Therapeutic Antibody Engineering Book

Therapeutic Antibody Engineering


  • Author : William R Strohl
  • Publisher : Elsevier
  • Release Date : 2012-10-16
  • Genre: Science
  • Pages : 696
  • ISBN 10 : 9781908818096

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Therapeutic Antibody Engineering Excerpt :

The field of antibody engineering has become a vital and integral part of making new, improved next generation therapeutic monoclonal antibodies, of which there are currently more than 300 in clinical trials across several therapeutic areas. Therapeutic antibody engineering examines all aspects of engineering monoclonal antibodies and analyses the effect that various genetic engineering approaches will have on future candidates. Chapters in the first part of the book provide an introduction to monoclonal antibodies, their discovery and development and the fundamental technologies used in their production. Following chapters cover a number of specific issues relating to different aspects of antibody engineering, including variable chain engineering, targets and mechanisms of action, classes of antibody and the use of antibody fragments, among many other topics. The last part of the book examines development issues, the interaction of human IgGs with non-human systems, and cell line development, before a conclusion looking at future issues affecting the field of therapeutic antibody engineering. Goes beyond the standard engineering issues covered by most books and delves into structure-function relationships Integration of knowledge across all areas of antibody engineering, development, and marketing Discusses how current and future genetic engineering of cell lines will pave the way for much higher productivity

Handbook of Therapeutic Antibodies Book

Handbook of Therapeutic Antibodies


  • Author : Stefan Dübel
  • Publisher : John Wiley & Sons
  • Release Date : 2014-12-03
  • Genre: Science
  • Pages : 2544
  • ISBN 10 : 9783527329373

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Handbook of Therapeutic Antibodies Excerpt :

Still the most comprehensive reference source on the development, production and therapeutic application of antibodies, this second edition is thoroughly updated and now has 30% more content. Volume I covers selection and engineering strategies for new antibodies, while the second volume look at novel therapeutic concepts and antibodies in clinical trial phases, as well as their potential. Volume III features detailed and specific information about each antibody currently approved for therapeutic purposes, including the clinical data. Beyond providing current knowledge, the authors discuss emerging technologies, future developments, and intellectual property issues, such that this handbook meets the needs of academic researchers, decision makers in industry and healthcare professionals in the clinic.

Introduction to Antibody Engineering Book

Introduction to Antibody Engineering


  • Author : Florian Rüker
  • Publisher : Springer Nature
  • Release Date : 2021-01-04
  • Genre: Medical
  • Pages : 388
  • ISBN 10 : 9783030546304

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Introduction to Antibody Engineering Excerpt :

This highly readable textbook serves as a concise and engaging primer to the emerging field of antibody engineering and its various applications. It introduces readers to the basic science and molecular structure of antibodies, and explores how to characterize and engineer them. Readers will find an overview of the latest methods in antibody identification, improvement and biochemical engineering. Furthermore, alternative antibody formats and bispecific antibodies are discussed. The book’s content is based on lectures for the specializations “Protein Engineering” and “Medical Biotechnology” within the Master’s curriculum in “Biotechnology.” The lectures have been held at the University of Natural Resources and Life Sciences, Vienna, in cooperation with the Medical University of Vienna, since 2012 and are continuously adapted to reflect the latest developments in the field. The book addresses Master’s and PhD students in biotechnology, molecular biology and immunology, and all those who are interested in antibody engineering.

Therapeutic Monoclonal Antibodies Book

Therapeutic Monoclonal Antibodies


  • Author : Zhiqiang An
  • Publisher : John Wiley & Sons
  • Release Date : 2011-09-20
  • Genre: Science
  • Pages : 896
  • ISBN 10 : 9781118210260

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Therapeutic Monoclonal Antibodies Excerpt :

70-chapter authoritative reference that covers therapeutic monoclonal antibody discovery, development, and clinical applications while incorporating principles, experimental data, and methodologies. First book to address the discovery and development of antibody therapeutics in their entirety. Most chapters contain experimental data to illustrate the principles described in them. Authors provide detailed methodologies that readers can take away with them and use in their own laboratories.

Antibody Engineering Book

Antibody Engineering


  • Author : Damien Nevoltris
  • Publisher : Humana
  • Release Date : 2019-12-10
  • Genre: Medical
  • Pages : 543
  • ISBN 10 : 1493993534

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Antibody Engineering Excerpt :

This detailed new edition provides complete and easy access to a variety of antibody engineering techniques. The volume explores topics such as the generation of native, synthetic, or immune antibody libraries, the selection of lead candidates via the different powerful and innovative display technologies, Fc engineering, as well as their production, characterization, and optimization of antibodies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Antibody Engineering: Methods and Protocols, Third Edition presents the reader with an extensive toolbox to create the powerful molecules of tomorrow.

Antibody Engineering Book

Antibody Engineering


  • Author : Thomas Böldicke
  • Publisher : BoD – Books on Demand
  • Release Date : 2018-02-21
  • Genre: Medical
  • Pages : 312
  • ISBN 10 : 9789535138259

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Antibody Engineering Excerpt :

Antibody Engineering comprises in vitro selection and modification of human antibodies including humanization of mouse antibodies for therapy, diagnosis, and research. This book comprises an overview about the generation of antibody diversity and essential techniques in antibody engineering: construction of immune, naive and synthetic libraries, all available in vitro display methods, humanization by chain shuffling, affinity maturation techniques, de novo synthesis of antibody genes, colony assays for library screening, construction of scFvs from hybridomas, and purification of monoclonal antibodies by exclusion chromatography. In addition, other topics that are discussed in this book are application and mechanism of single domain antibodies, structural diversity of antibodies, immune-mediated skin reactions induced by TNF-alpha recombinant antibodies, and bioinformatic approaches to select pathogen-derived peptide sequences for antibody targets.

Antibody Engineering Book

Antibody Engineering


  • Author : J. Donald Capra
  • Publisher : Karger Publishers
  • Release Date : 1997
  • Genre: Science
  • Pages : 212
  • ISBN 10 : 3805563566

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Antibody Engineering Excerpt :

The last decade has witnessed remarkable developments in antibody research and its therapeutic applications. With the methods of molecular biology it is now possible to manipulate the specificities and activities of antibody molecules to generate an almost limitless array of structures for both basic investigations and the clinical setting. The contributions to this volume cover all three domains of the antibody: the variable regions, the relatively neglected but crucial hinge, and the constant region. These studies provide critical structural and functional information about antibodies, while also pointing the way to the construction of molecules with enhanced or even novel properties. Bringing together major experts on antibody engineering, this book is highly recommended to faculty, postdoctoral fellows and graduate students in molecular biology, microbiology, immunology, cancer research and genetics.

Therapeutic Antibodies Book

Therapeutic Antibodies


  • Author : Yuti Chernajovsky
  • Publisher : Springer Science & Business Media
  • Release Date : 2007-12-11
  • Genre: Medical
  • Pages : 380
  • ISBN 10 : 9783540732594

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Therapeutic Antibodies Excerpt :

This essential work, edited by two researchers at London’s famous Queen Mary’s medical school targets one of the most important areas in medical development today. These days, antibody therapeutics are the treatment of choice for several autoimmune and oncological conditions. They are, indeed, becoming the molecules of choice for further combination therapies and cell engineering. In this timely work, a slew of expert in the field of drug development summarize all the current developments and clinical successes.

Antibody Engineering Book
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Antibody Engineering


  • Author : Benny K. C. Lo
  • Publisher : Springer Science & Business Media
  • Release Date : 2004
  • Genre: Medical
  • Pages : 561
  • ISBN 10 : 9781592596669

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Antibody Engineering Excerpt :

A core collection of diverse cutting-edge techniques for the generation, expression, optimization, and characterization of recombinant antibodies. Readily reproducible protocols for lead generation range from the cloning of human immunoglobulin genes to the selection and generation of human recombinant antibodies by humanization approaches, molecular display technologies and transgenic animals. Procedures are also described on restructuring antibody leads into monovalent, multivalent, and bispecific binding fragments for a wide variety of in vivo applications. State-of-the-art technologies are described for the characterization of antigen-binding affinity and specificity with novel applications in radioimmunotargeting, cancer immunotherapy, drug abuse, and proteomics. Use cutting-edge techniques for generating and optimizing recombinant antibodies Generate antibodies by humanization, molecular display technologies and transgenic mice Perform epitope mapping with mass spectrometry, bioinformatics, and array technologies Learn about novel applications in cancer, drug addiction, and proteomics Express recombinant antibodies in bacterial, yeast, insect, mammalian and plant systems.

Monoclonal Antibody Production Book

Monoclonal Antibody Production


  • Author : National Research Council
  • Publisher : National Academies Press
  • Release Date : 1999-06-06
  • Genre: Medical
  • Pages : 74
  • ISBN 10 : 9780309064477

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Monoclonal Antibody Production Excerpt :

The American Anti-Vivisection Society (AAVS) petitioned the National Institutes of Health (NIH) on April 23, 1997, to prohibit the use of animals in the production of mAb. On September 18, 1997, NIH declined to prohibit the use of mice in mAb production, stating that "the ascites method of mAb production is scientifically appropriate for some research projects and cannot be replaced." On March 26, 1998, AAVS submitted a second petition, stating that "NIH failed to provide valid scientific reasons for not supporting a proposed ban." The office of the NIH director asked the National Research Council to conduct a study of methods of producing mAb. In response to that request, the Research Council appointed the Committee on Methods of Producing Monoclonal Antibodies, to act on behalf of the Institute for Laboratory Animal Research of the Commission on Life Sciences, to conduct the study. The 11 expert members of the committee had extensive experience in biomedical research, laboratory animal medicine, animal welfare, pain research, and patient advocacy (Appendix B). The committee was asked to determine whether there was a scientific necessity for the mouse ascites method; if so, whether the method caused pain or distress; and, if so, what could be done to minimize the pain or distress. The committee was also asked to comment on available in vitro methods; to suggest what acceptable scientific rationale, if any, there was for using the mouse ascites method; and to identify regulatory requirements for the continued use of the mouse ascites method. The committee held an open data-gathering meeting during which its members summarized data bearing on those questions. A 1-day workshop (Appendix A) was attended by 34 participants, 14 of whom made formal presentations. A second meeting was held to finalize the report. The present report was written on the basis of information in the literature and information presented at the meeting and the workshop.

Monoclonal Antibodies Book

Monoclonal Antibodies


  • Author : Harleen Kaur
  • Publisher : Elsevier
  • Release Date : 2021-08-03
  • Genre: Medical
  • Pages : 258
  • ISBN 10 : 9780128223192

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Monoclonal Antibodies Excerpt :

Monoclonal antibodies (mAbs) are naturally occurring complex biomolecules. New engineering methods have turned mAbs into a leading therapeutic modality for addressing immunotherapeutic challenges and led to the rise of mAbs as the dominant class of protein therapeutics. mAbs have already demonstrated a great potential in developing safe and reliable treatments for complex diseases and creating more affordable healthcare alternatives. Developing mAbs into well-characterized antibody therapeutics that meet regulatory expectations, however, is extremely challenging. Obstacles to overcome include the determination and development of physiochemical characteristics such as aggregation, fragmentation, charge variants, identity, carbohydrate structure, and higher-order structure (HOS). This book dives deep into mAbs structure and the array of physiochemical testing and characterization methods that need to be developed and validated to establish a mAb as a therapeutic molecule. The main focus of this book is on physiochemical aspects, including the importance of establishing quality attributes such as glycosylation, primary sequence, purity, and HOS and elucidating the structure of new antibody formats by mass spectrometry. Each of the aforementioned quality attributes has been discussed in detail; this will help scientists in researching and developing biopharmaceuticals and biosimilars to find practical solutions to physicochemical testing and characterization. Describes the spectrum of analytical tests and characterization methods necessary for developing and releasing mAb batches Details antibody heterogeneity in terms of size, charge, and carbohydrate content Gives special focus to the structural analysis of mAbs, including mass spectrometry analysis Presents the basic structure of mAbs with clarity and rigor Addresses regulatory guidelines - including ICH Q6B - in relation to quality attributes Lays out characterization and development case studies including biosimila

Protein Therapeutics  2 Volume Set Book

Protein Therapeutics 2 Volume Set


  • Author : Tristan Vaughan
  • Publisher : John Wiley & Sons
  • Release Date : 2017-12-04
  • Genre: Medical
  • Pages : 768
  • ISBN 10 : 9783527340866

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Protein Therapeutics 2 Volume Set Excerpt :

In this practice-oriented handbook, professionals from some of the largest biopharmaceutical companies and top academic researchers address the key concepts and challenges in the development of protein pharmaceuticals for medicinal chemists and other professionals working in drug development. Following an introduction tracing the rapid development of the protein therapeutics market over the last decade, all currently used therapeutic protein scaffolds are surveyed, from human and non-human antibodies to antibody mimetics, bispecific antibodies and antibody-drug conjugates. This ready reference then goes on to review other key aspects such as pharmacokinetics, safety and immunogenicity, manufacture, formulation and delivery. The handbook then takes a look at current key clinical applications for protein therapeutics, from respiratory and inflammation to oncology and immune-oncology, infectious diseases and rescue therapy. Finally, several exciting prospects for the future of protein therapeutics are highlighted and discussed.

Antibody Drug Discovery Book

Antibody Drug Discovery


  • Author : Clive R. Wood
  • Publisher : World Scientific
  • Release Date : 2012
  • Genre: Medical
  • Pages : 473
  • ISBN 10 : 9781848166288

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Antibody Drug Discovery Excerpt :

Antibody-based therapeutics are a central driver of the success of biopharmaceuticals. The discovery technology of this field is isolated to a limited number of centers of excellence in industry and academia. The objective of this volume is to provide a series of guides to those evaluating and preparing to enter particular areas within the field. Each chapter is written with a historical perspective that sets into context the significance of the key developments, and with the provision of “points to consider” for the reader as a value-added feature of the volume. All contributors are experts in their fields and have played pivotal roles in the creation of the technology.

Biosimilars of Monoclonal Antibodies Book

Biosimilars of Monoclonal Antibodies


  • Author : Cheng Liu
  • Publisher : John Wiley & Sons
  • Release Date : 2016-12-12
  • Genre: Medical
  • Pages : 704
  • ISBN 10 : 9781118940631

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Biosimilars of Monoclonal Antibodies Excerpt :

Addressing a significant need by describing the science and process involved to develop biosimilars of monoclonal antibody (mAb) drugs, this book covers all aspects of biosimilar development: preclinical, clinical, regulatory, manufacturing. • Guides readers through the complex landscape involved with developing biosimilar versions of monoclonal antibody (mAb) drugs • Features flow charts, tables, and figures that clearly illustrate processes and makes the book comprehensible and accessible • Includes a review of FDA-approved mAb drugs as a quick reference to facts and useful information • Examines new technologies and strategies for improving biosimilar mAbs

Protein Engineering Book

Protein Engineering


  • Author : Huimin Zhao
  • Publisher : John Wiley & Sons
  • Release Date : 2021-08-23
  • Genre: Science
  • Pages : 432
  • ISBN 10 : 9783527344703

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Protein Engineering Excerpt :

A one-stop reference that reviews protein design strategies to applications in industrial and medical biotechnology Protein Engineering: Tools and Applications is a comprehensive resource that offers a systematic and comprehensive review of the most recent advances in the field, and contains detailed information on the methodologies and strategies behind these approaches. The authors—noted experts on the topic—explore the distinctive advantages and disadvantages of the presented methodologies and strategies in a targeted and focused manner that allows for the adaptation and implementation of the strategies for new applications. The book contains information on the directed evolution, rational design, and semi-rational design of proteins and offers a review of the most recent applications in industrial and medical biotechnology. This important book: Covers technologies and methodologies used in protein engineering Includes the strategies behind the approaches, designed to help with the adaptation and implementation of these strategies for new applications Offers a comprehensive and thorough treatment of protein engineering from primary strategies to applications in industrial and medical biotechnology Presents cutting edge advances in the continuously evolving field of protein engineering Written for students and professionals of bioengineering, biotechnology, biochemistry, Protein Engineering: Tools and Applications offers an essential resource to the design strategies in protein engineering and reviews recent applications.