Molecular Biology Techniques Book

Molecular Biology Techniques


  • Author : Sue Carson
  • Publisher : Academic Press
  • Release Date : 2012
  • Genre: Medical
  • Pages : 200
  • ISBN 10 : 9780123855442

GET BOOK
Molecular Biology Techniques Book Description :

This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Molecular Biology Techniques Book

Molecular Biology Techniques


  • Author : Walt Ream
  • Publisher : Academic Press
  • Release Date : 1998-11-17
  • Genre: Science
  • Pages : 234
  • ISBN 10 : 9780080536828

GET BOOK
Molecular Biology Techniques Book Description :

This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Key Features * Provides well-tested experimental protocols for each technique * Lists the reagents and preparation of each experiment separately * Contains a complete schedule of experiments and the preparation required * Includes study questions at the end of each chapter

Molecular Biology Techniques Book

Molecular Biology Techniques


  • Author : Sue Carson
  • Publisher : Academic Press
  • Release Date : 2019-03-05
  • Genre: Science
  • Pages : 294
  • ISBN 10 : 9780128157756

GET BOOK
Molecular Biology Techniques Book Description :

Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition is a must-have collection of methods and procedures on how to create a single, continuous, comprehensive project that teaches students basic molecular techniques. It is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology—or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students will gain hands-on experience on subcloning a gene into an expression vector straight through to the purification of the recombinant protein. Presents student-tested labs proven successful in real classroom laboratories Includes a test bank on a companion website for additional testing and practice Provides exercises that simulate a cloning project that would be performed in a real research lab Includes a prep-list appendix that contains necessary recipes and catalog numbers, providing staff with detailed instructions

Morphology Methods Book

Morphology Methods


  • Author : Ricardo V. Lloyd
  • Publisher : Springer Science & Business Media
  • Release Date : 2001-06-15
  • Genre: Medical
  • Pages : 422
  • ISBN 10 : 9781592591909

GET BOOK
Morphology Methods Book Description :

The past several decades have witnessed an impressive array of conceptual and techno logical advances in the biomedical sciences. Much of the progress in this area has developed directly as a result of new morphology-based methods that have permitted the assessment of chemical, enzymatic, immunological, and molecular parameters at the cellular and tissue levels. Additional novel approaches including laser capture microdissection have also emerged for the acquisition of homogeneous cell popula tions for molecular analyses. These methodologies have literally reshaped the approaches to fundamental biological questions and have also had a major impact in the area of diagnostic pathology. Much of the groundwork for the development of morphological methods was estab th lished in the early part of the 19 century by Francois-Vincent Raspail, generally acknowledged as the founder of the science of histochemistry. The earliest work in the field was primarily in the hands of botanists and many of the approaches to the under standing of the chemical composition of cells and tissues involved techniques such as microincineration, which destroyed structural integrity. The development of aniline th dyes in the early 20 century served as a major impetus to studies of the structural rather than chemical composition of tissue. Later in the century, however, the focus returned to the identification of chemical constituents in the context of intact cell and tissue structure.

Techniques for Molecular Biology Book

Techniques for Molecular Biology


  • Author : D Tagu
  • Publisher : Science Publishers
  • Release Date : 2006-01-04
  • Genre: Science
  • Pages : 227
  • ISBN 10 : 1578083613

GET BOOK
Techniques for Molecular Biology Book Description :

With the underpinning role of forage legumes in the nitrogen economy and animal productivity from temperate grasslands certain to expand in the future, particularly in regions where their potential has not yet been realized, it is essential that the wealth of information currently available is widely disseminated. This book serves the purpose with very detailed information on and illustrations of 35 selected forage legume species that will contribute to more efficient and viable grassland farming.

Principles and Techniques of Biochemistry and Molecular Biology Book

Principles and Techniques of Biochemistry and Molecular Biology


  • Author : Keith Wilson
  • Publisher : Cambridge University Press
  • Release Date : 2010-03-04
  • Genre: Science
  • Pages :
  • ISBN 10 : 9781107393493

GET BOOK
Principles and Techniques of Biochemistry and Molecular Biology Book Description :

This best-selling undergraduate textbook provides an introduction to key experimental techniques from across the biosciences. It uniquely integrates the theories and practices that drive the fields of biology and medicine, comprehensively covering both the methods students will encounter in lab classes and those that underpin recent advances and discoveries. Its problem-solving approach continues with worked examples that set a challenge and then show students how the challenge is met. New to this edition are case studies, for example, that illustrate the relevance of the principles and techniques to the diagnosis and treatment of individual patients. Coverage is expanded to include a section on stem cells, chapters on immunochemical techniques and spectroscopy techniques, and additional chapters on drug discovery and development, and clinical biochemistry. Experimental design and the statistical analysis of data are emphasised throughout to ensure students are equipped to successfully plan their own experiments and examine the results obtained.

Basic Techniques in Molecular Biology Book

Basic Techniques in Molecular Biology


  • Author : Stefan Surzycki
  • Publisher : Springer Science & Business Media
  • Release Date : 2012-12-06
  • Genre: Science
  • Pages : 434
  • ISBN 10 : 9783642569685

GET BOOK
Basic Techniques in Molecular Biology Book Description :

This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.

Molecular Biology Techniques Book

Molecular Biology Techniques


  • Author : Walt Ream
  • Publisher : Elsevier
  • Release Date : 1999
  • Genre: Medical
  • Pages : 234
  • ISBN 10 : 9780125839907

GET BOOK
Molecular Biology Techniques Book Description :

This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Key Features * Provides well-tested experimental protocols for each technique * Lists the reagents and preparation of each experiment separately * Contains a complete schedule of experiments and the preparation required * Includes study questions at the end of each chapter

Wood Formation in Trees Book

Wood Formation in Trees


  • Author : Nigel Chaffey
  • Publisher : CRC Press
  • Release Date : 2019-10-29
  • Genre:
  • Pages : 384
  • ISBN 10 : 0367396408

GET BOOK
Wood Formation in Trees Book Description :

Trees are a major component of the biosphere and have played an important part in the world's history and culture. With the modern challenges of global warming and dwindling fossil fuel reserves, trees, and in particular their wood, can provide solutions. Unfortunately, too little is known about the biology of these plants, due largely to a lack of appropriate techniques. In recognition of this, Wood Formation in Trees presents a variety of detailed techniques and protocols for the study of the cell and molecular biology of wood formation in trees. Internationally recognized experts, most of whom are the researchers who developed the techniques, speak with authority in this volume, and also provide first-hand tips and trade secrets to help the uninitiated master the techniques. The techniques reflect a hierarchical approach to the study of the developmental biology of wood formation: anatomical, biochemical and molecular-genetic. Trees are a tremendous but vastly under-appreciated natural resource. In an age where the natural product is so often modified to suit modern tastes and industrial processes, it is essential to understand how the natural product is made. The techniques in this book provide that essential information about the process of wood formation in trees.

Wilson and Walker s Principles and Techniques of Biochemistry and Molecular Biology Book

Wilson and Walker s Principles and Techniques of Biochemistry and Molecular Biology


  • Author : Andreas Hofmann
  • Publisher : Cambridge University Press
  • Release Date : 2018-03-31
  • Genre: Medical
  • Pages : 962
  • ISBN 10 : 9781107162273

GET BOOK
Wilson and Walker s Principles and Techniques of Biochemistry and Molecular Biology Book Description :

A major update of a best-selling textbook that introduces students to the key experimental and analytical techniques underpinning life science research.

Basic Techniques in Biochemistry and Molecular Biology Book

Basic Techniques in Biochemistry and Molecular Biology


  • Author : R. K. Sharma
  • Publisher : I. K. International Pvt Ltd
  • Release Date : 2008-11-15
  • Genre: Biochemistry
  • Pages : 280
  • ISBN 10 : 9788190746267

GET BOOK
Basic Techniques in Biochemistry and Molecular Biology Book Description :

Fundamentals of biochemistry and molecular biology is an important component of all disciplines of Biology. In the era of multidisciplinary approach, the basic techniques in Biochemistry and Molecular Biology are much needed by the students of Botany, Zoology, Microbiology, Biotechnology, Fisheries, Veterinary, Pharmacology, Physiology, Medicine, Genetics, Agriculture and allied subjects both at undergraduate and postgraduate levels. This book includes 15 chapters covering more than 135 experimental protocols. It discussed all the relevant topics like pH and buffers, spectrophotometry, chromatography, carbohydrates, lipids, proteins, electrophoresis, enzyme immunology, vitamins and pigments, metabolites and molecular biology. It includes a wide range of experiments from preparation of culture media to PCR, Southern and Western blotting. All the experiments have been meticulously designed and special care has been taken to the safety in laboratory and precautions are given wheresoever required.

Techniques in Molecular Biology Book

Techniques in Molecular Biology


  • Author : J.M. Walker
  • Publisher : Springer Science & Business Media
  • Release Date : 2012-12-06
  • Genre: Science
  • Pages : 335
  • ISBN 10 : 9789401165631

GET BOOK
Techniques in Molecular Biology Book Description :

The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly introduced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have contributed a wide range of new techniques. The purpose of this book therefore is to introduce the reader to a selection of the more advanced analytical and preparative techniques which the editors consider to be frequently used by research workers in the field of molecular biology. In choosing techniques for this book we have obviously had to be selective, and for the sake of brevity a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new terminology, a glossary of terms has been included. The techniques chosen for this book are essentially based on those used in a series of workshops on 'techniques in molecular biology' that have been held at The Hatfield Polytechnic in recent years. In choosing these chapters we have taken into account many useful suggestions and observations made by participants at these workshops. Each chapter aims to describe both the theory and relevant practical details for a given technique, and to identify both the potential and limitations of the technique. Each chapter is written by authors who regularly use the technique in their own laboratories.

Techniques in Molecular Medicine Book

Techniques in Molecular Medicine


  • Author : Friedhelm Hildebrandt
  • Publisher : Springer Science & Business Media
  • Release Date : 2013-11-11
  • Genre: Science
  • Pages : 387
  • ISBN 10 : 9783642598111

GET BOOK
Techniques in Molecular Medicine Book Description :

This manual not only provides reliable, up-to-date protocols for lab use but also the theoretical background of molecular biology, allowing users to better understand the principles underlying these techniques. It covers a wide range of methods, including the purification of nucleic acids, enzymatic modification of DNA, isolation of specific DNA fragments, PCR, cloning techniques, and gene expression. A Springer Lab Manual

Advances in Molecular Techniques Book

Advances in Molecular Techniques


  • Author : Rakesh S. Sengar
  • Publisher : CRC Press
  • Release Date : 2018-05-11
  • Genre: Medical
  • Pages : 436
  • ISBN 10 : 9781351021203

GET BOOK
Advances in Molecular Techniques Book Description :

Molecular genetics aims to comprehend biological activity at the gene sub-level. Scientists from different areas of research and applied science can use the standard techniques optimized by molecular biologists.This book serves as a guide that introduces classic molecular biology techniques and advances in molecular and genetic engineering.

Analytical Techniques in Biochemistry and Molecular Biology Book

Analytical Techniques in Biochemistry and Molecular Biology


  • Author : Rajan Katoch
  • Publisher : Springer Science & Business Media
  • Release Date : 2011-07-19
  • Genre: Science
  • Pages : 441
  • ISBN 10 : 1441997857

GET BOOK
Analytical Techniques in Biochemistry and Molecular Biology Book Description :

Advances in biochemistry now allow us to control living systems in ways that were undreamt of a decade ago. This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.

Molecular Biology Book

Molecular Biology


  • Author : Susan J. Karcher
  • Publisher : Academic Press
  • Release Date : 1995-11-28
  • Genre: Science
  • Pages : 280
  • ISBN 10 : 0080536832

GET BOOK
Molecular Biology Book Description :

This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented. Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning. Includes a glossary of over 200 terms important for understanding molecular biology Uses an inexpensive source of eukaryotic cells - great for schools on a budget Includes Methods Locator that provides instant access to the latest methods Contain clearly written, easy-to-follow, student-tested instructions: Sterile techniques Phage titration Gel electrophoresis of DNA Restriction enzyme digestion Plasmid isolation Transformation of E. Coli Recombinant DNA cloning Nick translation labeling Nonradioactive primer labelling Nonradioactive DNA detection Southern blotting Colony hybridization Purification of plant DNA RNA purification Northern blotting Purification of poly A+ RNA Polymerase chain reaction (PCR)

Basic Methods in Molecular Biology Book

Basic Methods in Molecular Biology


  • Author : Leonard Davis
  • Publisher : Elsevier
  • Release Date : 2012-12-02
  • Genre: Science
  • Pages : 399
  • ISBN 10 : 9780444601490

GET BOOK
Basic Methods in Molecular Biology Book Description :

Basic Methods in Molecular Biology discusses the heart of the most recent revolution in biology—the development of the technology of genetics. The achievements in this field have simply changed what biologists do and, perhaps even more important, the way they think. Moreover, never before have scientists from such a broad range of disciplines rushed into such a small and slightly arcane field to learn and carry off a bit of the technology. This book comprises 21 chapters, opening with three introductory ones that discuss the basics of molecular biology; the tools of the molecular biologist; and general preparations, procedures, and considerations for use of the book. The following chapters then discuss cloning vectors and bacterial cells; preparation of DNA from eukaryotic cells; probing nucleic acids; plasmid DNA preparation; DNA restriction fragment preparation; purification of DNA; and preparation and analysis of RNA from eukaryotic cells. Other chapters cover preparation of DNA from bacteriophage clones; cloning DNA from the eukaryotic genome; subcloning into plasmids; M13 cloning and sequencing; further characterization of cloned DNA; transfection of mammalian cells in culture; protein methods; general methods; and specialized methods. This book will be of interest to practitioners in the fields of biology and molecular genetics.

Oral Biology  Molecular Techniques and Applications Book

Oral Biology Molecular Techniques and Applications


  • Author : Gregory J. Seymour
  • Publisher : Methods in Molecular Biology
  • Release Date : 2018-12-21
  • Genre: Medical
  • Pages : 520
  • ISBN 10 : 1493982729

GET BOOK
Oral Biology Molecular Techniques and Applications Book Description :

This second edition volume presents a selection of cellular and molecular techniques that can be adapted to cover a range of applications and diseases. The book is divided into three sections: saliva and oral diseases, molecular biosciences, and cell and tissues. The first section contains chapters that discuss proteomic analyses by mass spectrometry and NMR-based metabolomics that can be used to not only study saliva, but also to assess other oral fluids such as gingival crevicular fluid. The second section contains chapters that profile oral microbial communities, quantitative real-time PCR, and adhesion of yeast and bacteria to oral surfaces. The third section deals with a range of approaches that enable the behavior of cells and tissues in both health and disease to be analyzed at the molecular level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Oral Biology: Molecular Techniques and Applications, Second Edition is a useful resource for oral biologists at all levels (students, early career researchers, and experienced veterans), and it provides a ready reference to new techniques and approaches that can be used to answer numerous specific scientific questions that may lead to a deeper understanding and treatments of oral diseases.

Pattern Recognition in Computational Molecular Biology Book

Pattern Recognition in Computational Molecular Biology


  • Author : Mourad Elloumi
  • Publisher : John Wiley & Sons
  • Release Date : 2015-12-24
  • Genre: Technology & Engineering
  • Pages : 656
  • ISBN 10 : 9781119078869

GET BOOK
Pattern Recognition in Computational Molecular Biology Book Description :

A comprehensive overview of high-performance pattern recognition techniques and approaches to Computational Molecular Biology This book surveys the developments of techniques and approaches on pattern recognition related to Computational Molecular Biology. Providing a broad coverage of the field, the authors cover fundamental and technical information on these techniques and approaches, as well as discussing their related problems. The text consists of twenty nine chapters, organized into seven parts: Pattern Recognition in Sequences, Pattern Recognition in Secondary Structures, Pattern Recognition in Tertiary Structures, Pattern Recognition in Quaternary Structures, Pattern Recognition in Microarrays, Pattern Recognition in Phylogenetic Trees, and Pattern Recognition in Biological Networks. Surveys the development of techniques and approaches on pattern recognition in biomolecular data Discusses pattern recognition in primary, secondary, tertiary and quaternary structures, as well as microarrays, phylogenetic trees and biological networks Includes case studies and examples to further illustrate the concepts discussed in the book Pattern Recognition in Computational Molecular Biology: Techniques and Approaches is a reference for practitioners and professional researches in Computer Science, Life Science, and Mathematics. This book also serves as a supplementary reading for graduate students and young researches interested in Computational Molecular Biology.

Algorithms in Computational Molecular Biology Book

Algorithms in Computational Molecular Biology


  • Author : Mourad Elloumi
  • Publisher : John Wiley & Sons
  • Release Date : 2011-04-04
  • Genre: Science
  • Pages : 1080
  • ISBN 10 : 9781118101988

GET BOOK
Algorithms in Computational Molecular Biology Book Description :

This book represents the most comprehensive and up-to-date collection of information on the topic of computational molecular biology. Bringing the most recent research into the forefront of discussion, Algorithms in Computational Molecular Biology studies the most important and useful algorithms currently being used in the field, and provides related problems. It also succeeds where other titles have failed, in offering a wide range of information from the introductory fundamentals right up to the latest, most advanced levels of study.