Microfluidic Cell Culture Systems Book

Microfluidic Cell Culture Systems


  • Author : Christopher Bettinger
  • Publisher : William Andrew
  • Release Date : 2012-12-31
  • Genre: Science
  • Pages : 452
  • ISBN 10 : 9781437734607

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Microfluidic Cell Culture Systems Book Description :

The fields of microfluidics and BioMEMS are significantly impacting cell biology research and applications through the application of engineering solutions to human disease and health problems. The dimensions of microfluidic channels are well suited to the physical scale of biological cells, and the many advantages of microfluidics make it an attractive platform for new techniques in biology. This new professional reference applies the techniques of microsystems to cell culture applications. The authors provide a thoroughly practical guide to the principles of microfluidic device design and operation and their application to cell culture techniques. The resulting book is crammed with strategies and techniques that can be immediately deployed in the lab. Equally, the insights into cell culture applications will provide those involved in traditional microfluidics and BioMEMS with an understanding of the specific demands and opportunities presented by biological applications. The goal is to guide new and interested researchers and technology developers to the important areas and state-of-the-practice strategies that will enhance the efficiency and value of their technologies, devices and biomedical products. Provides insights into the design and development of microfluidic systems with a specific focus on cell culture applications Focuses on strategies and techniques for the design and fabrication of microfluidic systems and devices for cell culture Provides balanced coverage of microsystems engineering and bioengineering

Microfluidic Cell Culture Systems Book

Microfluidic Cell Culture Systems


  • Author : Christopher Bettinger
  • Publisher : William Andrew Pub
  • Release Date : 2013
  • Genre: Science
  • Pages : 433
  • ISBN 10 : 1437734596

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Microfluidic Cell Culture Systems Book Description :

The fields of microfluidics and BioMEMS are signigicantly impacting cell biology research and applications through the application of engineering solutions to human disease and health problems. The dimensions of microfluidic channels are well suited to the physical scale of biological cells, and the many advantages of microfluidics make it an attractive platform for new techniques in biology. One of the key benefits of microfluidics for bioengineering is the ability to control parameters of the cell microenvironment at relevant length and time scales. This new professional reference applies the techniques of microsystems to cell culture applications. The authors provide a thoroughly practical guide to the principles of microfluidic device design and operation, and their application to cell culture techniques. The resulting book is crammed with strategies and techniques that can be immediately deployed in the lab. Equally, the insights into cell culture applications will provide those involved in traditional microfluidics and bioMEMS with an understanding of the specific demands and opportunities presented by biological applications. The goal is to guide new and interested researchers and technology developers to the important areas and state-of-the-practice strategies that will enhance the efficiency and value of their technolgies, devices and biomedical products. Provides insights into the design and development of microfluidic systems with a specific focus on cell culture applications. Focuses on strategies and techniques for the design and fabrication of microfluidic systems and devices for cell culture. Provides a balanced coverage of microsystems engineering and bioengineering.

Cardiac Cell Culture Technologies Book

Cardiac Cell Culture Technologies


  • Author : Zbigniew Brzozka
  • Publisher : Springer
  • Release Date : 2017-11-21
  • Genre: Technology & Engineering
  • Pages : 234
  • ISBN 10 : 9783319706856

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Cardiac Cell Culture Technologies Book Description :

This book provides an introduction to the biological background of heart functioning and analyzes the various materials and technologies used for the development of microfluidic systems dedicated to cell culture, with an emphasis on cardiac cells. The authors describe the characterization of microfluidic systems for cardiac cell culture and center their discussion of the use of stem cell stimulation based on four different types: electrical, biochemical, physical, and mechanical. This book is appropriate for researchers focused on on-chip technologies and heart studies, students in bioengineering and microengineering courses, and a variety of professionals, such as biotechnologists, biomedical engineers, and clinicians working in the cardiac diseases field.

Microfluidics for Single Cell Analysis Book

Microfluidics for Single Cell Analysis


  • Author : Jin-Ming Lin
  • Publisher : Springer Nature
  • Release Date : 2019-08-28
  • Genre: Science
  • Pages : 261
  • ISBN 10 : 9789813297296

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Microfluidics for Single Cell Analysis Book Description :

This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor’s laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.

Microfluidic Cell Culture Systems Book

Microfluidic Cell Culture Systems


  • Author : Jeffrey T Borenstein
  • Publisher : Elsevier
  • Release Date : 2018-09-12
  • Genre: Science
  • Pages : 396
  • ISBN 10 : 9780128136720

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Microfluidic Cell Culture Systems Book Description :

Techniques for microfabricating intricate microfluidic structures that mimic the microenvironment of tissues and organs, combined with the development of biomaterials with carefully engineered surface properties, have enabled new paradigms in and cell culture-based models for human diseases. The dimensions of surface features and fluidic channels made accessible by these techniques are well-suited to the size scale of biological cells. Microfluidic Cell Culture Systems applies design and experimental techniques used in in microfluidics, and cell culture technologies to organ-on-chip systems. This book is intended to serve as a professional reference, providing a practical guide to design and fabrication of microfluidic systems and biomaterials for use in cell culture systems and human organ models. The book covers topics ranging from academic first principles of microfluidic design, to clinical translation strategies for cell culture protocols. The goal is to help professionals coming from an engineering background to adapt their expertise for use in cell culture and organ models applications, and likewise to help biologists to design and employ microfluidic technologies in their cell culture systems. This 2nd edition contains new material that strengthens the focus on in vitro models useful for drug discovery and development. One new chapter reviews liver organ models from an industry perspective, while others cover new technologies for scaling these models and for multi-organ systems. Other new chapters highlight the development of organ models and systems for specific applications in disease modeling and drug safety. Previous chapters have been revised to reflect the latest advances. Provides design and operation methodology for microfluidic and microfabricated materials and devices for organ-on-chip disease and safety models. This is a rapidly expanding field that will continue to grow along with advances in cell biology and microfluidics technologies. Comprehen

Medical and Industrial Applications of Microfluidic based Cell Tissue Culture and Organs on a Chip  Advances in Organs on a Chip and Organoids Technologies Book

Medical and Industrial Applications of Microfluidic based Cell Tissue Culture and Organs on a Chip Advances in Organs on a Chip and Organoids Technologies


  • Author : Qasem Ramadan
  • Publisher : Frontiers Media SA
  • Release Date : 2019-10-16
  • Genre:
  • Pages :
  • ISBN 10 : 9782889631148

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Medical and Industrial Applications of Microfluidic based Cell Tissue Culture and Organs on a Chip Advances in Organs on a Chip and Organoids Technologies Book Description :

Recent developments in microfluidics have demonstrated enormous potential of microscale cell culture for biology studies and recognized as instrumental in performing rapid and efficient experiments on small-sample volumes. Microfluidic-based cell culture is an area of research that keeps growing and gaining importance as a prominent technology, able to link scientific disciplines with industrial and clinical applications. In particular, organotypic cell culture and its integration in microfluidic devices would enable the realization of “in vivo-like” cell microenvironment within systems that are more amenable to automation and integration. Such remarkable advancement forms the foundation and motivation to transfer research from the laboratory to the field. Although the microfluidics and cell culture technologies have influenced many areas of science, significant research efforts are currently focus on finding methods to transform drug screening and toxicity testing from a system reliant on high-dose animal studies to one based primarily on human-relevant in vitro models. In line with regulatory developments precluding the use of animal testing, as well as fundamental differences in animal versus human, human in vitro methodologies are required to replace the animal-based testes while permitting physiologically relevant model equivalents for superior prediction. Organs-on-a-chip is an ambitious and rapidly growing technology that promise to bridge the gap between in vivo and in vitro studies and open wide possibilities in medical and industrial applications. However, many challenges are still ahead. This eBook present recent state-of-the-art works and critical reviews in organs-on-a-chip technology which highlight the new advances in this growing field with an emphasis on the interface between technological advancements and high impact applications.

Advances in Microfluidics Book

Advances in Microfluidics


  • Author : Xiao-Ying Yu
  • Publisher : BoD – Books on Demand
  • Release Date : 2016-11-23
  • Genre: Science
  • Pages : 422
  • ISBN 10 : 9789535127857

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Advances in Microfluidics Book Description :

Increasing innovations and applications make microfluidics a versatile choice for researchers in many disciplines. This book consists of multiple review chapters that aim to cover recent advances and new applications of microfluidics in biology, electronics, energy, and materials sciences. It provides comprehensive views of various aspects of microfluidics ranging from fundamentals of fabrication, flow control, and droplet manipulation to the most recent exploration in emerging areas such as material synthesis, imaging and novel spectroscopy, and marriage with electronics. The chapters have many illustrations showcasing exciting results. This book should be useful for those who are eager to learn more about microfluidics as well as researchers who want to pick up new concepts and developments in this fast-growing field.

Cell Analysis on Microfluidics Book

Cell Analysis on Microfluidics


  • Author : Jin-Ming Lin
  • Publisher : Springer
  • Release Date : 2017-10-25
  • Genre: Science
  • Pages : 429
  • ISBN 10 : 9789811053948

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Cell Analysis on Microfluidics Book Description :

This book presents a detailed overview of the design, formatting, application, and development of microfluidic chips in the context of cell biology research, enumerating each element involved in microfluidics-based cell analysis, discussing its history, status quo, and future prospects, It also offers an extensive review of the research completed in the past decade, including numerous color figures. The individual chapters are based on the respective authors' studies and experiences, providing tips from the frontline to help researchers overcome bottlenecks in their own work. It highlights a number of cutting-edge techniques, such as 3D cell culture, microfluidic droplet technique, and microfluidic chip-mass spectrometry interfaces, offering a first-hand impression of the latest trends in the field and suggesting new research directions. Serving as both an elementary introduction and advanced guidebook, the book interests and inspires scholars and students who are currently studying microfluidics-based cell analysis methods as well as those who wish to do so.

Organ on a chip Book

Organ on a chip


  • Author : Julia Hoeng
  • Publisher : Academic Press
  • Release Date : 2019-11-09
  • Genre: Technology & Engineering
  • Pages : 546
  • ISBN 10 : 9780128172032

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Organ on a chip Book Description :

Organ-on-a-Chip: Engineered Microenvironments for Safety and Efficacy Testing contains chapters from world-leading researchers in the field of organ on a chip development and applications, with perspectives from life sciences, medicine, physiology and engineering. The book contains an overview of the field, with sections covering the major organ systems and currently available technologies, platforms and methods. As readers may also be interested in creating biochips, materials and engineering best practice, these topics are also described. Users will learn about the limitations of 2D in-vitro models and the available 3D in-vitro models (what benefits they offer and some examples). Finally, the MOC section shows how the organ on a chip technology can be adapted to improve the physiology of in-vitro models. Includes case studies of other organs on a chip that have been developed and successfully used Provides insights into functional microphysiological organ on a chip platforms for toxicity and efficacy testing, along with opportunities for translational medicine Presented fields (PK/PD, physiology, medicine, safety) are given a definition followed by the challenges and potential of organs on a chip

Microfluidics in Cell BiologyPart A  Microfluidics for multicellular systems Book

Microfluidics in Cell BiologyPart A Microfluidics for multicellular systems


  • Author :
  • Publisher : Academic Press
  • Release Date : 2018-07-20
  • Genre: Science
  • Pages : 273
  • ISBN 10 : 9780128142813

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Microfluidics in Cell BiologyPart A Microfluidics for multicellular systems Book Description :

Microfluidics in Cell Biology Part A: Volume 146, the latest release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are sections on Cell monolayers/spheroids, Collective migration in microtubes, Leukocyte adhesion dynamics on endothelial monolayers under flow, Constrained spheroid for perfusion culture, Cells in droplet arrays, Heart on chips, Kidney on chips, Liver on chips and hepatic immune responses, Gut on chips, 3D microvascular model-based lymphoma model, Blood brain barrier on chips, Multi-organ-on-a-chip for pharmacokinetic analysis, Cancer immunotherapy on chips, and more. Contains contributions from experts in the field from across the globe Covers a wide array of topics on both mitosis and meiosis Includes relevant, analysis based topics

Microfluidics in Cell Biology Part C  Microfluidics for Cellular and Subcellular Analysis Book

Microfluidics in Cell Biology Part C Microfluidics for Cellular and Subcellular Analysis


  • Author :
  • Publisher : Academic Press
  • Release Date : 2018-11-22
  • Genre: Science
  • Pages : 264
  • ISBN 10 : 9780128142851

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Microfluidics in Cell Biology Part C Microfluidics for Cellular and Subcellular Analysis Book Description :

Microfluidics in Cell Biology Part C, Volume 148, a new release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are three sections on microfluidics in various multi-cellular models, including microfluidics in cell monolayers/spheroids, microfluidics in organ on chips, and microfluidics in model organisms. Specific chapters discuss collective migration in microtubes, leukocyte adhesion dynamics on endothelial monolayers under flow, constrained spheroid for perfusion culture, cells in droplet arrays, heart on chips, kidney on chips, liver on chips, and more. Contains contributions from experts in the field from across the world Covers a wide array of topics on both mitosis and meiosis Includes relevant, analysis based topics

Lab on a Chip Fabrication and Application Book

Lab on a Chip Fabrication and Application


  • Author : Margarita Stoytcheva
  • Publisher : BoD – Books on Demand
  • Release Date : 2016-06-29
  • Genre: Science
  • Pages : 208
  • ISBN 10 : 9789535124573

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Lab on a Chip Fabrication and Application Book Description :

The necessity of on-site, fast, sensitive, and cheap complex laboratory analysis, associated with the advances in the microfabrication technologies and the microfluidics, made it possible for the creation of the innovative device lab-on-a-chip (LOC), by which we would be able to scale a single or multiple laboratory processes down to a chip format. The present book is dedicated to the LOC devices from two points of view: LOC fabrication and LOC application.

Animal Biotechnology Book

Animal Biotechnology


  • Author : Ashish S. Verma
  • Publisher : Academic Press
  • Release Date : 2020-07-03
  • Genre: Medical
  • Pages : 798
  • ISBN 10 : 9780128117101

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Animal Biotechnology Book Description :

Animal Biotechnology: Models in Discovery and Translation, Second Edition, provides a helpful guide to anyone seeking a thorough review of animal biotechnology and its application to human disease and welfare. This updated edition covers vital fundamentals, including animal cell cultures, genome sequencing analysis, epigenetics and animal models, gene expression, and ethics and safety concerns, along with in-depth examples of implications for human health and prospects for the future. New chapters cover animal biotechnology as applied to various disease types and research areas, including in vitro fertilization, human embryonic stem cell research, biosensors, enteric diseases, biopharming, organ transplantation, tuberculosis, neurodegenerative disorders, and more. Highlights the latest biomedical applications of genetically modified and cloned animals, with a focus on cancer and infectious diseases Offers first-hand accounts of the use of biotechnology tools, including molecular markers, stem cells, animal cultures, tissue engineering, ADME and CAM Assay Includes case studies that illustrate safety assessment issues, ethical considerations, and intellectual property rights associated with the translation of animal biotechnology studies

Applications of Microfluidic Systems in Biology and Medicine Book

Applications of Microfluidic Systems in Biology and Medicine


  • Author : Manabu Tokeshi
  • Publisher : Springer
  • Release Date : 2019-04-25
  • Genre: Science
  • Pages : 382
  • ISBN 10 : 9789811362293

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Applications of Microfluidic Systems in Biology and Medicine Book Description :

This book focuses on state-of-the-art microfluidic research in medical and biological applications. The top-level researchers in this research field explain carefully and clearly what can be done by using microfluidic devices. Beginners in the field —undergraduates, engineers, biologists, medical researchers—will easily learn to understand microfluidic-based medical and biological applications. Because a wide range of topics is summarized here, it also helps experts to learn more about fields outside their own specialties. The book covers many interesting subjects, including cell separation, protein crystallization, single-cell analysis, cell diagnosis, point-of-care testing, immunoassay, embyos/worms on a chip and organ-on-a-chip. Readers will be convinced that microfluidic devices have great potential for medical and biological applications.

Culturing Life Book

Culturing Life


  • Author : Hannah Landecker
  • Publisher : Harvard University Press
  • Release Date : 2009-06-30
  • Genre: Science
  • Pages : 288
  • ISBN 10 : 9780674039902

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Culturing Life Book Description :

How did cells make the journey from their origin in living bodies to something that can be grown and manipulated on artificial media in the laboratory? This is the question at the heart of Hannah Landecker's book. She shows how cell culture changed the way we think about such central questions of the human condition as individuality, hybridity, and even immortality and asks what it means that we can remove cells from the spatial constraints of the body and "harness them to human intention."

3D Cell Culture Book

3D Cell Culture


  • Author : Zuzana Koledova
  • Publisher : Humana Press
  • Release Date : 2017-06-21
  • Genre: Science
  • Pages : 452
  • ISBN 10 : 1493970194

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3D Cell Culture Book Description :

This book provides an overview of established 3D cell culture assays from leaders in the field. Their contributions cover a wide spectrum of techniques and approaches for 3D cell culture, from organoid cultures through organotypic models to microfluidic approaches and emerging 3D bioprinting techniques, which are used in developmental, stem cell, cancer, and pharmacological studies, among many others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, 3D Cell Culture: Methods and Protocols aims to inspire researchers to develop novel 3D cell culture techniques according to their specific scientific needs and interests, leading to a new generation of physiologically relevant and realistic 3D cell cultures. Chapter 15 of this book is available open access under a CC BY 4.0 license.

Microfluidics in Cell Biology Part B  Microfluidics in Single Cells Book

Microfluidics in Cell Biology Part B Microfluidics in Single Cells


  • Author :
  • Publisher : Academic Press
  • Release Date : 2018-08-27
  • Genre: Science
  • Pages : 247
  • ISBN 10 : 9780128142837

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Microfluidics in Cell Biology Part B Microfluidics in Single Cells Book Description :

Microfluidics in Cell Biology Part B: Microfluidics in Single Cells, Volume 147, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are three sections on microfluidics in various single cell models, including microfludics in micro-organisms, microfluidics for cell culture and cell sorting of mammalian cells, and microfluidics for cell migration. Specific sections in this latest release include Temperature control and drug delivery for cell division cycle control in fission yeast H2O2 stress response in budding yeast, Antibiotic resistance in bacteria, Metabolism in bacteria, Fluidized beds for bacterial sorting and amplification, Microfluidics for cell culture and cell sorting of mammalian cells, Hydrogel microwells, Immune cells migration in complex environments, Neutrophiles migration in health and disease, Cell guidance by physical cues, Stable gradients in gels of extracellular matrix for cancer cell migration, and more. Contains contributions from experts in the field from across the world Covers a wide array of topics on both mitosis and meiosis Includes relevant, analysis based topics

Microfluidic Methods for Molecular Biology Book

Microfluidic Methods for Molecular Biology


  • Author : Chang Lu
  • Publisher : Springer
  • Release Date : 2016-05-14
  • Genre: Medical
  • Pages : 376
  • ISBN 10 : 9783319300191

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Microfluidic Methods for Molecular Biology Book Description :

This book covers the state-of-the-art research on molecular biology assays and molecular techniques enabled or enhanced by microfluidic platforms. Topics covered include microfluidic methods for cellular separations and single cell studies, droplet-based approaches to study protein expression and forensics, and microfluidic in situ hybridization for RNA analysis. Key molecular biology studies using model organisms are reviewed in detail. This is an ideal book for students and researchers in the microfluidics and molecular biology fields as well as engineers working in the biotechnology industry. This book also: Reviews exhaustively the latest techniques for single-cell genetic, epigenetic, metabolomic, and proteomic analysis Illustrates microfluidic approaches for inverse metabolic engineering, as well as analysis of circulating exosomes Broadens readers’ understanding of microfluidics convection-based PCR technology, microfluidic RNA-seq, and microfluidics for robust mobile diagnostics

Human Cell Culture Protocols Book

Human Cell Culture Protocols


  • Author : Ragai R. Mitry
  • Publisher : Humana Press
  • Release Date : 2016-08-23
  • Genre: Science
  • Pages : 435
  • ISBN 10 : 1493962086

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Human Cell Culture Protocols Book Description :

Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement. This third edition of Human Cell Culture Protocols expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues. With new chapters on pancreatic cells needed for basic studies on the pathogenesis of diabetes and for their application for islet transplantation, the book also delves into protocols for hepatocytes, skin cells, lung cells, parathyroid cells, gastric cells, renal cells, adipocytes, ovarian cells, bone cells, vascular smooth muscle cells, vascular endothelial cells, regulatory T cells, blood mononuclear cells, as well as new techniques being applied to human cell culture, particularly the use of biocompatible scaffolds to grow cells, the in vitro use of laser microdissection to isolate cells from culture, and automated cell culture. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Human Cell Culture Protocols, Third Edition makes it possible for a worker with basic cell culture training, whether in the fields of cell biology, gene therapy, and cell transplantation, to prepare cell cultures of the specific cell type necessary to forward their vital research.