Structural Biology Using Electrons and X rays Book

Structural Biology Using Electrons and X rays


  • Author : Michael F Moody
  • Publisher : Academic Press
  • Release Date : 2011-03-03
  • Genre: Science
  • Pages : 450
  • ISBN 10 : 0080919456

DOWNLOAD BOOK
Structural Biology Using Electrons and X rays Excerpt :

Structural Biology Using Electrons and X-Rays discusses the diffraction and image-based methods used for the determination of complex biological macromolecules. The book focuses on the Fourier transform theory, which is a mathematical function that is computed to transform signals between time and frequency domain. Composed of five parts, the book examines the development of nuclear magnetic resonance (NMR), which allows the calculation of the images of a certain protein. Parts 1 to 4 provide the basic information and the applications of Fourier transforms, as well as the different methods used for image processing using X-ray crystallography and the analysis of electron micrographs. Part 5 focuses entirely on the mathematical aspect of Fourier transforms. In addition, the book examines detailed structural analyses of a specimen’s symmetry (i.e., crystals, helices, polyhedral viruses and asymmetrical particles). This book is intended for the biologist or biochemist who is interested in different methods and techniques for calculating the images of proteins using nuclear magnetic resonance (NMR). It is also suitable for readers without a background in physical chemistry or mathematics. Emphasis on common principles underlying all diffraction-based methods Thorough grounding in theory requires understanding of only simple algebra Visual representations and explanations of challenging content Mathematical detail offered in short-course form to parallel the text

X ray Free Electron Lasers Book

X ray Free Electron Lasers


  • Author : Sébastien Boutet
  • Publisher : Springer
  • Release Date : 2018-12-27
  • Genre: Technology & Engineering
  • Pages : 479
  • ISBN 10 : 9783030005511

DOWNLOAD BOOK
X ray Free Electron Lasers Excerpt :

The timely volume describes recent discoveries and method developments that have revolutionized Structural Biology with the advent of X-ray Free Electron Lasers. It provides, for the first time, a comprehensive examination of this cutting-edge technology. It discusses of-the-moment topics such as growth and detection of nanocrystals, Sample Delivery Techniques for serial femtosecond crystallography, data collection methods at XFELs, and more. This book aims to provide the readers with an overview of the new methods that have been recently developed as well as a prospective on new methods under development. It highlights the most important and novel Structural Discoveries made recently with XFELS, contextualized with a big-picture discussion of future developments.

The Resolution Revolution  Recent Advances In cryoEM Book

The Resolution Revolution Recent Advances In cryoEM


  • Author : Anonim
  • Publisher : Academic Press
  • Release Date : 2016-08-26
  • Genre: Medical
  • Pages : 486
  • ISBN 10 : 9780128054352

DOWNLOAD BOOK
The Resolution Revolution Recent Advances In cryoEM Excerpt :

cryoEM, a new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods and new developments in recording images, the creation, evaluation and validation of 3D maps from the images, model building into maps and refinement of the resulting atomic structures, and applications of essentially single particle methods to helical structures and to sub-tomogram averaging. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods that determine the structures of biological molecules, a vital step for understanding their function Contains the technical developments underpinning the advances of cryoEM and captures the exciting insights that have resulted

Architects of Structural Biology Book

Architects of Structural Biology


  • Author : John Meurig Thomas
  • Publisher : Oxford University Press, USA
  • Release Date : 2020-02-28
  • Genre: Nature
  • Pages : 320
  • ISBN 10 : 9780198854500

DOWNLOAD BOOK
Architects of Structural Biology Excerpt :

This is a history of the personalities and single-minded devotion of four Nobel laureates who played a pivotal role in the creation of a new and prevalent branch of biology. This led to major medical advances in one of the greatest centres of scientific research: the Laboratory of Molecular Biology in Cambridge, which they helped to establish.

Membrane Structural Biology Book

Membrane Structural Biology


  • Author : Mary Luckey
  • Publisher : Cambridge University Press
  • Release Date : 2014-02-24
  • Genre: Science
  • Pages : 400
  • ISBN 10 : 9781107729339

DOWNLOAD BOOK
Membrane Structural Biology Excerpt :

This textbook provides a strong foundation and a clear overview for students of membrane biology and an invaluable synthesis of cutting-edge research for working scientists. The text retains its clear and engaging style, providing a solid background in membrane biochemistry, while also incorporating the approaches of biophysics, genetics and cell biology to investigations of membrane structure, function and biogenesis to provide a unique overview of this fast-moving field. A wealth of new high resolution structures of membrane proteins are presented, including the Na/K pump and a receptor-G protein complex, offering exciting insights into how they function. All key tools of current membrane research are described, including detergents and model systems, bioinformatics, protein-folding methodology, crystallography and diffraction, and molecular modeling. This comprehensive and up-to-date text, emphasising the correlations between membrane research and human health, provides a solid foundation for all those working in this field.

Advances in Protein Molecular and Structural Biology Methods Book

Advances in Protein Molecular and Structural Biology Methods


  • Author : Timir Tripathi
  • Publisher : Academic Press
  • Release Date : 2022-01-14
  • Genre: Science
  • Pages : 714
  • ISBN 10 : 9780323902656

DOWNLOAD BOOK
Advances in Protein Molecular and Structural Biology Methods Excerpt :

Advances in Protein Molecular and Structural Biology Methods offers a complete overview of the latest tools and methods applicable to the study of proteins at the molecular and structural level. The book begins with sections exploring tools to optimize recombinant protein expression and biophysical techniques such as fluorescence spectroscopy, NMR, mass spectrometry, cryo-electron microscopy, and X-ray crystallography. It then moves towards computational approaches, considering structural bioinformatics, molecular dynamics simulations, and deep machine learning technologies. The book also covers methods applied to intrinsically disordered proteins (IDPs)followed by chapters on protein interaction networks, protein function, and protein design and engineering. It provides researchers with an extensive toolkit of methods and techniques to draw from when conducting their own experimental work, taking them from foundational concepts to practical application. Presents a thorough overview of the latest and emerging methods and technologies for protein study Explores biophysical techniques, including nuclear magnetic resonance, X-ray crystallography, and cryo-electron microscopy Includes computational and machine learning methods Features a section dedicated to tools and techniques specific to studying intrinsically disordered proteins

Advances in Imaging and Electron Physics Book

Advances in Imaging and Electron Physics


  • Author : Anonim
  • Publisher : Academic Press
  • Release Date : 2017-05-23
  • Genre: Science
  • Pages : 172
  • ISBN 10 : 9780128121931

DOWNLOAD BOOK
Advances in Imaging and Electron Physics Excerpt :

Advances in Imaging and Electron Physics, Volume 200, the latest release in a series that merges two long-running serials, Advances in Electronics and Electron Physics and Advances in Optical and Electron Microscopy, features extended articles on the physics of electron devices (especially semiconductor devices), particle optics at high and low energies, microlithography, image science, digital image processing, electromagnetic wave propagation, electron microscopy, and the computing methods used in all these domains. Contains contributions from leading authorities on the subject matter Informs and updates on all the latest developments in the field of imaging and electron physics Provides practitioners interested in microscopy, optics, image processing, mathematical morphology, electromagnetic fields, electron, and ion emission with a valuable resource Features extended articles on the physics of electron devices (especially semiconductor devices), particle optics at high and low energies, microlithography, image science, and digital image processing

Neutron Crystallography in Structural Biology Book

Neutron Crystallography in Structural Biology


  • Author : Anonim
  • Publisher : Academic Press
  • Release Date : 2020-02-22
  • Genre: Science
  • Pages : 408
  • ISBN 10 : 9780128192153

DOWNLOAD BOOK
Neutron Crystallography in Structural Biology Excerpt :

Neutron Crystallography in Structural Biology, Volume 634, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this updated release include Fundamentals of neutron crystallography in structural biology, Large crystal growth for neutron protein crystallography, Prospects for membrane protein crystals in NMX, IMAGINE: The neutron protein crystallography beamline at the high flux isotope reactor, The macromolecular neutron diffractometer at the spallation neutron source, Current status and near future plan of neutron protein crystallography at J-PARC, Neutron macromolecular crystallography at the European spallation source, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Includes the latest information on neutron crystallography in structural biology

Foundations of Structural Biology Book

Foundations of Structural Biology


  • Author : Leonard J. Banaszak
  • Publisher : Elsevier
  • Release Date : 2000-02-08
  • Genre: Science
  • Pages : 168
  • ISBN 10 : 0080521843

DOWNLOAD BOOK
Foundations of Structural Biology Excerpt :

Imagine trying to understand an engine without visualizing its moving parts. Biological processes involve far more complex chemical reactions and components than any engine. Furthermore, the parts work together to do many more functions than an engine which sole task is to turn a shaft. Understanding the implications of the three-dimensional coordinates for a molecule with several thousand atoms requires an understanding of, and practice with, 3D imaging. For many biologists, this means acquiring a whole new set of skills. Foundations of Structural Biology is aimed at helping the reader develop visualization skills for protein or DNA segments, while also describing the fundamental principles underlying the organization and interaction between these complex molecules. Key Features * Explains how to use coordinate databases and atomic coordinates of biological macromolecules * Teaches the skills of stereoviewing * Contains computer-generated stereographics * Describes the principles of symmetry and handedness in proteins and DNA * Introduces metal and lipid binding proteins and DNA-protein interactions * Explains the principles involved in understanding secondary and quaternary structure * Includes coverage of protein-metal, protein-nucleic acid, and protein-lipid interactions

Astroparticle  Particle and Space Physics  Detectors and Medical Physics Applications Book

Astroparticle Particle and Space Physics Detectors and Medical Physics Applications


  • Author : Anonim
  • Publisher : World Scientific
  • Release Date : 2008
  • Genre: Electronic books
  • Pages : 1060
  • ISBN 10 : 9789812819093

DOWNLOAD BOOK
Astroparticle Particle and Space Physics Detectors and Medical Physics Applications Excerpt :

The exploration of the subnuclear world is done through increasingly complex experiments covering a wide range of energies and in a large variety of environments OCo from particle accelerators and underground detectors to satellites and space laboratories. For these research programs to succeed, novel techniques, new materials and new instrumentation need to be used in detectors, often on a large scale. Hence, particle physics is at the forefront of technological advancement and leads to numerous applications. Among these, medical applications have a particular importance due to the health and social benefits they bring. This volume reviews the advances made in all technological aspects of current experiments in the field."

X Ray Free Electron Lasers Book

X Ray Free Electron Lasers


  • Author : Uwe Bergmann
  • Publisher : Royal Society of Chemistry
  • Release Date : 2017-08-11
  • Genre: Science
  • Pages : 463
  • ISBN 10 : 9781788012027

DOWNLOAD BOOK
X Ray Free Electron Lasers Excerpt :

The ultra-bright femtosecond X-ray pulses provided by X-ray free electron lasers (XFELs) open up opportunities to study the structure and dynamics of a wide variety of systems beyond what is possible with synchrotron sources. This book introduces the principles and properties of currently operating and future XFELs, before outlining applications in materials science, chemistry and biology. Edited by pioneers in this exciting field, and featuring contributions from leading researchers, this book is ideal for researchers working with XFELs, synchrotron radiation, ultrafast and femtosecond crystallography and femtosecond spectroscopy.

Stalk Structures in Lipid Bilayer Fusion Studied by X ray Diffraction Book

Stalk Structures in Lipid Bilayer Fusion Studied by X ray Diffraction


  • Author : Sebastian Aeffner
  • Publisher : Universitätsverlag Göttingen
  • Release Date : 2012
  • Genre: Uncategoriezed
  • Pages : 182
  • ISBN 10 : 3863950437

DOWNLOAD BOOK
Stalk Structures in Lipid Bilayer Fusion Studied by X ray Diffraction Excerpt :

The fusion of two biological membranes is an important step in many processes on the cellular and sub-cellular level. Understanding the involved interplay of different lipid species, a specialized protein machinery and water on length scales of few nanometers poses a significant challenge to current structural biology. Among several complementary approaches, one strategy is to study the structural rearrangements of the lipid matrix. As the initial step, lipid bilayers must be forced into close contact to form a non-bilayer intermediate termed a stalk. This has been the subject of numerous theoretical studies and simulations, but experimental data on stalks are largely lacking. Currently, the only way to obtain structural information at the required sub-nanometer resolution is x-ray diffraction on the recently discovered stalk phaseʺ formed by certain lipids. We apply this method to elucidate the effect of lipid composition on stalk geometry and the repulsive forces between lipid bilayers prior to stalk formation. An approach based on differential geometry of electron density isosurfaces is introduced to analyze the curvatures and bending energies of the lipid monolayers. For the first time, this connects experiment-based structures of stalks and the associated bending and hydration energies. In addition, this thesis aims to provide a self-contained introduction to the required background in x-ray diffraction on lipid mesophases and electron density reconstruction.

Integrative Structural Biology with Hybrid Methods Book

Integrative Structural Biology with Hybrid Methods


  • Author : Haruki Nakamura
  • Publisher : Springer
  • Release Date : 2019-01-08
  • Genre: Science
  • Pages : 272
  • ISBN 10 : 9789811322006

DOWNLOAD BOOK
Integrative Structural Biology with Hybrid Methods Excerpt :

This book presents a new emerging concept of "Integrative Structural Biology". It covers current trends of the molecular and cellular structural biology, providing new methods to observe, validate, and keep the structural models of the large cellular machines with recent scientific results. Structures of very large macromolecular machines in cells are being determined by combining observations from complementary experimental methods. Thus, this volume presents the each methods such as X-ray crystallography, NMR spectroscopy, 3DEM, small-angle scattering (SAS), FRET, crosslinking, and enables the readers to understand the hybrid methods. This book discusses how those integrative models should be represented, validated and archived. A unique highlight of this book is discussion of the data validation and archive, which are big problems in this filed along with the progress of this field. The researchers in biology will be interested in this book as a guide book for learning the current structure biology, but also those in structure biology may use this book as a comprehensive reference to cover broad topics.