Site Specific and Global Epigenomic Editing Book

Site Specific and Global Epigenomic Editing


  • Author : Feng C Zhou
  • Publisher : Academic Press
  • Release Date : 2022-06-15
  • Genre: Medical
  • Pages : 704
  • ISBN 10 : 0128193980

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Site Specific and Global Epigenomic Editing Excerpt :

In recent years, alongside the largely popularized advances in human genome editing and CRISPR-cas9-driven therapeutics, epigenomic editing has made strong strides, though a clear understanding is still forming in the field of the exact role that epigenetics play in pathogenesis, the full range of sites for possible epigenetic modification, and the potential to target these sites to modulate disease progression. Key questions for researchers embarking in this field include: Which of the hundreds of epigenomic marks and features are relevant, and which of those are likely to serve a role in regulating gene expression, cell behavior, and phenotypic outcomes? This volume is the first to provide a thorough, conceptual introduction to the field of epigenetic regulation and a thorough accounting of sites for possible epigenetic edits and disease modulation. Protocols, and applied case studies examine early disease targets and likely pathways of therapeutic significance, while also outlining common challenges in the field, and the addressing the range of bioethical questions that come with altering phenotypes. This book addresses its topic in a comprehensive, but accessible manner, highlighting practical tools and burning questions remaining in the field to enable fresh research. Offers a full, conceptual and applied framework for implementing epigenomic editing in biological research and drug discovery Discusses fundamental theory, experimental design, and applied approaches alongside common research challenges and bioethical concerns Examines epigenomic editing's potential role in modulation of developmental, neuropsychiatric, and immunological disorders, as well as viral infections and cancer

Chromatin and Epigenetics Book

Chromatin and Epigenetics


  • Author : Colin Logie
  • Publisher : BoD – Books on Demand
  • Release Date : 2020-01-29
  • Genre: Science
  • Pages : 294
  • ISBN 10 : 9781789844924

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Chromatin and Epigenetics Excerpt :

Genomics has gathered broad public attention since Lamarck put forward his top-down hypothesis of 'motivated change' in 1809 in his famous book "Philosophie Zoologique" and even more so since Darwin published his famous bottom-up theory of natural selection in "The Origin of Species" in 1859. The public awareness culminated in the much anticipated race to decipher the sequence of the human genome in 2002. Over all those years, it has become apparent that genomic DNA is compacted into chromatin with a dedicated 3D higher-order organization and dynamics, and that on each structural level epigenetic modifications exist. The book "Chromatin and Epigenetics" addresses current issues in the fields of epigenetics and chromatin ranging from more theoretical overviews in the first four chapters to much more detailed methodologies and insights into diagnostics and treatments in the following chapters. The chapters illustrate in their depth and breadth that genetic information is stored on all structural and dynamical levels within the nucleus with corresponding modifications of functional relevance. Thus, only an integrative systems approach allows to understand, treat, and manipulate the holistic interplay of genotype and phenotype creating functional genomes. The book chapters therefore contribute to this general perspective, not only opening opportunities for a true universal view on genetic information but also being key for a general understanding of genomes, their function, as well as life and evolution in general.

Targeted Genome Editing Using Site Specific Nucleases Book

Targeted Genome Editing Using Site Specific Nucleases


  • Author : Takashi Yamamoto
  • Publisher : Springer
  • Release Date : 2015-01-05
  • Genre: Science
  • Pages : 205
  • ISBN 10 : 9784431552277

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Targeted Genome Editing Using Site Specific Nucleases Excerpt :

This book serves as an introduction to targeted genome editing, beginning with the background of this rapidly developing field and methods for generation of engineered nucleases. Applications of genome editing tools are then described in detail, in iPS cells and diverse organisms such as mice, rats, marine invertebrates, fish, frogs, and plants. Tools that are mentioned include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9, all of which have received much attention in recent years as breakthrough technologies. Genome editing with engineered nucleases allows us to precisely change the target genome of living cells and is a powerful way to control functional genes. It is feasible in almost all organisms ranging from bacteria to plants and animals, as well as in cultured cells such as ES and iPS cells. Various genome modifications have proven successful, including gene knockout and knock-in experiments with targeting vectors and chromosomal editing. Genome editing technologies hold great promise for the future, for example in biomedical research, clinical medicine, and generation of crops and livestock with desirable traits. A wide range of readers will find this book interesting, and with its focus on applications in a variety of organisms and cells, the book will be valuable for life scientists in all fields.

Quantitative Genetics  Genomics and Plant Breeding  2nd Edition Book

Quantitative Genetics Genomics and Plant Breeding 2nd Edition


  • Author : Manjit S. Kang
  • Publisher : CABI
  • Release Date : 2020-04-01
  • Genre: Science
  • Pages : 431
  • ISBN 10 : 9781789240214

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Quantitative Genetics Genomics and Plant Breeding 2nd Edition Excerpt :

This book presents state-of-the-art, authoritative chapters on contemporary issues in the broad areas of quantitative genetics, genomics and plant breeding. Section 1 (Chapters 2 to 12) emphasizes the application of genomics, and genome and epigenome editing techniques, in plant breeding; bioinformatics; quantitative trait loci mapping; and the latest approaches of examining and exploiting genotype-environment interactions. Section 2 (Chapters 13 to 20) represents the intersection of breeding, genetics and genomics. This section describes the use of cutting-edge molecular breeding and quantitative genetics techniques in wheat, rice, maize, root and tuber crops and pearl millet. Overall, the book focuses on using genomic information to help evaluate traits that can combat biotic/abiotic stresses, genome-wide association mapping, high-throughput genotyping/phenotyping, biofortification, use of big data, orphan crops, and gene editing techniques. The examples featured are taken from across crop science research and cover a wide geographical base.

Epigenetics Methods Book

Epigenetics Methods


  • Author : Trygve Tollefsbol
  • Publisher : Academic Press
  • Release Date : 2020-07-08
  • Genre: Medical
  • Pages : 736
  • ISBN 10 : 9780128194157

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Epigenetics Methods Excerpt :

In recent years, the field of epigenetics has grown significantly, driving new understanding of human developmental processes and disease expression, as well as advances in diagnostics and therapeutics. As the field of epigenetics continues to grow, methods and technologies have multiplied, resulting in a wide range of approaches and tools researchers might employ. Epigenetics Methods offers comprehensive instruction in methods, protocols, and experimental approaches applied in field of epigenetics. Here, across thirty-five chapters, specialists offer step-by-step overviews of methods used to study various epigenetic mechanisms, as employed in basic and translational research. Leading the reader from fundamental to more advanced methods, the book begins with thorough instruction in DNA methylation techniques and gene or locus-specific methylation analyses, followed by histone modification methods, chromatin evaluation, enzyme analyses of histone methylation, and studies of non-coding RNAs as epigenetic modulators. Recently developed techniques and technologies discussed include single-cell epigenomics, epigenetic editing, computational epigenetics, systems biology epigenetic methods, and forensic epigenetic approaches. Epigenetics methods currently in-development, and their implication for future research, are also considered in-depth. In addition, as with the wider life sciences, reproducibility across experiments, labs, and subdisciplines is a growing issue for epigenetics researchers. This volume provides consensus-driven methods instruction and overviews. Tollefsbol and contributing authors survey the range of existing methods; identify best practices, common themes, and challenges; and bring unity of approach to a diverse and ever-evolving field. Includes contributions by leading international investigators involved in epigenetic research and clinical and therapeutic application Integrates technology and translation with fundamental chapters on epigenetics meth

DNA Methyltransferases   Role and Function Book

DNA Methyltransferases Role and Function


  • Author : Albert Jeltsch
  • Publisher : Springer
  • Release Date : 2016-11-08
  • Genre: Science
  • Pages : 535
  • ISBN 10 : 9783319436241

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DNA Methyltransferases Role and Function Excerpt :

DNA methyltransferases are important enzymes in a broad range of organisms. Dysfunction of DNA methyltransferases in humans leads to many severe diseases, including cancer. This book focuses on the biochemical properties of these enzymes, describing their structures and mechanisms in bacteria, humans and other species, including plants, and also explains the biological processes of reading of DNA methylation and DNA demethylation. It covers many emerging aspects of the biological roles of DNA methylation functioning as an essential epigenetic mark and describes the role of DNA methylation in diseases. Moreover, the book explains modern technologies, like targeted rewriting of DNA methylation by designed DNA methyltransferases, as well as technological applications of DNA methyltransferases in DNA labelling. Finally, the book summarizes recent methods for the analysis of DNA methylation in human DNA. Overall, this book represents a comprehensive state-of-the-art- work and is a must-have for advanced researchers in the field of DNA methylation and epigenetics.

Reprogramming the Genome  CRISPR Cas based Human Disease Therapy Book

Reprogramming the Genome CRISPR Cas based Human Disease Therapy


  • Author : Anonim
  • Publisher : Academic Press
  • Release Date : 2021-06-12
  • Genre: Science
  • Pages : 390
  • ISBN 10 : 9780323853248

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Reprogramming the Genome CRISPR Cas based Human Disease Therapy Excerpt :

Reprogramming the Genome: CRISPR-Cas-based Human Disease Therapy, presents the collation of chapters written by eminent scientists worldwide. CRISPR-Cas9 is a key technology for targeted genome editing and regulation in a number of organisms including mammalian cells. It is a rapid, simple, and cost-effective solution. CRISPR-Cas system has recently gained much scientific and public attention. This volume covers CRISPR-Cas9 based mammalian genome editing, creating disease models, cancer therapy, neurological, heredity, blood disorders, defective gene correction, stem cells therapy, epigenetic modifications, patents, ethics, biosafety and regulatory issues challenges and opportunities. This book is a key source of information on mammalian genome editing available in a single volume. This book will be useful for beginners in mammalian genome editing and also students, researchers, scientists, policymakers, clinicians and stakeholders interested in genome editing in several areas. Offers basic understanding and a clear picture of mammalian genome editing through CRISPR-Cas systems Discusses how to create mammalian disease models, stem cell modification, epigenetic modifications, correction of defective gene in blood disorders, heredity, neurological disorders and many more Discusses the application of CRISPR-Cas9 systems in basic sciences, biomedicine, molecular biology, translational sciences, neurobiology, neurology, cancer, stem cells, and many more

CRISPR Genome Surgery in Stem Cells and Disease Tissues Book

CRISPR Genome Surgery in Stem Cells and Disease Tissues


  • Author : Stephen H. Tsang
  • Publisher : Academic Press
  • Release Date : 2021-10-20
  • Genre: Science
  • Pages : 136
  • ISBN 10 : 9780128178775

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CRISPR Genome Surgery in Stem Cells and Disease Tissues Excerpt :

CRISPR Genome Surgery in Stem Cells and Disease Tissues focuses uniquely on the clinical applications of CRISPR/Cas9 based technology. Topics include the latest advances in gene editing and its translational applications to various diseases, including retinal degenerative disease, recessively inherited diseases, and dominantly inherited diseases, to name a few. The book's target audience includes researchers, students, clinicians and the general public. This space that is not currently served by any existing resource, so this publication fills a gap in current literature. Provides a thorough review of CRISPR-Cas9, from discovery to therapy Covers the latest advances in gene editing and its translational applications to various diseases Written by global leaders in the fields of gene editing and stem cell therapy

Computational Epigenetics and Diseases Book

Computational Epigenetics and Diseases


  • Author : Anonim
  • Publisher : Academic Press
  • Release Date : 2019-02-06
  • Genre: Business & Economics
  • Pages : 450
  • ISBN 10 : 9780128145142

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Computational Epigenetics and Diseases Excerpt :

Computational Epigenetics and Diseases, written by leading scientists in this evolving field, provides a comprehensive and cutting-edge knowledge of computational epigenetics in human diseases. In particular, the major computational tools, databases, and strategies for computational epigenetics analysis, for example, DNA methylation, histone modifications, microRNA, noncoding RNA, and ceRNA, are summarized, in the context of human diseases. This book discusses bioinformatics methods for epigenetic analysis specifically applied to human conditions such as aging, atherosclerosis, diabetes mellitus, schizophrenia, bipolar disorder, Alzheimer disease, Parkinson disease, liver and autoimmune disorders, and reproductive and respiratory diseases. Additionally, different organ cancers, such as breast, lung, and colon, are discussed. This book is a valuable source for graduate students and researchers in genetics and bioinformatics, and several biomedical field members interested in applying computational epigenetics in their research. Provides a comprehensive and cutting-edge knowledge of computational epigenetics in human diseases Summarizes the major computational tools, databases, and strategies for computational epigenetics analysis, such as DNA methylation, histone modifications, microRNA, noncoding RNA, and ceRNA Covers the major milestones and future directions of computational epigenetics in various kinds of human diseases such as aging, atherosclerosis, diabetes, heart disease, neurological disorders, cancers, blood disorders, liver diseases, reproductive diseases, respiratory diseases, autoimmune diseases, human imprinting disorders, and infectious diseases

Genetics of Human Infertility Book

Genetics of Human Infertility


  • Author : P.H. Vogt
  • Publisher : Karger Medical and Scientific Publishers
  • Release Date : 2017-09-12
  • Genre: Medical
  • Pages : 178
  • ISBN 10 : 9783318060980

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Genetics of Human Infertility Excerpt :

Infertility affects more than one in ten couples worldwide and is related to highly heterogeneous pathologies sometimes only discernible in the germ line. Its complex etiology often, but not always, includes genetic factors besides anatomical defects, immunological interference, and environmental aspects. Nearly 30% of infertility cases are probably caused only by genetic defects. Thereby experimental animal knockout models convincingly show that infertility can be caused by single or multiple gene defects. Translating those basic research findings into clinical studies is challenging, leaving genetic causes for the vast majority of infertility patients unexplained. Nevertheless, a large number of candidate genes have been revealed by sophisticated molecular methods. This book provides a comprehensive overview on the subject of infertility written by the leading authorities in this field. It covers topics including basic biological, cytological, and molecular studies, as well as common and uncommon syndromes. It is a must-read for human geneticists, endocrinologists, epidemiologists, zoologists, and counsellors in human genetics, infertility, and assisted reproduction.

Neuroepigenomics in Aging and Disease Book

Neuroepigenomics in Aging and Disease


  • Author : Raul Delgado-Morales
  • Publisher : Springer
  • Release Date : 2017-05-18
  • Genre: Science
  • Pages : 525
  • ISBN 10 : 9783319538891

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Neuroepigenomics in Aging and Disease Excerpt :

Epigenetic mechanisms (DNA modifications, histone alterations and non-coding RNAs) are crucial for transcriptional regulation and alterations of the “physiological epigenome” are increasingly associated with human diseases. During the last decade the emerging field of neuroepigenomics have started to impact tremendously in areas such learning and memory, addiction or neurodegeneration. This expert volume covers the role of epigenetic molecular mechanism in regulation of central nervous system’s function, one of the most exciting areas of contemporary molecular neuroscience. The book describes the current knowledge on the epigenetic basis of human disease covering the complete lifespan: from neurodevelopment/childhood (Rett Syndrome, Rubinstein-Taybi, autism), adolescence (eating disorders, drug addiction, anxiety), adulthood (depression, schizophrenia, amyotrophic lateral sclerosis, Huntington’s disease) and elderly (Alzheimer’s disease, Parkinson’s disease). The book also covers the three major players on neuroepigenomic mechanisms: histones alterations, DNA modifications and non-coding RNAs, their roles at the molecular and cellular level and the impact of their alterations on neuronal function and behavior. Finally, a special chapter on state-of-the-art technologies helps the reader not only to understand epigenetic driven changes in human cognition and diseases but also the methodology that will help to generate paradigm shifts on our understanding of brain function and the role of the neuroepigenome in human diseases.

Mutation Specific Gene Editing for Blood Disorders Book

Mutation Specific Gene Editing for Blood Disorders


  • Author : Carsten Werner Lederer
  • Publisher : Frontiers Media SA
  • Release Date : 2021-11-25
  • Genre: Science
  • Pages : null
  • ISBN 10 : 9782889716883

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Mutation Specific Gene Editing for Blood Disorders Excerpt :

Dr. Miccio holds patents related to viral gene delivery vectors. The Topic Editors acknowledge the use of image material from kindpng.com and from Crystal and Annie Spratt on unsplash.com.

Molecular Breeding in Wheat  Maize and Sorghum Book

Molecular Breeding in Wheat Maize and Sorghum


  • Author : Mohammad Anwar Hossain
  • Publisher : CABI
  • Release Date : 2021-06-30
  • Genre: Technology & Engineering
  • Pages : 550
  • ISBN 10 : 9781789245431

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Molecular Breeding in Wheat Maize and Sorghum Excerpt :

The global population is projected to reach almost 10 billion by 2050, and food and feed production will need to increase by 70%. Wheat, maize and sorghum are three key cereals which provide nutrition for the majority of the world's population. Their production is affected by various abiotic stresses which cause significant yield losses. The effects of climate change also increase the frequency and severity of such abiotic stresses. Molecular breeding technologies offer real hope for improving crop yields. Although significant progress has been made over the last few years, there is still a need to bridge the large gap between yields in the most favorable and most stressful conditions.

Epigenetic Gene Expression and Regulation Book

Epigenetic Gene Expression and Regulation


  • Author : Suming Huang
  • Publisher : Academic Press
  • Release Date : 2015-10-19
  • Genre: Science
  • Pages : 482
  • ISBN 10 : 9780128004715

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Epigenetic Gene Expression and Regulation Excerpt :

Epigenetic Gene Expression and Regulation reviews current knowledge on the heritable molecular mechanisms that regulate gene expression, contribute to disease susceptibility, and point to potential treatment in future therapies. The book shows how these heritable mechanisms allow individual cells to establish stable and unique patterns of gene expression that can be passed through cell divisions without DNA mutations, thereby establishing how different heritable patterns of gene regulation control cell differentiation and organogenesis, resulting in a distinct human organism with a variety of differing cellular functions and tissues. The work begins with basic biology, encompasses methods, cellular and tissue organization, topical issues in epigenetic evolution and environmental epigenesis, and lastly clinical disease discovery and treatment. Each highly illustrated chapter is organized to briefly summarize current research, provide appropriate pedagogical guidance, pertinent methods, relevant model organisms, and clinical examples. Reviews current knowledge on the heritable molecular mechanisms that regulate gene expression, contribute to disease susceptibility, and point to potential treatment in future therapies Helps readers understand how epigenetic marks are targeted, and to what extent transgenerational epigenetic changes are instilled and possibly passed onto offspring Chapters are replete with clinical examples to empower the basic biology with translational significance Offers more than 100 illustrations to distill key concepts and decipher complex science