SCIENCE
  • Pages : 376
  • ISBN 10 : 9781118616499

GET BOOK
Hydrogen Exchange Mass Spectrometry of Proteins Excerpt :

Hydrogen exchange mass spectrometry is widely recognized for its ability to probe the structure and dynamics of proteins. The application of this technique is becoming widespread due to its versatility for providing structural information about challenging biological macromolecules such as antibodies, flexible proteins and glycoproteins. Although the technique has been around for 25 years, this is the first definitive book devoted entirely to the topic. Hydrogen Exchange Mass Spectrometry of Proteins: Fundamentals, Methods and Applications brings into one comprehensive volume the theory, instrumentation and applications of Hydrogen Exchange Mass Spectrometry (HX-MS) - a technique relevant to bioanalytical chemistry, protein science and pharmaceuticals. The book provides a solid foundation in the basics of the technique and data interpretation to inform readers of current research in the method, and provides illustrative examples of its use in bio- and pharmaceutical chemistry and biophysics In-depth chapters on the fundamental theory of hydrogen exchange, and tutorial chapters on measurement and data analysis provide the essential background for those ready to adopt HX-MS. Expert users may advance their current understanding through chapters on methods including membrane protein analysis, alternative proteases, millisecond hydrogen exchange, top-down mass spectrometry, histidine exchange and method validation. All readers can explore the diversity of HX-MS applications in areas such as ligand binding, membrane proteins, drug discovery, therapeutic protein formulation, biocomparability, and intrinsically disordered proteins.

Mass Spectrometry Data Analysis in Proteomics Book

Mass Spectrometry Data Analysis in Proteomics


  • Author : Rune Matthiesen
  • Publisher : Unknown
  • Release Date : 2013
  • Genre: Mass spectrometry
  • Pages : 405
  • ISBN 10 : 1627033920

GET BOOK
Mass Spectrometry Data Analysis in Proteomics Excerpt :

Since the publishing of the first edition, the methodologies and instrumentation involved in the field of mass spectrometry-based proteomics has improved considerably. Fully revised and expanded, Mass Spectrometry Data Analysis in Proteomics, Second Edition presents expert chapters on specific MS-based methods or data analysis strategies in proteomics. The volume covers data analysis topics relevant for quantitative proteomics, post translational modification, HX-MS, glycomics, and data exchange standards, among other topics. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Updated and authoritative, Mass Spectrometry Data Analysis in Proteomics, Second Edition serves as a detailed guide for all researchers seeking to further our knowledge in the field of proteomics.

Introduction to Proteomics Book
Score: 1
From 1 Ratings

Introduction to Proteomics


  • Author : Daniel Liebler
  • Publisher : Springer Science & Business Media
  • Release Date : 2001-12-04
  • Genre: Medical
  • Pages : 198
  • ISBN 10 : 9780896039919

GET BOOK
Introduction to Proteomics Excerpt :

Daniel C. Liebler masterfully introduces the science of proteomics by spelling out the basics of how one analyzes proteins and proteomes, and just how these approaches are then employed to investigate their roles in living systems. He explains the key concepts of proteomics, how the analytical instrumentation works, what data mining and other software tools do, and how these tools can be integrated to study proteomes. Also discussed are how protein and peptide separation techniques are applied in proteomics, how mass spectrometry is used to identify proteins, and how data analysis software enables protein identification and the mapping of modifications. In addition, there are proteomic approaches for analyzing differential protein expression, characterizing proteomic diversity, and dissecting protein-protein interactions and networks.

Introduction to Peptides and Proteins Book

Introduction to Peptides and Proteins


  • Author : Ulo Langel
  • Publisher : CRC Press
  • Release Date : 2009-11-18
  • Genre: Medical
  • Pages : 420
  • ISBN 10 : 9781439882047

GET BOOK
Introduction to Peptides and Proteins Excerpt :

Human cells produce at least 30,000 different proteins. Each has a specific function characterized by a unique sequence and native conformation that allows it to perform that function. While research in this post-genomic era has created a deluge of invaluable information, the field has lacked for an authoritative introductory text needed to inform researchers and students in all of those fields now concerned with protein research. Introduction to Peptides and Proteins brings together some of the most respected researchers in protein science to present a remarkably coherent introduction to modern peptide and protein chemistry. The first sections of the book delve into – Basic peptide and protein science from assembly through degradation Traditional and emerging research methods including those used in bioinformatics and proteomics New computational approaches and algorithms used to find patterns in the vast data collected by sequencing projects After providing a foundation in tools and methods, the authors closely examine six protein families, including representative classes such as enzymes, cell-surface receptors, antibodies, fibrous proteins, and bioactive peptide classes. They concentrate on biochemical mechanisms and where possible indicate therapeutic or biotechnical possibilities. Then focusing on clinical aspects, the authors investigate misfolding as found in prion diseases, miscleavage as found in Alzheimer’s, and mis-sequencing as found with some cancers. Drawing from some of their own research, the authors summarize recent achievements and emerging applications. They discuss the use of proteins and peptides as drugs and the solid-phase synthesis required for drug production. They also look at the use of peptides as functional biomolecules and research tools. No longer just the domain of biologists, many key advances in protein research started in physics labs and have involved contributions from a host of fields including statistics, drug development,

Proteomics and Protein Protein Interactions Book
Score: 4
From 1 Ratings

Proteomics and Protein Protein Interactions


  • Author : Gabriel Waksman
  • Publisher : Springer Science & Business Media
  • Release Date : 2005-12-21
  • Genre: Medical
  • Pages : 323
  • ISBN 10 : 0387245316

GET BOOK
Proteomics and Protein Protein Interactions Excerpt :

The rapidly evolving field of protein science has now come to realize the ubiquity and importance of protein-protein interactions. It had been known for some time that proteins may interact with each other to form functional complexes, but it was thought to be the property of only a handful of key proteins. However, with the advent of high throughput proteomics to monitor protein-protein interactions at an organism level, we can now safely state that protein-protein interactions are the norm and not the exception. Thus, protein function must be understood in the larger context of the various binding complexes that each protein may form with interacting partners at a given time in the life cycle of a cell. Proteins are now seen as forming sophisticated interaction networks subject to remarkable regulation. The study of these interaction networks and regulatory mechanism, which I would like to term "systems proteomics," is one of the thriving fields of proteomics. The bird-eye view that systems proteomics offers should not however mask the fact that proteins are each characterized by a unique set of physical and chemical properties. In other words, no protein looks and behaves like another. This complicates enormously the design of high-throughput proteomics methods. Unlike genes, which, by and large, display similar physico-chemical behaviors and thus can be easily used in a high throughput mode, proteins are not easily amenable to the same treatment. It is thus important to remind researchers active in the proteomics field the fundamental basis of protein chemistry. This book attempts to bridge the two extreme ends of protein science: on one end, systems proteomics, which describes, at a system level, the intricate connection network that proteins form in a cell, and on the other end, protein chemistry and biophysics, which describe the molecular properties of individual proteins and the structural and thermodynamic basis of their interactions within the network. Br

Proteomic Profiling and Analytical Chemistry Book

Proteomic Profiling and Analytical Chemistry


  • Author : Pawel Ciborowski
  • Publisher : Elsevier
  • Release Date : 2016-03-02
  • Genre: Science
  • Pages : 298
  • ISBN 10 : 9780444636904

GET BOOK
Proteomic Profiling and Analytical Chemistry Excerpt :

Proteomic Profiling and Analytical Chemistry: The Crossroads, Second Edition helps scientists without a strong background in analytical chemistry to understand principles of the multistep proteomic experiment necessary for its successful completion. It also helps researchers who do have an analytical chemistry background to break into the proteomics field. Highlighting points of junction between proteomics and analytical chemistry, this resource links experimental design with analytical measurements, data analysis, and quality control. This targeted point of view will help both biologists and chemists to better understand all components of a complex proteomic study. The book provides detailed coverage of experimental aspects such as sample preparation, protein extraction and precipitation, gel electrophoresis, microarrays, dynamics of fluorescent dyes, and more. The key feature of this book is a direct link between multistep proteomic strategy and quality control routinely applied in analytical chemistry. This second edition features a new chapter on SWATH-MS, substantial updates to all chapters, including proteomic database search and analytical quantification, expanded discussion of post-hoc statistical tests, and additional content on validation in proteomics. Covers the analytical consequences of protein and peptide modifications that may have a profound effect on how and what researchers actually measure Includes practical examples illustrating the importance of problems in quantitation and validation of biomarkers Helps in designing and executing proteomic experiments with sound analytics